Helper T cells are required for the polyclonal stimulation of cytotoxic T cells by concanavalin A
Concanavalin A stimulation of T-cell cytotoxicity has been shown to be absolutely dependent on helper T-cell collaboration. Thymocytes stimulated with ConA do not differentiate to yield cytotoxic effector cells. However, thymocytes cocultured with irradiated spleen cells as helpers and ConA yield hi...
Gespeichert in:
| Veröffentlicht in: | The Journal of experimental medicine 1977-05, Vol.145 (5), p.1237-1249 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1249 |
|---|---|
| container_issue | 5 |
| container_start_page | 1237 |
| container_title | The Journal of experimental medicine |
| container_volume | 145 |
| creator | Pilarski, L M Bretscher, P A Baum, L L |
| description | Concanavalin A stimulation of T-cell cytotoxicity has been shown to be absolutely dependent on helper T-cell collaboration. Thymocytes stimulated with ConA do not differentiate to yield cytotoxic effector cells. However, thymocytes cocultured with irradiated spleen cells as helpers and ConA yield high levels of cytotoxicity. The helper cell bears theta antigens on its surface, is not an adherent cell, and does not require any adherent cell functions in our culture conditions. The ConA-dependent helper cells appear to be polyclonal in specificity. Thus, polyclonal stimulation of cytotoxicity by ConA requires T helper-T precursor collaboration in analogy to antigen-specific T helper-T precursor interactions. Unlike the antigen-specific interacitons, the ConA-driven cytotoxicity does not appear to require linked associative recognition for induction of cytotoxicity. |
| doi_str_mv | 10.1084/jem.145.5.1237 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2180654</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>300781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-995bc82b52329a0c91068b990f021e75e21bfc63a4d40d53908c660dec2629603</originalsourceid><addsrcrecordid>eNpVkMtOwzAQRS3EqxS2rFj4BxLGrzTeIFUVUKRKbMrachyHunLi4KQV-XtSFVWwGmnm3jPSQeieQEog549bW6eEi1SkhLLZGZoQwSGRguXnaAJAaUIAZtfopuu2AIRzkV2hSzbucjJBeml9ayNeY2O977COFkf7tXPRlrgKEfcbi9vgB-NDoz3uelfvvO5daHCosBn60IdvZ06AYsAmNEY3eq-9a_D8Fl1U2nf27ndO0cfL83qxTFbvr2-L-SoxLOd9IqUoTE4LQRmVGowkkOWFlFABJXYmLCVFZTKmecmhFExCbrIMSmtoRmUGbIqejtx2V9S2NLbpo_aqja7WcVBBO_X_0riN-gx7RUkOmeAjID0CTAxdF2116hJQB9VqVK1G1Uqog-qx8PD34yl-dMt-ADAve3g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Helper T cells are required for the polyclonal stimulation of cytotoxic T cells by concanavalin A</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Pilarski, L M ; Bretscher, P A ; Baum, L L</creator><creatorcontrib>Pilarski, L M ; Bretscher, P A ; Baum, L L</creatorcontrib><description>Concanavalin A stimulation of T-cell cytotoxicity has been shown to be absolutely dependent on helper T-cell collaboration. Thymocytes stimulated with ConA do not differentiate to yield cytotoxic effector cells. However, thymocytes cocultured with irradiated spleen cells as helpers and ConA yield high levels of cytotoxicity. The helper cell bears theta antigens on its surface, is not an adherent cell, and does not require any adherent cell functions in our culture conditions. The ConA-dependent helper cells appear to be polyclonal in specificity. Thus, polyclonal stimulation of cytotoxicity by ConA requires T helper-T precursor collaboration in analogy to antigen-specific T helper-T precursor interactions. Unlike the antigen-specific interacitons, the ConA-driven cytotoxicity does not appear to require linked associative recognition for induction of cytotoxicity.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.145.5.1237</identifier><identifier>PMID: 300781</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Animals ; Antibody-Producing Cells ; Cells, Cultured ; Clone Cells ; Concanavalin A - pharmacology ; Cytotoxicity Tests, Immunologic ; Dose-Response Relationship, Immunologic ; Lymphocyte Activation ; Mice ; Mice, Inbred BALB C ; Mice, Inbred CBA ; Mitogens ; Spleen - immunology ; T-Lymphocytes - immunology ; Thymus Gland - immunology ; Time Factors</subject><ispartof>The Journal of experimental medicine, 1977-05, Vol.145 (5), p.1237-1249</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-995bc82b52329a0c91068b990f021e75e21bfc63a4d40d53908c660dec2629603</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/300781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pilarski, L M</creatorcontrib><creatorcontrib>Bretscher, P A</creatorcontrib><creatorcontrib>Baum, L L</creatorcontrib><title>Helper T cells are required for the polyclonal stimulation of cytotoxic T cells by concanavalin A</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>Concanavalin A stimulation of T-cell cytotoxicity has been shown to be absolutely dependent on helper T-cell collaboration. Thymocytes stimulated with ConA do not differentiate to yield cytotoxic effector cells. However, thymocytes cocultured with irradiated spleen cells as helpers and ConA yield high levels of cytotoxicity. The helper cell bears theta antigens on its surface, is not an adherent cell, and does not require any adherent cell functions in our culture conditions. The ConA-dependent helper cells appear to be polyclonal in specificity. Thus, polyclonal stimulation of cytotoxicity by ConA requires T helper-T precursor collaboration in analogy to antigen-specific T helper-T precursor interactions. Unlike the antigen-specific interacitons, the ConA-driven cytotoxicity does not appear to require linked associative recognition for induction of cytotoxicity.</description><subject>Animals</subject><subject>Antibody-Producing Cells</subject><subject>Cells, Cultured</subject><subject>Clone Cells</subject><subject>Concanavalin A - pharmacology</subject><subject>Cytotoxicity Tests, Immunologic</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Lymphocyte Activation</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred CBA</subject><subject>Mitogens</subject><subject>Spleen - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>Thymus Gland - immunology</subject><subject>Time Factors</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtOwzAQRS3EqxS2rFj4BxLGrzTeIFUVUKRKbMrachyHunLi4KQV-XtSFVWwGmnm3jPSQeieQEog549bW6eEi1SkhLLZGZoQwSGRguXnaAJAaUIAZtfopuu2AIRzkV2hSzbucjJBeml9ayNeY2O977COFkf7tXPRlrgKEfcbi9vgB-NDoz3uelfvvO5daHCosBn60IdvZ06AYsAmNEY3eq-9a_D8Fl1U2nf27ndO0cfL83qxTFbvr2-L-SoxLOd9IqUoTE4LQRmVGowkkOWFlFABJXYmLCVFZTKmecmhFExCbrIMSmtoRmUGbIqejtx2V9S2NLbpo_aqja7WcVBBO_X_0riN-gx7RUkOmeAjID0CTAxdF2116hJQB9VqVK1G1Uqog-qx8PD34yl-dMt-ADAve3g</recordid><startdate>19770501</startdate><enddate>19770501</enddate><creator>Pilarski, L M</creator><creator>Bretscher, P A</creator><creator>Baum, L L</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19770501</creationdate><title>Helper T cells are required for the polyclonal stimulation of cytotoxic T cells by concanavalin A</title><author>Pilarski, L M ; Bretscher, P A ; Baum, L L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-995bc82b52329a0c91068b990f021e75e21bfc63a4d40d53908c660dec2629603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Antibody-Producing Cells</topic><topic>Cells, Cultured</topic><topic>Clone Cells</topic><topic>Concanavalin A - pharmacology</topic><topic>Cytotoxicity Tests, Immunologic</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Lymphocyte Activation</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred CBA</topic><topic>Mitogens</topic><topic>Spleen - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>Thymus Gland - immunology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pilarski, L M</creatorcontrib><creatorcontrib>Bretscher, P A</creatorcontrib><creatorcontrib>Baum, L L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pilarski, L M</au><au>Bretscher, P A</au><au>Baum, L L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Helper T cells are required for the polyclonal stimulation of cytotoxic T cells by concanavalin A</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>1977-05-01</date><risdate>1977</risdate><volume>145</volume><issue>5</issue><spage>1237</spage><epage>1249</epage><pages>1237-1249</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>Concanavalin A stimulation of T-cell cytotoxicity has been shown to be absolutely dependent on helper T-cell collaboration. Thymocytes stimulated with ConA do not differentiate to yield cytotoxic effector cells. However, thymocytes cocultured with irradiated spleen cells as helpers and ConA yield high levels of cytotoxicity. The helper cell bears theta antigens on its surface, is not an adherent cell, and does not require any adherent cell functions in our culture conditions. The ConA-dependent helper cells appear to be polyclonal in specificity. Thus, polyclonal stimulation of cytotoxicity by ConA requires T helper-T precursor collaboration in analogy to antigen-specific T helper-T precursor interactions. Unlike the antigen-specific interacitons, the ConA-driven cytotoxicity does not appear to require linked associative recognition for induction of cytotoxicity.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>300781</pmid><doi>10.1084/jem.145.5.1237</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1007 |
| ispartof | The Journal of experimental medicine, 1977-05, Vol.145 (5), p.1237-1249 |
| issn | 0022-1007 1540-9538 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2180654 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibody-Producing Cells Cells, Cultured Clone Cells Concanavalin A - pharmacology Cytotoxicity Tests, Immunologic Dose-Response Relationship, Immunologic Lymphocyte Activation Mice Mice, Inbred BALB C Mice, Inbred CBA Mitogens Spleen - immunology T-Lymphocytes - immunology Thymus Gland - immunology Time Factors |
| title | Helper T cells are required for the polyclonal stimulation of cytotoxic T cells by concanavalin A |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T08%3A57%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Helper%20T%20cells%20are%20required%20for%20the%20polyclonal%20stimulation%20of%20cytotoxic%20T%20cells%20by%20concanavalin%20A&rft.jtitle=The%20Journal%20of%20experimental%20medicine&rft.au=Pilarski,%20L%20M&rft.date=1977-05-01&rft.volume=145&rft.issue=5&rft.spage=1237&rft.epage=1249&rft.pages=1237-1249&rft.issn=0022-1007&rft.eissn=1540-9538&rft_id=info:doi/10.1084/jem.145.5.1237&rft_dat=%3Cpubmed_cross%3E300781%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/300781&rfr_iscdi=true |