Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan
Antibodies, found in human sera from patients with poststreptococcal glomerulonephritis, against proteoglycans (PG) derived from bovine and human glomeruli were investigated. PG were isolated by 4 M guanidine-HCl extraction of whole glomeruli, followed by DEAE-Sepharose CL-6B ion exchange chromatogr...
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| Veröffentlicht in: | The Journal of experimental medicine 1985-02, Vol.161 (2), p.277-289 |
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| creator | FILLIT, H DAMLE, S. P GREGORY, J. D VOLIN, C POON-KING, T ZABRISKIE, J |
| description | Antibodies, found in human sera from patients with poststreptococcal glomerulonephritis, against proteoglycans (PG) derived from bovine and human glomeruli were investigated. PG were isolated by 4 M guanidine-HCl extraction of whole glomeruli, followed by DEAE-Sepharose CL-6B ion exchange chromatography. The anionic fractions were further purified by chromatography on Sepharose CL-4B. Biochemical analysis of the two resulting peaks revealed the presence of high molecular weight anionic material containing protein, uronic acid, glucosamine, and galactosamine. Enzymatic and chemical susceptibilities indicated the presence of heparan sulfate PG and a galactosamine-containing PG. Immunologic studies revealed the presence of anti-PG antibodies to both PG peaks of the Sepharose CL-4B column in glomerulonephritis sera. Inhibition studies using an ELISA demonstrated that heparan sulfate was a major antigenic determinant. Cross-reactivity with both mammalian and streptococcal hyaluronate was noted. Inhibition studies also indicated the presence of a second antigenic site containing N-acetylgalactosamine, possibly representing chondroitin or dermatan sulfate PG. |
| doi_str_mv | 10.1084/jem.161.2.277 |
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P ; GREGORY, J. D ; VOLIN, C ; POON-KING, T ; ZABRISKIE, J</creator><creatorcontrib>FILLIT, H ; DAMLE, S. P ; GREGORY, J. D ; VOLIN, C ; POON-KING, T ; ZABRISKIE, J</creatorcontrib><description>Antibodies, found in human sera from patients with poststreptococcal glomerulonephritis, against proteoglycans (PG) derived from bovine and human glomeruli were investigated. PG were isolated by 4 M guanidine-HCl extraction of whole glomeruli, followed by DEAE-Sepharose CL-6B ion exchange chromatography. The anionic fractions were further purified by chromatography on Sepharose CL-4B. Biochemical analysis of the two resulting peaks revealed the presence of high molecular weight anionic material containing protein, uronic acid, glucosamine, and galactosamine. Enzymatic and chemical susceptibilities indicated the presence of heparan sulfate PG and a galactosamine-containing PG. Immunologic studies revealed the presence of anti-PG antibodies to both PG peaks of the Sepharose CL-4B column in glomerulonephritis sera. Inhibition studies using an ELISA demonstrated that heparan sulfate was a major antigenic determinant. Cross-reactivity with both mammalian and streptococcal hyaluronate was noted. Inhibition studies also indicated the presence of a second antigenic site containing N-acetylgalactosamine, possibly representing chondroitin or dermatan sulfate PG.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.161.2.277</identifier><identifier>PMID: 3156204</identifier><identifier>CODEN: JEMEAV</identifier><language>eng</language><publisher>New York, NY: Rockefeller University Press</publisher><subject>Acute Disease ; Animals ; Antigen-Antibody Reactions ; Autoantibodies - analysis ; Biological and medical sciences ; Cattle ; Chemical Phenomena ; Chemistry, Physical ; Chondroitin Sulfate Proteoglycans - analysis ; Chondroitin Sulfate Proteoglycans - immunology ; Chondroitin Sulfate Proteoglycans - isolation & purification ; Enzyme-Linked Immunosorbent Assay ; Glomerulonephritis ; Glomerulonephritis - etiology ; Glomerulonephritis - immunology ; Glycosaminoglycans - immunology ; Heparan Sulfate Proteoglycans ; Heparitin Sulfate - analysis ; Heparitin Sulfate - immunology ; Heparitin Sulfate - isolation & purification ; Hexosamines - isolation & purification ; Humans ; Kidney Glomerulus - immunology ; Medical sciences ; Nephrology. Urinary tract diseases ; Nephropathies. Renovascular diseases. Renal failure ; Proteins - isolation & purification ; Proteoglycans - immunology ; Rabbits ; Streptococcal Infections - immunology ; Uronic Acids - isolation & purification</subject><ispartof>The Journal of experimental medicine, 1985-02, Vol.161 (2), p.277-289</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-6951377af6d9b79d79a4b16535444a8e8433533c594a46770bc7a06deb43552a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9218577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3156204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FILLIT, H</creatorcontrib><creatorcontrib>DAMLE, S. P</creatorcontrib><creatorcontrib>GREGORY, J. D</creatorcontrib><creatorcontrib>VOLIN, C</creatorcontrib><creatorcontrib>POON-KING, T</creatorcontrib><creatorcontrib>ZABRISKIE, J</creatorcontrib><title>Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>Antibodies, found in human sera from patients with poststreptococcal glomerulonephritis, against proteoglycans (PG) derived from bovine and human glomeruli were investigated. PG were isolated by 4 M guanidine-HCl extraction of whole glomeruli, followed by DEAE-Sepharose CL-6B ion exchange chromatography. The anionic fractions were further purified by chromatography on Sepharose CL-4B. Biochemical analysis of the two resulting peaks revealed the presence of high molecular weight anionic material containing protein, uronic acid, glucosamine, and galactosamine. Enzymatic and chemical susceptibilities indicated the presence of heparan sulfate PG and a galactosamine-containing PG. Immunologic studies revealed the presence of anti-PG antibodies to both PG peaks of the Sepharose CL-4B column in glomerulonephritis sera. Inhibition studies using an ELISA demonstrated that heparan sulfate was a major antigenic determinant. Cross-reactivity with both mammalian and streptococcal hyaluronate was noted. Inhibition studies also indicated the presence of a second antigenic site containing N-acetylgalactosamine, possibly representing chondroitin or dermatan sulfate PG.</description><subject>Acute Disease</subject><subject>Animals</subject><subject>Antigen-Antibody Reactions</subject><subject>Autoantibodies - analysis</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Chondroitin Sulfate Proteoglycans - analysis</subject><subject>Chondroitin Sulfate Proteoglycans - immunology</subject><subject>Chondroitin Sulfate Proteoglycans - isolation & purification</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Glomerulonephritis</subject><subject>Glomerulonephritis - etiology</subject><subject>Glomerulonephritis - immunology</subject><subject>Glycosaminoglycans - immunology</subject><subject>Heparan Sulfate Proteoglycans</subject><subject>Heparitin Sulfate - analysis</subject><subject>Heparitin Sulfate - immunology</subject><subject>Heparitin Sulfate - isolation & purification</subject><subject>Hexosamines - isolation & purification</subject><subject>Humans</subject><subject>Kidney Glomerulus - immunology</subject><subject>Medical sciences</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Nephropathies. Renovascular diseases. Renal failure</subject><subject>Proteins - isolation & purification</subject><subject>Proteoglycans - immunology</subject><subject>Rabbits</subject><subject>Streptococcal Infections - immunology</subject><subject>Uronic Acids - isolation & purification</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU2LFDEQhoMo6zh69CjkIN56TDpf3RdBFr9gwYN6DtXp6pks6U6bpF3WX29kh0FPObwPb1XqIeQlZwfOOvn2FucD1_zQHlpjHpEdV5I1vRLdY7JjrG0bzph5Sp7lfMsYl1LpK3IluNItkzvy-xsmoFOKM12heFxKpne-nOgac8kl4Vqii85BoMcQZ0xbiAuup-SLz9TFpYBfKCzFD3H0mGmJFxASPeEKCRaatzBBQbqmWDAew72D5Tl5MkHI-OL87smPjx--X39ubr5--nL9_qZxkvPS6F5xYQxMeuwH04-mBzlwrYSSUkKHnRRCCeFUL0FqY9jgDDA94iCFUi2IPXn30Ltuw4yjq39MEOya_Azp3kbw9v9k8Sd7jL9syzuj6qA9eXMuSPHnhrnY2WeHIcCCccvWaMYk73gFmwfQpZhzwukyhDP7V5atsmyVZVtbZVX-1b-bXeiznZq_PueQq4GpXtL5fMH6uqCqNX8AY8qhRQ</recordid><startdate>19850201</startdate><enddate>19850201</enddate><creator>FILLIT, H</creator><creator>DAMLE, S. P</creator><creator>GREGORY, J. D</creator><creator>VOLIN, C</creator><creator>POON-KING, T</creator><creator>ZABRISKIE, J</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850201</creationdate><title>Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan</title><author>FILLIT, H ; DAMLE, S. P ; GREGORY, J. D ; VOLIN, C ; POON-KING, T ; ZABRISKIE, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-6951377af6d9b79d79a4b16535444a8e8433533c594a46770bc7a06deb43552a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Acute Disease</topic><topic>Animals</topic><topic>Antigen-Antibody Reactions</topic><topic>Autoantibodies - analysis</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Chondroitin Sulfate Proteoglycans - analysis</topic><topic>Chondroitin Sulfate Proteoglycans - immunology</topic><topic>Chondroitin Sulfate Proteoglycans - isolation & purification</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Glomerulonephritis</topic><topic>Glomerulonephritis - etiology</topic><topic>Glomerulonephritis - immunology</topic><topic>Glycosaminoglycans - immunology</topic><topic>Heparan Sulfate Proteoglycans</topic><topic>Heparitin Sulfate - analysis</topic><topic>Heparitin Sulfate - immunology</topic><topic>Heparitin Sulfate - isolation & purification</topic><topic>Hexosamines - isolation & purification</topic><topic>Humans</topic><topic>Kidney Glomerulus - immunology</topic><topic>Medical sciences</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Nephropathies. Renovascular diseases. Renal failure</topic><topic>Proteins - isolation & purification</topic><topic>Proteoglycans - immunology</topic><topic>Rabbits</topic><topic>Streptococcal Infections - immunology</topic><topic>Uronic Acids - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FILLIT, H</creatorcontrib><creatorcontrib>DAMLE, S. P</creatorcontrib><creatorcontrib>GREGORY, J. D</creatorcontrib><creatorcontrib>VOLIN, C</creatorcontrib><creatorcontrib>POON-KING, T</creatorcontrib><creatorcontrib>ZABRISKIE, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FILLIT, H</au><au>DAMLE, S. P</au><au>GREGORY, J. D</au><au>VOLIN, C</au><au>POON-KING, T</au><au>ZABRISKIE, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>1985-02-01</date><risdate>1985</risdate><volume>161</volume><issue>2</issue><spage>277</spage><epage>289</epage><pages>277-289</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><coden>JEMEAV</coden><abstract>Antibodies, found in human sera from patients with poststreptococcal glomerulonephritis, against proteoglycans (PG) derived from bovine and human glomeruli were investigated. PG were isolated by 4 M guanidine-HCl extraction of whole glomeruli, followed by DEAE-Sepharose CL-6B ion exchange chromatography. The anionic fractions were further purified by chromatography on Sepharose CL-4B. Biochemical analysis of the two resulting peaks revealed the presence of high molecular weight anionic material containing protein, uronic acid, glucosamine, and galactosamine. Enzymatic and chemical susceptibilities indicated the presence of heparan sulfate PG and a galactosamine-containing PG. Immunologic studies revealed the presence of anti-PG antibodies to both PG peaks of the Sepharose CL-4B column in glomerulonephritis sera. Inhibition studies using an ELISA demonstrated that heparan sulfate was a major antigenic determinant. Cross-reactivity with both mammalian and streptococcal hyaluronate was noted. Inhibition studies also indicated the presence of a second antigenic site containing N-acetylgalactosamine, possibly representing chondroitin or dermatan sulfate PG.</abstract><cop>New York, NY</cop><pub>Rockefeller University Press</pub><pmid>3156204</pmid><doi>10.1084/jem.161.2.277</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of experimental medicine, 1985-02, Vol.161 (2), p.277-289 |
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| subjects | Acute Disease Animals Antigen-Antibody Reactions Autoantibodies - analysis Biological and medical sciences Cattle Chemical Phenomena Chemistry, Physical Chondroitin Sulfate Proteoglycans - analysis Chondroitin Sulfate Proteoglycans - immunology Chondroitin Sulfate Proteoglycans - isolation & purification Enzyme-Linked Immunosorbent Assay Glomerulonephritis Glomerulonephritis - etiology Glomerulonephritis - immunology Glycosaminoglycans - immunology Heparan Sulfate Proteoglycans Heparitin Sulfate - analysis Heparitin Sulfate - immunology Heparitin Sulfate - isolation & purification Hexosamines - isolation & purification Humans Kidney Glomerulus - immunology Medical sciences Nephrology. Urinary tract diseases Nephropathies. Renovascular diseases. Renal failure Proteins - isolation & purification Proteoglycans - immunology Rabbits Streptococcal Infections - immunology Uronic Acids - isolation & purification |
| title | Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan |
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