Putative DNA Quadruplex Formation within the Human c-kit Oncogene
The DNA sequence, d(AGGGAGGGCGCTGGGAGGAGGG), occurs within the promoter region of the c-kit oncogene. We show here, using a combination of NMR, circular dichroism, and melting temperature measurements, that this sequence forms a four-stranded quadruplex structure under physiological conditions. Vari...
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| Veröffentlicht in: | Journal of the American Chemical Society 2005-08, Vol.127 (30), p.10584-10589 |
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| container_end_page | 10589 |
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| container_issue | 30 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 127 |
| creator | Rankin, Sarah Reszka, Anthony P Huppert, Julian Zloh, Mire Parkinson, Gary N Todd, Alan K Ladame, Sylvain Balasubramanian, Shankar Neidle, Stephen |
| description | The DNA sequence, d(AGGGAGGGCGCTGGGAGGAGGG), occurs within the promoter region of the c-kit oncogene. We show here, using a combination of NMR, circular dichroism, and melting temperature measurements, that this sequence forms a four-stranded quadruplex structure under physiological conditions. Variations in the sequences that intervene between the guanine tracts have been examined, and surprisingly, none of these modified sequences forms a quadruplex arrangement under these conditions. This suggests that the occurrence of quadruplex-forming sequences within the human and other genomes is less than was hitherto expected. The c-kit quadruplex may be a new target for therapeutic intervention in cancers where there is elevated expression of the c-kit gene. |
| doi_str_mv | 10.1021/ja050823u |
| format | Article |
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We show here, using a combination of NMR, circular dichroism, and melting temperature measurements, that this sequence forms a four-stranded quadruplex structure under physiological conditions. Variations in the sequences that intervene between the guanine tracts have been examined, and surprisingly, none of these modified sequences forms a quadruplex arrangement under these conditions. This suggests that the occurrence of quadruplex-forming sequences within the human and other genomes is less than was hitherto expected. The c-kit quadruplex may be a new target for therapeutic intervention in cancers where there is elevated expression of the c-kit gene.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja050823u</identifier><identifier>PMID: 16045346</identifier><identifier>CODEN: JACSAT</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Base Sequence ; Biological and medical sciences ; Circular Dichroism ; DNA - chemistry ; DNA - genetics ; DNA - metabolism ; Fundamental and applied biological sciences. Psychology ; G-Quadruplexes ; Humans ; Molecular biophysics ; Nuclear Magnetic Resonance, Biomolecular ; Oncogenes - genetics ; Promoter Regions, Genetic ; Proto-Oncogene Proteins c-kit - genetics ; Structure in molecular biology ; Supramolecular structure</subject><ispartof>Journal of the American Chemical Society, 2005-08, Vol.127 (30), p.10584-10589</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2005 INIST-CNRS</rights><rights>2005 American Chemical Society 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a502t-684d0675017de709bd880af1e6f123a8f0eb4eaa334ce30ccdaa82322ef4efc23</citedby><cites>FETCH-LOGICAL-a502t-684d0675017de709bd880af1e6f123a8f0eb4eaa334ce30ccdaa82322ef4efc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja050823u$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja050823u$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,315,781,785,886,2766,27078,27926,27927,56740,56790</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17021319$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16045346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rankin, Sarah</creatorcontrib><creatorcontrib>Reszka, Anthony P</creatorcontrib><creatorcontrib>Huppert, Julian</creatorcontrib><creatorcontrib>Zloh, Mire</creatorcontrib><creatorcontrib>Parkinson, Gary N</creatorcontrib><creatorcontrib>Todd, Alan K</creatorcontrib><creatorcontrib>Ladame, Sylvain</creatorcontrib><creatorcontrib>Balasubramanian, Shankar</creatorcontrib><creatorcontrib>Neidle, Stephen</creatorcontrib><title>Putative DNA Quadruplex Formation within the Human c-kit Oncogene</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>The DNA sequence, d(AGGGAGGGCGCTGGGAGGAGGG), occurs within the promoter region of the c-kit oncogene. We show here, using a combination of NMR, circular dichroism, and melting temperature measurements, that this sequence forms a four-stranded quadruplex structure under physiological conditions. Variations in the sequences that intervene between the guanine tracts have been examined, and surprisingly, none of these modified sequences forms a quadruplex arrangement under these conditions. This suggests that the occurrence of quadruplex-forming sequences within the human and other genomes is less than was hitherto expected. The c-kit quadruplex may be a new target for therapeutic intervention in cancers where there is elevated expression of the c-kit gene.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Circular Dichroism</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G-Quadruplexes</subject><subject>Humans</subject><subject>Molecular biophysics</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Oncogenes - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Proto-Oncogene Proteins c-kit - genetics</subject><subject>Structure in molecular biology</subject><subject>Supramolecular structure</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1v0zAYhS3ExMrggj-AcgMSF9n8EdvJDVK1sQ2p2goMcWm9dd6s7hKns5Mx_v2MWrUgIXFlWefRo2MfQt4weswoZycroJKWXIzPyIRJTnPJuHpOJpRSnutSiUPyMsZVuha8ZC_IIVO0kKJQEzKdjwMM7gGzs6tp9mWEOozrFh-z8z50Keh99tMNS-ezYYnZ5diBz2x-54bs2tv-Fj2-IgcNtBFfb88j8v38083pZT67vvh8Op3lICkfclUWNVVaUqZr1LRa1GVJoWGoGsYFlA3FRYEAQhQWBbW2BkhP4hybAhvLxRH5uPGux0WHtUU_BGjNOrgOwi_TgzN_J94tzW3_YDirZFmpJHi_FYT-fsQ4mM5Fi20LHvsxGlWmP6w4-y_ItKxoIXQCP2xAG_oYAza7Noya38uY3TKJfftn_T25nSIB77YARAttE8BbF_ecTj7BqsTlG87FAR93OYQ7o7TQ0tzMv5mKFXM5-_rDnO29YKNZ9WPwaaV_FHwCHkOxOw</recordid><startdate>20050803</startdate><enddate>20050803</enddate><creator>Rankin, Sarah</creator><creator>Reszka, Anthony P</creator><creator>Huppert, Julian</creator><creator>Zloh, Mire</creator><creator>Parkinson, Gary N</creator><creator>Todd, Alan K</creator><creator>Ladame, Sylvain</creator><creator>Balasubramanian, Shankar</creator><creator>Neidle, Stephen</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050803</creationdate><title>Putative DNA Quadruplex Formation within the Human c-kit Oncogene</title><author>Rankin, Sarah ; Reszka, Anthony P ; Huppert, Julian ; Zloh, Mire ; Parkinson, Gary N ; Todd, Alan K ; Ladame, Sylvain ; Balasubramanian, Shankar ; Neidle, Stephen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a502t-684d0675017de709bd880af1e6f123a8f0eb4eaa334ce30ccdaa82322ef4efc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Circular Dichroism</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G-Quadruplexes</topic><topic>Humans</topic><topic>Molecular biophysics</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>Oncogenes - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Proto-Oncogene Proteins c-kit - genetics</topic><topic>Structure in molecular biology</topic><topic>Supramolecular structure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rankin, Sarah</creatorcontrib><creatorcontrib>Reszka, Anthony P</creatorcontrib><creatorcontrib>Huppert, Julian</creatorcontrib><creatorcontrib>Zloh, Mire</creatorcontrib><creatorcontrib>Parkinson, Gary N</creatorcontrib><creatorcontrib>Todd, Alan K</creatorcontrib><creatorcontrib>Ladame, Sylvain</creatorcontrib><creatorcontrib>Balasubramanian, Shankar</creatorcontrib><creatorcontrib>Neidle, Stephen</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rankin, Sarah</au><au>Reszka, Anthony P</au><au>Huppert, Julian</au><au>Zloh, Mire</au><au>Parkinson, Gary N</au><au>Todd, Alan K</au><au>Ladame, Sylvain</au><au>Balasubramanian, Shankar</au><au>Neidle, Stephen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Putative DNA Quadruplex Formation within the Human c-kit Oncogene</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2005-08-03</date><risdate>2005</risdate><volume>127</volume><issue>30</issue><spage>10584</spage><epage>10589</epage><pages>10584-10589</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><coden>JACSAT</coden><abstract>The DNA sequence, d(AGGGAGGGCGCTGGGAGGAGGG), occurs within the promoter region of the c-kit oncogene. We show here, using a combination of NMR, circular dichroism, and melting temperature measurements, that this sequence forms a four-stranded quadruplex structure under physiological conditions. Variations in the sequences that intervene between the guanine tracts have been examined, and surprisingly, none of these modified sequences forms a quadruplex arrangement under these conditions. This suggests that the occurrence of quadruplex-forming sequences within the human and other genomes is less than was hitherto expected. The c-kit quadruplex may be a new target for therapeutic intervention in cancers where there is elevated expression of the c-kit gene.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16045346</pmid><doi>10.1021/ja050823u</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Base Sequence Biological and medical sciences Circular Dichroism DNA - chemistry DNA - genetics DNA - metabolism Fundamental and applied biological sciences. Psychology G-Quadruplexes Humans Molecular biophysics Nuclear Magnetic Resonance, Biomolecular Oncogenes - genetics Promoter Regions, Genetic Proto-Oncogene Proteins c-kit - genetics Structure in molecular biology Supramolecular structure |
| title | Putative DNA Quadruplex Formation within the Human c-kit Oncogene |
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