A novel antioxidant gene from Mycobacterium tuberculosis
Among the major antimicrobial products of macrophages are reactive intermediates of the oxidation of nitrogen (RNI) and the reduction of oxygen (ROI). Selection of recombinants in acidified nitrite led to the cloning of a novel gene, noxR1, from a pathogenic clinical isolate of Mycobacterium tubercu...
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| Veröffentlicht in: | The Journal of experimental medicine 1997-12, Vol.186 (11), p.1885-1896 |
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| container_end_page | 1896 |
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| container_issue | 11 |
| container_start_page | 1885 |
| container_title | The Journal of experimental medicine |
| container_volume | 186 |
| creator | Ehrt, S Shiloh, M U Ruan, J Choi, M Gunzburg, S Nathan, C Xie, Q Riley, L W |
| description | Among the major antimicrobial products of macrophages are reactive intermediates of the oxidation of nitrogen (RNI) and the reduction of oxygen (ROI). Selection of recombinants in acidified nitrite led to the cloning of a novel gene, noxR1, from a pathogenic clinical isolate of Mycobacterium tuberculosis. Expression of noxR1 conferred upon Escherichia coli and Mycobacterium smegmatis enhanced ability to resist RNI and ROI, whether the bacteria were exposed to exogenous compounds in medium or to endogenous products in macrophages. These studies provide the first identification of an RNI resistance mechanism in mycobacteria, point to a new mechanism for resistance to ROI, and raise the possibility that inhibition of the noxR1 pathway might enhance the ability of macrophages to control tuberculosis. |
| doi_str_mv | 10.1084/jem.186.11.1885 |
| format | Article |
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Selection of recombinants in acidified nitrite led to the cloning of a novel gene, noxR1, from a pathogenic clinical isolate of Mycobacterium tuberculosis. Expression of noxR1 conferred upon Escherichia coli and Mycobacterium smegmatis enhanced ability to resist RNI and ROI, whether the bacteria were exposed to exogenous compounds in medium or to endogenous products in macrophages. 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| identifier | ISSN: 0022-1007 |
| ispartof | The Journal of experimental medicine, 1997-12, Vol.186 (11), p.1885-1896 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Animals Antioxidants Bacterial Proteins - genetics Bacterial Proteins - physiology Base Sequence Cloning, Molecular DNA, Bacterial - genetics Escherichia coli - genetics Genes, Bacterial Hydrogen Peroxide - metabolism Macrophages - metabolism Macrophages - microbiology Mice Mice, Inbred C57BL Molecular Sequence Data Mycobacterium - genetics Mycobacterium tuberculosis - genetics Nitrites - metabolism Oxidation-Reduction Reactive Oxygen Species - metabolism Recombinant Fusion Proteins - metabolism Transfection Tuberculosis - immunology |
| title | A novel antioxidant gene from Mycobacterium tuberculosis |
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