Persistence of human T cell lymphotropic virus type 1 (HTLV-1) sequences in peripheral blood mononuclear cells from patients with mycosis fungoides
Mycosis fungoides (MF) is a rare form of cutaneous T cell lymphoma suspected of having a viral etiology. As in adult T cell leukemia, the virus involved may be human T lymphotropic virus type 1 (HTLV-1). We cultured the peripheral blood mononuclear cells (PBMC) of 29 patients with MF HTLV-1 seronega...
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| Veröffentlicht in: | The Journal of experimental medicine 1994-11, Vol.180 (5), p.1973-1978 |
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| container_end_page | 1978 |
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| container_issue | 5 |
| container_start_page | 1973 |
| container_title | The Journal of experimental medicine |
| container_volume | 180 |
| creator | Manca, N Piacentini, E Gelmi, M Calzavara, P Manganoni, M A Glukhov, A Gargiulo, F De Francesco, M Pirali, F De Panfilis, G Turano, A |
| description | Mycosis fungoides (MF) is a rare form of cutaneous T cell lymphoma suspected of having a viral etiology. As in adult T cell leukemia, the virus involved may be human T lymphotropic virus type 1 (HTLV-1). We cultured the peripheral blood mononuclear cells (PBMC) of 29 patients with MF HTLV-1 seronegative by enzyme-linked immunosorbent assay and Western blot. The presence of reverse transcriptase (RT) and p24 antigen was investigated in the concentrate supernatant of the culture. The DNA of all studied patients was submitted to polymerase chain reaction and Southern blot analysis using primers and probes recognizing the tax region of HTLV-1/2 and the pol region of HTLV-1. 10 of 29 patients were found positive to HTLV-1, whereas they were always negative to RT and p24. The same results were confirmed in double blind after 6 mo. Our findings suggest HTLV-1 may be involved in the etiology of MF, at least in certain cases. |
| doi_str_mv | 10.1084/jem.180.5.1973 |
| format | Article |
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As in adult T cell leukemia, the virus involved may be human T lymphotropic virus type 1 (HTLV-1). We cultured the peripheral blood mononuclear cells (PBMC) of 29 patients with MF HTLV-1 seronegative by enzyme-linked immunosorbent assay and Western blot. The presence of reverse transcriptase (RT) and p24 antigen was investigated in the concentrate supernatant of the culture. The DNA of all studied patients was submitted to polymerase chain reaction and Southern blot analysis using primers and probes recognizing the tax region of HTLV-1/2 and the pol region of HTLV-1. 10 of 29 patients were found positive to HTLV-1, whereas they were always negative to RT and p24. The same results were confirmed in double blind after 6 mo. Our findings suggest HTLV-1 may be involved in the etiology of MF, at least in certain cases.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.180.5.1973</identifier><identifier>PMID: 7964473</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Adult ; Aged ; Aged, 80 and over ; AIDS/HIV ; Base Sequence ; Cells, Cultured ; DNA, Viral - analysis ; Genes, pX ; Herpesvirus 4, Human - genetics ; Herpesvirus 4, Human - isolation & purification ; Human T-lymphotropic virus 1 - genetics ; Human T-lymphotropic virus 1 - isolation & purification ; Humans ; Leukocytes, Mononuclear - virology ; Middle Aged ; Molecular Sequence Data ; Mycosis Fungoides - blood ; Mycosis Fungoides - virology ; Polymerase Chain Reaction ; Skin Neoplasms - blood ; Skin Neoplasms - virology</subject><ispartof>The Journal of experimental medicine, 1994-11, Vol.180 (5), p.1973-1978</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-579d16475b541ef5c0ff43bb98339e89bb0ec85989218407fa55165f2737123f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7964473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manca, N</creatorcontrib><creatorcontrib>Piacentini, E</creatorcontrib><creatorcontrib>Gelmi, M</creatorcontrib><creatorcontrib>Calzavara, P</creatorcontrib><creatorcontrib>Manganoni, M A</creatorcontrib><creatorcontrib>Glukhov, A</creatorcontrib><creatorcontrib>Gargiulo, F</creatorcontrib><creatorcontrib>De Francesco, M</creatorcontrib><creatorcontrib>Pirali, F</creatorcontrib><creatorcontrib>De Panfilis, G</creatorcontrib><creatorcontrib>Turano, A</creatorcontrib><title>Persistence of human T cell lymphotropic virus type 1 (HTLV-1) sequences in peripheral blood mononuclear cells from patients with mycosis fungoides</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>Mycosis fungoides (MF) is a rare form of cutaneous T cell lymphoma suspected of having a viral etiology. As in adult T cell leukemia, the virus involved may be human T lymphotropic virus type 1 (HTLV-1). We cultured the peripheral blood mononuclear cells (PBMC) of 29 patients with MF HTLV-1 seronegative by enzyme-linked immunosorbent assay and Western blot. The presence of reverse transcriptase (RT) and p24 antigen was investigated in the concentrate supernatant of the culture. The DNA of all studied patients was submitted to polymerase chain reaction and Southern blot analysis using primers and probes recognizing the tax region of HTLV-1/2 and the pol region of HTLV-1. 10 of 29 patients were found positive to HTLV-1, whereas they were always negative to RT and p24. The same results were confirmed in double blind after 6 mo. Our findings suggest HTLV-1 may be involved in the etiology of MF, at least in certain cases.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>AIDS/HIV</subject><subject>Base Sequence</subject><subject>Cells, Cultured</subject><subject>DNA, Viral - analysis</subject><subject>Genes, pX</subject><subject>Herpesvirus 4, Human - genetics</subject><subject>Herpesvirus 4, Human - isolation & purification</subject><subject>Human T-lymphotropic virus 1 - genetics</subject><subject>Human T-lymphotropic virus 1 - isolation & purification</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - virology</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Mycosis Fungoides - blood</subject><subject>Mycosis Fungoides - virology</subject><subject>Polymerase Chain Reaction</subject><subject>Skin Neoplasms - blood</subject><subject>Skin Neoplasms - virology</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKtvClRuST4geEjzx_wtSVQFFWgkOC1cr8dqNqyQOdlK0n4MvjJeuKjhxmsO89_Rmfgi9AlIDUezdnRtrUKTmNWhJn6ANcEYqzal6ijaENE0FhMjn6DznO0KAMS7O0JnUgjFJN-jXV5dyyIubrMPR434d2wnvsHXDgIfDOPdxSXEOFt-HtGa8HGaHAb-92W2_V3CJs_uxHr0ZhwnPLoW5d6kdcDfEuMdjnOK02sG16U9ixj7FEc_tEty0ZPwzLD0eDzaWCtiv020Me5dfoGe-HbJ7eZoX6NvHD7vrm2r75dPn66ttZRmIpeJS70EwyTvOwHluifeMdp1WlGqndNcRZxXXSjegGJG-5RwE942kEhrq6QV6_5A7r93o9rZUKtXNnMLYpoOJbTD_bqbQm9t4bxrQIBtVAt6cAlIsb8iLGUM-3tlOLq7ZSKGaBgT9rxCEFEQKUYT1g9CmmHNy_rENEHPkbQpvU3gbbo68i-H13zc8yk-A6W9Gjqlb</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Manca, N</creator><creator>Piacentini, E</creator><creator>Gelmi, M</creator><creator>Calzavara, P</creator><creator>Manganoni, M A</creator><creator>Glukhov, A</creator><creator>Gargiulo, F</creator><creator>De Francesco, M</creator><creator>Pirali, F</creator><creator>De Panfilis, G</creator><creator>Turano, A</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19941101</creationdate><title>Persistence of human T cell lymphotropic virus type 1 (HTLV-1) sequences in peripheral blood mononuclear cells from patients with mycosis fungoides</title><author>Manca, N ; Piacentini, E ; Gelmi, M ; Calzavara, P ; Manganoni, M A ; Glukhov, A ; Gargiulo, F ; De Francesco, M ; Pirali, F ; De Panfilis, G ; Turano, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-579d16475b541ef5c0ff43bb98339e89bb0ec85989218407fa55165f2737123f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>AIDS/HIV</topic><topic>Base Sequence</topic><topic>Cells, Cultured</topic><topic>DNA, Viral - analysis</topic><topic>Genes, pX</topic><topic>Herpesvirus 4, Human - genetics</topic><topic>Herpesvirus 4, Human - isolation & purification</topic><topic>Human T-lymphotropic virus 1 - genetics</topic><topic>Human T-lymphotropic virus 1 - isolation & purification</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - virology</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Mycosis Fungoides - blood</topic><topic>Mycosis Fungoides - virology</topic><topic>Polymerase Chain Reaction</topic><topic>Skin Neoplasms - blood</topic><topic>Skin Neoplasms - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manca, N</creatorcontrib><creatorcontrib>Piacentini, E</creatorcontrib><creatorcontrib>Gelmi, M</creatorcontrib><creatorcontrib>Calzavara, P</creatorcontrib><creatorcontrib>Manganoni, M A</creatorcontrib><creatorcontrib>Glukhov, A</creatorcontrib><creatorcontrib>Gargiulo, F</creatorcontrib><creatorcontrib>De Francesco, M</creatorcontrib><creatorcontrib>Pirali, F</creatorcontrib><creatorcontrib>De Panfilis, G</creatorcontrib><creatorcontrib>Turano, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manca, N</au><au>Piacentini, E</au><au>Gelmi, M</au><au>Calzavara, P</au><au>Manganoni, M A</au><au>Glukhov, A</au><au>Gargiulo, F</au><au>De Francesco, M</au><au>Pirali, F</au><au>De Panfilis, G</au><au>Turano, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Persistence of human T cell lymphotropic virus type 1 (HTLV-1) sequences in peripheral blood mononuclear cells from patients with mycosis fungoides</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>180</volume><issue>5</issue><spage>1973</spage><epage>1978</epage><pages>1973-1978</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>Mycosis fungoides (MF) is a rare form of cutaneous T cell lymphoma suspected of having a viral etiology. As in adult T cell leukemia, the virus involved may be human T lymphotropic virus type 1 (HTLV-1). We cultured the peripheral blood mononuclear cells (PBMC) of 29 patients with MF HTLV-1 seronegative by enzyme-linked immunosorbent assay and Western blot. The presence of reverse transcriptase (RT) and p24 antigen was investigated in the concentrate supernatant of the culture. The DNA of all studied patients was submitted to polymerase chain reaction and Southern blot analysis using primers and probes recognizing the tax region of HTLV-1/2 and the pol region of HTLV-1. 10 of 29 patients were found positive to HTLV-1, whereas they were always negative to RT and p24. The same results were confirmed in double blind after 6 mo. 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| issn | 0022-1007 1540-9538 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Aged Aged, 80 and over AIDS/HIV Base Sequence Cells, Cultured DNA, Viral - analysis Genes, pX Herpesvirus 4, Human - genetics Herpesvirus 4, Human - isolation & purification Human T-lymphotropic virus 1 - genetics Human T-lymphotropic virus 1 - isolation & purification Humans Leukocytes, Mononuclear - virology Middle Aged Molecular Sequence Data Mycosis Fungoides - blood Mycosis Fungoides - virology Polymerase Chain Reaction Skin Neoplasms - blood Skin Neoplasms - virology |
| title | Persistence of human T cell lymphotropic virus type 1 (HTLV-1) sequences in peripheral blood mononuclear cells from patients with mycosis fungoides |
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