Peroxidase-mediated mammalian cell cytotoxicity

Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. Myeloperoxidase, in amounts equivalent to 1.5 x 10(6) neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. Th...

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Veröffentlicht in:	The Journal of experimental medicine 1973-07, Vol.138 (1), p.318-323
Hauptverfasser:	Edelson, P J, Cohn, Z A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brief Definitive Reports Cells, Cultured Chromium Isotopes Cytotoxicity Tests, Immunologic Erythrocytes - drug effects Erythrocytes - immunology Glucose Oxidase - pharmacology Iodides - pharmacology Isotope Labeling Leukemia L1210 Leukocytes - drug effects Leukocytes - immunology Methods Mice Peroxidases - pharmacology Trypan Blue
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container_title	The Journal of experimental medicine
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creator	Edelson, P J Cohn, Z A
description	Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. Myeloperoxidase, in amounts equivalent to 1.5 x 10(6) neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. The myeloperoxidase-mediated reaction is rapid, and highly efficient, leading to 85-90% cell death in 90 min, as measured by (51)chromium release and dye exclusion. The mixture of granulocytes. monocytes, and lymphocytes present in an inflammatory exudate, and the intimate cell-to-cell association characteristic of cytotoxic phenomena may provide the in vivo requirements for such a system.
doi_str_mv	10.1084/jem.138.1.318
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Myeloperoxidase, in amounts equivalent to 1.5 x 10(6) neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. The myeloperoxidase-mediated reaction is rapid, and highly efficient, leading to 85-90% cell death in 90 min, as measured by (51)chromium release and dye exclusion. The mixture of granulocytes. monocytes, and lymphocytes present in an inflammatory exudate, and the intimate cell-to-cell association characteristic of cytotoxic phenomena may provide the in vivo requirements for such a system.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.138.1.318</identifier><identifier>PMID: 4717124</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Animals ; Brief Definitive Reports ; Cells, Cultured ; Chromium Isotopes ; Cytotoxicity Tests, Immunologic ; Erythrocytes - drug effects ; Erythrocytes - immunology ; Glucose Oxidase - pharmacology ; Iodides - pharmacology ; Isotope Labeling ; Leukemia L1210 ; Leukocytes - drug effects ; Leukocytes - immunology ; Methods ; Mice ; Peroxidases - pharmacology ; Trypan Blue</subject><ispartof>The Journal of experimental medicine, 1973-07, Vol.138 (1), p.318-323</ispartof><rights>Copyright © 1973 by The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-9d0480dcd33e004baebb9b1c0bd7540f31adfb42bd4e916256b7c333b7b63bb33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4717124$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Edelson, P J</creatorcontrib><creatorcontrib>Cohn, Z A</creatorcontrib><title>Peroxidase-mediated mammalian cell cytotoxicity</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. Myeloperoxidase, in amounts equivalent to 1.5 x 10(6) neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. The myeloperoxidase-mediated reaction is rapid, and highly efficient, leading to 85-90% cell death in 90 min, as measured by (51)chromium release and dye exclusion. The mixture of granulocytes. monocytes, and lymphocytes present in an inflammatory exudate, and the intimate cell-to-cell association characteristic of cytotoxic phenomena may provide the in vivo requirements for such a system.</description><subject>Animals</subject><subject>Brief Definitive Reports</subject><subject>Cells, Cultured</subject><subject>Chromium Isotopes</subject><subject>Cytotoxicity Tests, Immunologic</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - immunology</subject><subject>Glucose Oxidase - pharmacology</subject><subject>Iodides - pharmacology</subject><subject>Isotope Labeling</subject><subject>Leukemia L1210</subject><subject>Leukocytes - drug effects</subject><subject>Leukocytes - immunology</subject><subject>Methods</subject><subject>Mice</subject><subject>Peroxidases - pharmacology</subject><subject>Trypan Blue</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1Lw0AQhhdRaq0ePQr9A5vOZDbZ5CJIsSoU9KDnZb-iW5KmJFHMv3elpehpDvPOMy8PY9cICUIhFhvfJEhFgglhccKmmAngZUbFKZsCpClHAHnOLvp-A4BCZPmETYREiamYssWL79rv4HTveeNd0IN380Y3ja6D3s6tr-u5HYd2iCEbhvGSnVW67v3VYc7Y2-r-dfnI188PT8u7NbdCyoGXDkQBzjoiDyCM9saUBi0YJ2O_ilC7yojUOOFLzNMsN9ISkZEmJ2OIZux2z919mtjL-u3Q6VrtutDoblStDur_Zhs-1Hv7pVIsIMswAvgeYLu27ztfHW8R1K84FcWpKE6hiuJi_ubvw2P6YIp-AFisa1c</recordid><startdate>19730701</startdate><enddate>19730701</enddate><creator>Edelson, P J</creator><creator>Cohn, Z A</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19730701</creationdate><title>Peroxidase-mediated mammalian cell cytotoxicity</title><author>Edelson, P J ; Cohn, Z A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-9d0480dcd33e004baebb9b1c0bd7540f31adfb42bd4e916256b7c333b7b63bb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Brief Definitive Reports</topic><topic>Cells, Cultured</topic><topic>Chromium Isotopes</topic><topic>Cytotoxicity Tests, Immunologic</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - immunology</topic><topic>Glucose Oxidase - pharmacology</topic><topic>Iodides - pharmacology</topic><topic>Isotope Labeling</topic><topic>Leukemia L1210</topic><topic>Leukocytes - drug effects</topic><topic>Leukocytes - immunology</topic><topic>Methods</topic><topic>Mice</topic><topic>Peroxidases - pharmacology</topic><topic>Trypan Blue</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Edelson, P J</creatorcontrib><creatorcontrib>Cohn, Z A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Edelson, P J</au><au>Cohn, Z A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peroxidase-mediated mammalian cell cytotoxicity</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>1973-07-01</date><risdate>1973</risdate><volume>138</volume><issue>1</issue><spage>318</spage><epage>323</epage><pages>318-323</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. 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subjects	Animals Brief Definitive Reports Cells, Cultured Chromium Isotopes Cytotoxicity Tests, Immunologic Erythrocytes - drug effects Erythrocytes - immunology Glucose Oxidase - pharmacology Iodides - pharmacology Isotope Labeling Leukemia L1210 Leukocytes - drug effects Leukocytes - immunology Methods Mice Peroxidases - pharmacology Trypan Blue
title	Peroxidase-mediated mammalian cell cytotoxicity
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