Generation of cytolytic T lymphocytes in thymectomized, irradiated, and bone marrow-reconstituted mice
A model system has been developed to study extrathymic T cell differentiation. Mice have been thymectomized, lethally irradiated, and reconstituted with bone marrow cells depleted of Thy-1-positive cells. After 8 wk, the spleen cells of these 5athymic, bone marrow-reconstituted chimeras contain Thy-...
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| Veröffentlicht in: | The Journal of experimental medicine 1982-09, Vol.156 (3), p.844-859 |
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| container_title | The Journal of experimental medicine |
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| creator | Duprez, V Hamilton, B Burakoff, S J |
| description | A model system has been developed to study extrathymic T cell differentiation. Mice have been thymectomized, lethally irradiated, and reconstituted with bone marrow cells depleted of Thy-1-positive cells. After 8 wk, the spleen cells of these 5athymic, bone marrow-reconstituted chimeras contain Thy-1-positive pre-cytolytic T lymphocytes (CTL) that are able to respond to antigen only when exogenous interleukin 2 is added to culture.. The phenotype of these pre-CTL is similar to that of thymocytes, suggesting that they may be an immature T cell. Initial evaluation of the CTL repertoire of these athymic mice demonstrates that the CTL generated to trinitrophenyl-modified syngeneic cells are H-2 restricted and that the CTL generated to alloantigens have many of the cross-reactivities observed in normal but not in nude mice. The discrepancies observed in the CTL repertoire between these thymectomized chimeras and nude mice are discussed. |
| doi_str_mv | 10.1084/jem.156.3.844 |
| format | Article |
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Mice have been thymectomized, lethally irradiated, and reconstituted with bone marrow cells depleted of Thy-1-positive cells. After 8 wk, the spleen cells of these 5athymic, bone marrow-reconstituted chimeras contain Thy-1-positive pre-cytolytic T lymphocytes (CTL) that are able to respond to antigen only when exogenous interleukin 2 is added to culture.. The phenotype of these pre-CTL is similar to that of thymocytes, suggesting that they may be an immature T cell. Initial evaluation of the CTL repertoire of these athymic mice demonstrates that the CTL generated to trinitrophenyl-modified syngeneic cells are H-2 restricted and that the CTL generated to alloantigens have many of the cross-reactivities observed in normal but not in nude mice. The discrepancies observed in the CTL repertoire between these thymectomized chimeras and nude mice are discussed.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.156.3.844</identifier><identifier>PMID: 6125557</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Animals ; Antigens, Ly ; Antigens, Surface ; Bone Marrow Transplantation ; Chimera ; Cross Reactions ; Cytotoxicity, Immunologic ; H-2 Antigens ; Interleukin-2 - pharmacology ; Major Histocompatibility Complex ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; T-Lymphocytes - immunology ; Thy-1 Antigens ; Thymectomy</subject><ispartof>The Journal of experimental medicine, 1982-09, Vol.156 (3), p.844-859</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3574-7d0ee5f177021d9de1e1ce02b8c4650e816ab31bb5761afd6b2c7ffdb2b8f813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6125557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duprez, V</creatorcontrib><creatorcontrib>Hamilton, B</creatorcontrib><creatorcontrib>Burakoff, S J</creatorcontrib><title>Generation of cytolytic T lymphocytes in thymectomized, irradiated, and bone marrow-reconstituted mice</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>A model system has been developed to study extrathymic T cell differentiation. Mice have been thymectomized, lethally irradiated, and reconstituted with bone marrow cells depleted of Thy-1-positive cells. After 8 wk, the spleen cells of these 5athymic, bone marrow-reconstituted chimeras contain Thy-1-positive pre-cytolytic T lymphocytes (CTL) that are able to respond to antigen only when exogenous interleukin 2 is added to culture.. The phenotype of these pre-CTL is similar to that of thymocytes, suggesting that they may be an immature T cell. Initial evaluation of the CTL repertoire of these athymic mice demonstrates that the CTL generated to trinitrophenyl-modified syngeneic cells are H-2 restricted and that the CTL generated to alloantigens have many of the cross-reactivities observed in normal but not in nude mice. The discrepancies observed in the CTL repertoire between these thymectomized chimeras and nude mice are discussed.</description><subject>Animals</subject><subject>Antigens, Ly</subject><subject>Antigens, Surface</subject><subject>Bone Marrow Transplantation</subject><subject>Chimera</subject><subject>Cross Reactions</subject><subject>Cytotoxicity, Immunologic</subject><subject>H-2 Antigens</subject><subject>Interleukin-2 - pharmacology</subject><subject>Major Histocompatibility Complex</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>T-Lymphocytes - immunology</subject><subject>Thy-1 Antigens</subject><subject>Thymectomy</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo67h69Cjk5Gl7Nknno_siyOKuwsJe5h7SScXJ0p2MSUZpf70Zdlj05KmKqoeXKh6E3lOypWTg14-wbKmQ2347cP4CbajgpBtFP7xEG0IY6ygh6jV6U8ojIZRzIS_QhaRMCKE2yN9BhGxqSBEnj-1a07zWYPEOz-ty2Kc2gYJDxHW_LmBrWsJvcFc45GxcMPXUm-jwlCLgxeScfnUZbIqlhnpsa7wEC2_RK2_mAu_O9RLtbr_sbr529w93324-33e2F4p3yhEA4alShFE3OqBALRA2DZZLQWCg0kw9nSahJDXeyYlZ5b2bGuEH2l-iT0-xh-O0gLMQazazPuTQLlt1MkH_u4lhr7-nn5rRQapxbAEfzwE5_ThCqXoJxcI8mwjpWLTijDAl2X_BZmEUYjiB3RNocyolg3--hhJ9EqibwIZL3esmsPEf_n7hmT4b6_8Ai02aZw</recordid><startdate>19820901</startdate><enddate>19820901</enddate><creator>Duprez, V</creator><creator>Hamilton, B</creator><creator>Burakoff, S J</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19820901</creationdate><title>Generation of cytolytic T lymphocytes in thymectomized, irradiated, and bone marrow-reconstituted mice</title><author>Duprez, V ; Hamilton, B ; Burakoff, S J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3574-7d0ee5f177021d9de1e1ce02b8c4650e816ab31bb5761afd6b2c7ffdb2b8f813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Antigens, Ly</topic><topic>Antigens, Surface</topic><topic>Bone Marrow Transplantation</topic><topic>Chimera</topic><topic>Cross Reactions</topic><topic>Cytotoxicity, Immunologic</topic><topic>H-2 Antigens</topic><topic>Interleukin-2 - pharmacology</topic><topic>Major Histocompatibility Complex</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>T-Lymphocytes - immunology</topic><topic>Thy-1 Antigens</topic><topic>Thymectomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duprez, V</creatorcontrib><creatorcontrib>Hamilton, B</creatorcontrib><creatorcontrib>Burakoff, S J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duprez, V</au><au>Hamilton, B</au><au>Burakoff, S J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of cytolytic T lymphocytes in thymectomized, irradiated, and bone marrow-reconstituted mice</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>1982-09-01</date><risdate>1982</risdate><volume>156</volume><issue>3</issue><spage>844</spage><epage>859</epage><pages>844-859</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>A model system has been developed to study extrathymic T cell differentiation. Mice have been thymectomized, lethally irradiated, and reconstituted with bone marrow cells depleted of Thy-1-positive cells. After 8 wk, the spleen cells of these 5athymic, bone marrow-reconstituted chimeras contain Thy-1-positive pre-cytolytic T lymphocytes (CTL) that are able to respond to antigen only when exogenous interleukin 2 is added to culture.. The phenotype of these pre-CTL is similar to that of thymocytes, suggesting that they may be an immature T cell. Initial evaluation of the CTL repertoire of these athymic mice demonstrates that the CTL generated to trinitrophenyl-modified syngeneic cells are H-2 restricted and that the CTL generated to alloantigens have many of the cross-reactivities observed in normal but not in nude mice. The discrepancies observed in the CTL repertoire between these thymectomized chimeras and nude mice are discussed.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>6125557</pmid><doi>10.1084/jem.156.3.844</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1007 |
| ispartof | The Journal of experimental medicine, 1982-09, Vol.156 (3), p.844-859 |
| issn | 0022-1007 1540-9538 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antigens, Ly Antigens, Surface Bone Marrow Transplantation Chimera Cross Reactions Cytotoxicity, Immunologic H-2 Antigens Interleukin-2 - pharmacology Major Histocompatibility Complex Mice Mice, Inbred BALB C Mice, Inbred C57BL T-Lymphocytes - immunology Thy-1 Antigens Thymectomy |
| title | Generation of cytolytic T lymphocytes in thymectomized, irradiated, and bone marrow-reconstituted mice |
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