The pinocytic rate of activated macrophages
Peritoneal macrophages from mice injected 4 days previously with Brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (HRP)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng HRP/100 mug cell protein/h. Mice injected...
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| Veröffentlicht in: | The Journal of experimental medicine 1975-11, Vol.142 (5), p.1150-1164 |
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| container_title | The Journal of experimental medicine |
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| creator | Edelson, P J Zwiebel, R Cohn, Z A |
| description | Peritoneal macrophages from mice injected 4 days previously with Brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (HRP)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng HRP/100 mug cell protein/h. Mice injected with endotoxin or with only certain of the components of the Brewer's medium show an intermediate level of stimulation. The rate of unstimulated, endotoxin-stimulated, or thioglycollate-stimulated cells shows little change over several days in culture. The pinocytic rate of thioglycollate-stimulated cells can, however, be further increased by exposure of concanavalin A. Although cells may show transient increases in their pinocytic rate in many situations, a sustained increase in pinocytic rate is a sign of the "activated" state of macrophages. |
| doi_str_mv | 10.1084/jem.142.5.1150 |
| format | Article |
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Mice injected with endotoxin or with only certain of the components of the Brewer's medium show an intermediate level of stimulation. The rate of unstimulated, endotoxin-stimulated, or thioglycollate-stimulated cells shows little change over several days in culture. The pinocytic rate of thioglycollate-stimulated cells can, however, be further increased by exposure of concanavalin A. 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Mice injected with endotoxin or with only certain of the components of the Brewer's medium show an intermediate level of stimulation. The rate of unstimulated, endotoxin-stimulated, or thioglycollate-stimulated cells shows little change over several days in culture. The pinocytic rate of thioglycollate-stimulated cells can, however, be further increased by exposure of concanavalin A. Although cells may show transient increases in their pinocytic rate in many situations, a sustained increase in pinocytic rate is a sign of the "activated" state of macrophages.</description><subject>Animals</subject><subject>Ascitic Fluid - cytology</subject><subject>Cell Count</subject><subject>Cells, Cultured</subject><subject>Endotoxins - immunology</subject><subject>Female</subject><subject>Horseradish Peroxidase</subject><subject>Macrophages - immunology</subject><subject>Macrophages - ultrastructure</subject><subject>Male</subject><subject>Mice</subject><subject>Pinocytosis</subject><subject>Staining and Labeling</subject><subject>Thioglycolates - immunology</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1Lw0AQxRexaK1evXjJXRJndme6yUWQUj-g4CX3ZbvZtClNE5JY6H_v1krRuczAzHtv-Alxj5AgpPS08XWCJBNOEBkuxBiZIM5YpZdiDCBljAD6Wtz0_QYAiXh6JUasJKdj8ZivfdRWu8YdhspFnR181JSRdUO1D3MR1dZ1Tbu2K9_filFpt72_--0Tkb_O89l7vPh8-5i9LGKnWA4xawWKNC5RZ1RKVlNPzCSVTZXWEsixUwCQOqkKl8mMCk2IU1LahlIT8Xyybb-WtS-c3w2d3Zq2q2rbHUxjK_N_s6vWZtXsjcQ0yzQEg-RkED7v-86XZy2COSIzAZkJyAybI7IgePibeD7_YaS-AdaDZhA</recordid><startdate>19751101</startdate><enddate>19751101</enddate><creator>Edelson, P J</creator><creator>Zwiebel, R</creator><creator>Cohn, Z A</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19751101</creationdate><title>The pinocytic rate of activated macrophages</title><author>Edelson, P J ; Zwiebel, R ; Cohn, Z A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-57303471b1794f2536e455423a8377204c5c30008c23dc9294d74116437aaaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Ascitic Fluid - cytology</topic><topic>Cell Count</topic><topic>Cells, Cultured</topic><topic>Endotoxins - immunology</topic><topic>Female</topic><topic>Horseradish Peroxidase</topic><topic>Macrophages - immunology</topic><topic>Macrophages - ultrastructure</topic><topic>Male</topic><topic>Mice</topic><topic>Pinocytosis</topic><topic>Staining and Labeling</topic><topic>Thioglycolates - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Edelson, P J</creatorcontrib><creatorcontrib>Zwiebel, R</creatorcontrib><creatorcontrib>Cohn, Z A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Edelson, P J</au><au>Zwiebel, R</au><au>Cohn, Z A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The pinocytic rate of activated macrophages</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>1975-11-01</date><risdate>1975</risdate><volume>142</volume><issue>5</issue><spage>1150</spage><epage>1164</epage><pages>1150-1164</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>Peritoneal macrophages from mice injected 4 days previously with Brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (HRP)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng HRP/100 mug cell protein/h. Mice injected with endotoxin or with only certain of the components of the Brewer's medium show an intermediate level of stimulation. The rate of unstimulated, endotoxin-stimulated, or thioglycollate-stimulated cells shows little change over several days in culture. The pinocytic rate of thioglycollate-stimulated cells can, however, be further increased by exposure of concanavalin A. Although cells may show transient increases in their pinocytic rate in many situations, a sustained increase in pinocytic rate is a sign of the "activated" state of macrophages.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>53258</pmid><doi>10.1084/jem.142.5.1150</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Ascitic Fluid - cytology Cell Count Cells, Cultured Endotoxins - immunology Female Horseradish Peroxidase Macrophages - immunology Macrophages - ultrastructure Male Mice Pinocytosis Staining and Labeling Thioglycolates - immunology |
| title | The pinocytic rate of activated macrophages |
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