A REAL-TIME PCR-BASED ASSAY FOR DETECTION OF WUCHERERIA BANCROFTI DNA IN BLOOD AND MOSQUITOES

We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting Wuchereria bancrofti DNA in human blood and in mosquitoes. An assay based on detection of the W. bancrofti "LDR" repeat DNA sequence was more sensitive than an assay for Wolbachia 16S rDNA. The LDR-ba...

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Veröffentlicht in:	The American journal of tropical medicine and hygiene 2006-05, Vol.74 (5), p.826-832
Hauptverfasser:	RAO, RAMAKRISHNA U, ATKINSON, LAURA J, RAMZY, REDA M. R, HELMY, HANAN, FARID, HODA A, BOCKARIE, MOSES. J, SUSAPU, MELINDA, LANEY, SANDRA J, WILLIAMS, STEVEN A, WEIL, GARY J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anopheles - parasitology Biological and medical sciences Culex - parasitology Culicidae Diseases caused by nematodes DNA Primers DNA, Bacterial - analysis DNA, Protozoan - analysis Egypt Filariases Filariasis - blood Filariasis - diagnosis Filariasis - transmission Helminthic diseases Humans Infectious diseases Insect Vectors - parasitology Lymphatic filariases Medical sciences Papua New Guinea Parasitic diseases Polymerase Chain Reaction Predictive Value of Tests Reagent Kits, Diagnostic RNA, Ribosomal, 16S - analysis Sensitivity and Specificity Wolbachia Wolbachia - genetics Wolbachia - isolation & purification Wuchereria bancrofti Wuchereria bancrofti - genetics Wuchereria bancrofti - isolation & purification
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