Human autoantibody to defensin: disease association with hyperreactive onchocerciasis (sowda)
Chronic hyperreactive onchodermatitis (sowda) is a severe form of onchocerciasis observed in a subset of individuals infected with the filarial nematode Onchocerca volvulus. SDS-PAGE and immunoblot analyses of O. volvulus adult worm extracts were used to characterize the antigens of the marked antib...
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| Veröffentlicht in: | The Journal of experimental medicine 1995-07, Vol.182 (1), p.41-47 |
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| description | Chronic hyperreactive onchodermatitis (sowda) is a severe form of onchocerciasis observed in a subset of individuals infected with the filarial nematode Onchocerca volvulus. SDS-PAGE and immunoblot analyses of O. volvulus adult worm extracts were used to characterize the antigens of the marked antibody response of sowda patients. One 2.5-kD antigen was recognized by sera from all 35(100%) sowda patients that were studied. In comparison, only 7 of 44 (16%) patients with generalized onchocerciasis and 11 of 21 (52%) of exposed individuals with no microfilariae in skin snips and no signs of disease showed reactivity to this antigen. Microfilaricidal treatment of sowda patients with improvement of the clinical status was associated with a decrease or disappearance of antibodies to the 2.5-kD antigen. Amino acid sequencing of the antigen indicated identity to human defensins 1-3 of neutrophils. Defensin was demonstrated by immunohistochemical staining in onchocercal nodules on the surface of adult filariae and in the surrounding tissue. A similar staining pattern was observed for other proteins present in neutrophils such as myeloperoxidase, elastase, and the L-1 protein complex (MRP 8/MRP 14), indicating that neutrophils, macrophages, and their proteins predominate in the environment adjacent to the worms. These results demonstrate an association between the presence of autoantibodies to defensins and an infectious disease of known etiology. The association with a particular form of onchocerciasis, sowda, suggests a link between formation of autoantibodies to defensin and enhanced immune reactivity towards the parasite. |
| doi_str_mv | 10.1084/jem.182.1.41 |
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SDS-PAGE and immunoblot analyses of O. volvulus adult worm extracts were used to characterize the antigens of the marked antibody response of sowda patients. One 2.5-kD antigen was recognized by sera from all 35(100%) sowda patients that were studied. In comparison, only 7 of 44 (16%) patients with generalized onchocerciasis and 11 of 21 (52%) of exposed individuals with no microfilariae in skin snips and no signs of disease showed reactivity to this antigen. Microfilaricidal treatment of sowda patients with improvement of the clinical status was associated with a decrease or disappearance of antibodies to the 2.5-kD antigen. Amino acid sequencing of the antigen indicated identity to human defensins 1-3 of neutrophils. Defensin was demonstrated by immunohistochemical staining in onchocercal nodules on the surface of adult filariae and in the surrounding tissue. A similar staining pattern was observed for other proteins present in neutrophils such as myeloperoxidase, elastase, and the L-1 protein complex (MRP 8/MRP 14), indicating that neutrophils, macrophages, and their proteins predominate in the environment adjacent to the worms. These results demonstrate an association between the presence of autoantibodies to defensins and an infectious disease of known etiology. The association with a particular form of onchocerciasis, sowda, suggests a link between formation of autoantibodies to defensin and enhanced immune reactivity towards the parasite.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.182.1.41</identifier><identifier>PMID: 7790822</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Adolescent ; Adult ; Aged ; Amino Acid Sequence ; Animals ; Antibodies, Helminth - analysis ; Antigens, Helminth - chemistry ; Antigens, Helminth - immunology ; Autoantibodies - immunology ; Autoantigens - chemistry ; Autoantigens - immunology ; Autoimmune Diseases - etiology ; Autoimmune Diseases - immunology ; Autoimmune Diseases - parasitology ; Blood Proteins - chemistry ; Blood Proteins - immunology ; Child ; Child, Preschool ; Chronic Disease ; Cross Reactions ; Defensins ; Dermatitis - etiology ; Dermatitis - immunology ; Dermatitis - parasitology ; Female ; Humans ; Male ; Microfilariae - isolation & purification ; Middle Aged ; Molecular Mimicry ; Molecular Sequence Data ; Neutrophils - chemistry ; Neutrophils - immunology ; Onchocerca volvulus ; Onchocerca volvulus - immunology ; Onchocerca volvulus - isolation & purification ; Onchocerciasis - complications ; Onchocerciasis - immunology ; Sequence Homology, Amino Acid ; Skin - parasitology</subject><ispartof>The Journal of experimental medicine, 1995-07, Vol.182 (1), p.41-47</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-f7195c39f67b1d878f2aea513bba8dcd6d9146339572a30d8f70712faa16fab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7790822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gallin, M Y</creatorcontrib><creatorcontrib>Jacobi, A B</creatorcontrib><creatorcontrib>Büttner, D W</creatorcontrib><creatorcontrib>Schönberger, O</creatorcontrib><creatorcontrib>Marti, T</creatorcontrib><creatorcontrib>Erttmann, K D</creatorcontrib><title>Human autoantibody to defensin: disease association with hyperreactive onchocerciasis (sowda)</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>Chronic hyperreactive onchodermatitis (sowda) is a severe form of onchocerciasis observed in a subset of individuals infected with the filarial nematode Onchocerca volvulus. SDS-PAGE and immunoblot analyses of O. volvulus adult worm extracts were used to characterize the antigens of the marked antibody response of sowda patients. One 2.5-kD antigen was recognized by sera from all 35(100%) sowda patients that were studied. In comparison, only 7 of 44 (16%) patients with generalized onchocerciasis and 11 of 21 (52%) of exposed individuals with no microfilariae in skin snips and no signs of disease showed reactivity to this antigen. Microfilaricidal treatment of sowda patients with improvement of the clinical status was associated with a decrease or disappearance of antibodies to the 2.5-kD antigen. Amino acid sequencing of the antigen indicated identity to human defensins 1-3 of neutrophils. Defensin was demonstrated by immunohistochemical staining in onchocercal nodules on the surface of adult filariae and in the surrounding tissue. A similar staining pattern was observed for other proteins present in neutrophils such as myeloperoxidase, elastase, and the L-1 protein complex (MRP 8/MRP 14), indicating that neutrophils, macrophages, and their proteins predominate in the environment adjacent to the worms. These results demonstrate an association between the presence of autoantibodies to defensins and an infectious disease of known etiology. The association with a particular form of onchocerciasis, sowda, suggests a link between formation of autoantibodies to defensin and enhanced immune reactivity towards the parasite.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Helminth - analysis</subject><subject>Antigens, Helminth - chemistry</subject><subject>Antigens, Helminth - immunology</subject><subject>Autoantibodies - immunology</subject><subject>Autoantigens - chemistry</subject><subject>Autoantigens - immunology</subject><subject>Autoimmune Diseases - etiology</subject><subject>Autoimmune Diseases - immunology</subject><subject>Autoimmune Diseases - parasitology</subject><subject>Blood Proteins - chemistry</subject><subject>Blood Proteins - immunology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Chronic Disease</subject><subject>Cross Reactions</subject><subject>Defensins</subject><subject>Dermatitis - etiology</subject><subject>Dermatitis - immunology</subject><subject>Dermatitis - parasitology</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Microfilariae - isolation & purification</subject><subject>Middle Aged</subject><subject>Molecular Mimicry</subject><subject>Molecular Sequence Data</subject><subject>Neutrophils - chemistry</subject><subject>Neutrophils - immunology</subject><subject>Onchocerca volvulus</subject><subject>Onchocerca volvulus - immunology</subject><subject>Onchocerca volvulus - isolation & purification</subject><subject>Onchocerciasis - complications</subject><subject>Onchocerciasis - immunology</subject><subject>Sequence Homology, Amino Acid</subject><subject>Skin - parasitology</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEUgIModa3evAo5iYKz5uXHJONBkNJaoeClVwlv8sNN2ZmsSaZl_3u3dil68vQO7-PjPT5CXgNbAzPy402Y1mD4GtYSnpAVKMm6QQnzlKwY47wDxvRz8qLWG8ZAStWfkBOtB2Y4X5Efl8uEM8WlZZxbGrPf05apDzHMNc2fqE81YA0Ua80uYUt5pnepbehmvwulBHQt3QaaZ7fJLpQDUlOl72q-8_j-JXkWcVvDq-M8JdcX59dnl93V96_fzr5cdU4Ca13UMCgnhtjrEbzRJnIMqECMIxrvfO8HkL0Qg9IcBfMmaqaBR0ToI47ilHx-0O6WcQrehbkV3NpdSROWvc2Y7L-bOW3sz3xrOQycGTgI3h4FJf9aQm12StWF7RbnkJdqtRYKlFL_BaE3Qsk_xg8PoCu51hLi4zXA7H02e8hmD9ksWHmPv_n7g0f42En8BjfPlkc</recordid><startdate>19950701</startdate><enddate>19950701</enddate><creator>Gallin, M Y</creator><creator>Jacobi, A B</creator><creator>Büttner, D W</creator><creator>Schönberger, O</creator><creator>Marti, T</creator><creator>Erttmann, K D</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950701</creationdate><title>Human autoantibody to defensin: disease association with hyperreactive onchocerciasis (sowda)</title><author>Gallin, M Y ; Jacobi, A B ; Büttner, D W ; Schönberger, O ; Marti, T ; Erttmann, K D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-f7195c39f67b1d878f2aea513bba8dcd6d9146339572a30d8f70712faa16fab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Helminth - analysis</topic><topic>Antigens, Helminth - chemistry</topic><topic>Antigens, Helminth - immunology</topic><topic>Autoantibodies - immunology</topic><topic>Autoantigens - chemistry</topic><topic>Autoantigens - immunology</topic><topic>Autoimmune Diseases - etiology</topic><topic>Autoimmune Diseases - immunology</topic><topic>Autoimmune Diseases - parasitology</topic><topic>Blood Proteins - chemistry</topic><topic>Blood Proteins - immunology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Chronic Disease</topic><topic>Cross Reactions</topic><topic>Defensins</topic><topic>Dermatitis - etiology</topic><topic>Dermatitis - immunology</topic><topic>Dermatitis - parasitology</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Microfilariae - isolation & purification</topic><topic>Middle Aged</topic><topic>Molecular Mimicry</topic><topic>Molecular Sequence Data</topic><topic>Neutrophils - chemistry</topic><topic>Neutrophils - immunology</topic><topic>Onchocerca volvulus</topic><topic>Onchocerca volvulus - immunology</topic><topic>Onchocerca volvulus - isolation & purification</topic><topic>Onchocerciasis - complications</topic><topic>Onchocerciasis - immunology</topic><topic>Sequence Homology, Amino Acid</topic><topic>Skin - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallin, M Y</creatorcontrib><creatorcontrib>Jacobi, A B</creatorcontrib><creatorcontrib>Büttner, D W</creatorcontrib><creatorcontrib>Schönberger, O</creatorcontrib><creatorcontrib>Marti, T</creatorcontrib><creatorcontrib>Erttmann, K D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallin, M Y</au><au>Jacobi, A B</au><au>Büttner, D W</au><au>Schönberger, O</au><au>Marti, T</au><au>Erttmann, K D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human autoantibody to defensin: disease association with hyperreactive onchocerciasis (sowda)</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>1995-07-01</date><risdate>1995</risdate><volume>182</volume><issue>1</issue><spage>41</spage><epage>47</epage><pages>41-47</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>Chronic hyperreactive onchodermatitis (sowda) is a severe form of onchocerciasis observed in a subset of individuals infected with the filarial nematode Onchocerca volvulus. SDS-PAGE and immunoblot analyses of O. volvulus adult worm extracts were used to characterize the antigens of the marked antibody response of sowda patients. One 2.5-kD antigen was recognized by sera from all 35(100%) sowda patients that were studied. In comparison, only 7 of 44 (16%) patients with generalized onchocerciasis and 11 of 21 (52%) of exposed individuals with no microfilariae in skin snips and no signs of disease showed reactivity to this antigen. Microfilaricidal treatment of sowda patients with improvement of the clinical status was associated with a decrease or disappearance of antibodies to the 2.5-kD antigen. Amino acid sequencing of the antigen indicated identity to human defensins 1-3 of neutrophils. Defensin was demonstrated by immunohistochemical staining in onchocercal nodules on the surface of adult filariae and in the surrounding tissue. A similar staining pattern was observed for other proteins present in neutrophils such as myeloperoxidase, elastase, and the L-1 protein complex (MRP 8/MRP 14), indicating that neutrophils, macrophages, and their proteins predominate in the environment adjacent to the worms. These results demonstrate an association between the presence of autoantibodies to defensins and an infectious disease of known etiology. The association with a particular form of onchocerciasis, sowda, suggests a link between formation of autoantibodies to defensin and enhanced immune reactivity towards the parasite.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>7790822</pmid><doi>10.1084/jem.182.1.41</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of experimental medicine, 1995-07, Vol.182 (1), p.41-47 |
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| subjects | Adolescent Adult Aged Amino Acid Sequence Animals Antibodies, Helminth - analysis Antigens, Helminth - chemistry Antigens, Helminth - immunology Autoantibodies - immunology Autoantigens - chemistry Autoantigens - immunology Autoimmune Diseases - etiology Autoimmune Diseases - immunology Autoimmune Diseases - parasitology Blood Proteins - chemistry Blood Proteins - immunology Child Child, Preschool Chronic Disease Cross Reactions Defensins Dermatitis - etiology Dermatitis - immunology Dermatitis - parasitology Female Humans Male Microfilariae - isolation & purification Middle Aged Molecular Mimicry Molecular Sequence Data Neutrophils - chemistry Neutrophils - immunology Onchocerca volvulus Onchocerca volvulus - immunology Onchocerca volvulus - isolation & purification Onchocerciasis - complications Onchocerciasis - immunology Sequence Homology, Amino Acid Skin - parasitology |
| title | Human autoantibody to defensin: disease association with hyperreactive onchocerciasis (sowda) |
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