A Functional Link between Dynamin and the Actin Cytoskeleton at Podosomes
Cell transformation by Rous sarcoma virus results in a dramatic change of adhesion structures with the substratum. Adhesion plaques are replaced by dot-like attachment sites called podosomes. Podosomes are also found constitutively in motile nontransformed cells such as leukocytes, macrophages, and...
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| Veröffentlicht in: | The Journal of cell biology 2000-07, Vol.150 (2), p.377-389 |
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| creator | Ochoa, Gian-Carlo Slepnev, Vladimir I. Neff, Lynn Ringstad, Niels Takei, Kohji Daniell, Laurie Kim, Warren Cao, Hong McNiven, Mark Baron, Roland de Camilli, Pietro |
| description | Cell transformation by Rous sarcoma virus results in a dramatic change of adhesion structures with the substratum. Adhesion plaques are replaced by dot-like attachment sites called podosomes. Podosomes are also found constitutively in motile nontransformed cells such as leukocytes, macrophages, and osteoclasts. They are represented by columnar arrays of actin which are perpendicular to the substratum and contain tubular invaginations of the plasma membrane. Given the similarity of these tubules to those generated by dynamin around a variety of membrane templates, we investigated whether dynamin is present at podosomes. Immunoreactivities for dynamin 2 and for the dynamin 2-binding protein endophilin 2 (SH3P8) were detected at podosomes of transformed cells and osteoclasts. Furthermore, GFP wild-type dynamin 2aa was targeted to podosomes. As shown by fluorescence recovery after photobleaching, GFP-dynamin 2aa and GFP-actin had a very rapid and similar turnover at podosomes. Expression of the GFP-dynamin 2 aaG273 Dabolished podosomes while GPF-dynaminK44 Awas targeted to podosomes but delayed actin turnover. These data demonstrate a functional link between a member of the dynamin family and actin at attachment sites between cells and the substratum. |
| doi_str_mv | 10.1083/jcb.150.2.377 |
| format | Article |
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Adhesion plaques are replaced by dot-like attachment sites called podosomes. Podosomes are also found constitutively in motile nontransformed cells such as leukocytes, macrophages, and osteoclasts. They are represented by columnar arrays of actin which are perpendicular to the substratum and contain tubular invaginations of the plasma membrane. Given the similarity of these tubules to those generated by dynamin around a variety of membrane templates, we investigated whether dynamin is present at podosomes. Immunoreactivities for dynamin 2 and for the dynamin 2-binding protein endophilin 2 (SH3P8) were detected at podosomes of transformed cells and osteoclasts. Furthermore, GFP wild-type dynamin 2aa was targeted to podosomes. As shown by fluorescence recovery after photobleaching, GFP-dynamin 2aa and GFP-actin had a very rapid and similar turnover at podosomes. Expression of the GFP-dynamin 2 aaG273 Dabolished podosomes while GPF-dynaminK44 Awas targeted to podosomes but delayed actin turnover. These data demonstrate a functional link between a member of the dynamin family and actin at attachment sites between cells and the substratum.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.150.2.377</identifier><identifier>PMID: 10908579</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Actins ; Actins - metabolism ; Antibodies ; Body Temperature - physiology ; Cell Adhesion - drug effects ; Cell Adhesion - physiology ; Cell Line, Transformed - drug effects ; Cell Line, Transformed - metabolism ; Cell Line, Transformed - ultrastructure ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell Membrane - ultrastructure ; Cell membranes ; Cells ; Cellular biology ; Cellular immunity ; Cyclosporine - pharmacology ; Cyclosporins ; Cytoskeleton - drug effects ; Cytoskeleton - metabolism ; Cytoskeleton - ultrastructure ; Dynamin I ; Dynamins ; Endocytosis ; Fluorescence ; GTP Phosphohydrolases - metabolism ; Microtubules - drug effects ; Microtubules - metabolism ; Microtubules - ultrastructure ; Mutation - physiology ; Neurons ; Original ; Osteoclasts ; Osteoclasts - drug effects ; Osteoclasts - metabolism ; Osteoclasts - ultrastructure ; Viruses</subject><ispartof>The Journal of cell biology, 2000-07, Vol.150 (2), p.377-389</ispartof><rights>Copyright 2000 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Jul 24, 2000</rights><rights>2000 The Rockefeller University Press 2000 The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-e4d531352cacc6c29fada81b96d55896ea74ec17a4a84c5284fd677304efb8c93</citedby><cites>FETCH-LOGICAL-c497t-e4d531352cacc6c29fada81b96d55896ea74ec17a4a84c5284fd677304efb8c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10908579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ochoa, Gian-Carlo</creatorcontrib><creatorcontrib>Slepnev, Vladimir I.</creatorcontrib><creatorcontrib>Neff, Lynn</creatorcontrib><creatorcontrib>Ringstad, Niels</creatorcontrib><creatorcontrib>Takei, Kohji</creatorcontrib><creatorcontrib>Daniell, Laurie</creatorcontrib><creatorcontrib>Kim, Warren</creatorcontrib><creatorcontrib>Cao, Hong</creatorcontrib><creatorcontrib>McNiven, Mark</creatorcontrib><creatorcontrib>Baron, Roland</creatorcontrib><creatorcontrib>de Camilli, Pietro</creatorcontrib><title>A Functional Link between Dynamin and the Actin Cytoskeleton at Podosomes</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Cell transformation by Rous sarcoma virus results in a dramatic change of adhesion structures with the substratum. Adhesion plaques are replaced by dot-like attachment sites called podosomes. Podosomes are also found constitutively in motile nontransformed cells such as leukocytes, macrophages, and osteoclasts. They are represented by columnar arrays of actin which are perpendicular to the substratum and contain tubular invaginations of the plasma membrane. Given the similarity of these tubules to those generated by dynamin around a variety of membrane templates, we investigated whether dynamin is present at podosomes. Immunoreactivities for dynamin 2 and for the dynamin 2-binding protein endophilin 2 (SH3P8) were detected at podosomes of transformed cells and osteoclasts. Furthermore, GFP wild-type dynamin 2aa was targeted to podosomes. As shown by fluorescence recovery after photobleaching, GFP-dynamin 2aa and GFP-actin had a very rapid and similar turnover at podosomes. Expression of the GFP-dynamin 2 aaG273 Dabolished podosomes while GPF-dynaminK44 Awas targeted to podosomes but delayed actin turnover. These data demonstrate a functional link between a member of the dynamin family and actin at attachment sites between cells and the substratum.</description><subject>Actins</subject><subject>Actins - metabolism</subject><subject>Antibodies</subject><subject>Body Temperature - physiology</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Line, Transformed - drug effects</subject><subject>Cell Line, Transformed - metabolism</subject><subject>Cell Line, Transformed - ultrastructure</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell membranes</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Cellular immunity</subject><subject>Cyclosporine - pharmacology</subject><subject>Cyclosporins</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton - metabolism</subject><subject>Cytoskeleton - ultrastructure</subject><subject>Dynamin I</subject><subject>Dynamins</subject><subject>Endocytosis</subject><subject>Fluorescence</subject><subject>GTP Phosphohydrolases - metabolism</subject><subject>Microtubules - drug effects</subject><subject>Microtubules - metabolism</subject><subject>Microtubules - ultrastructure</subject><subject>Mutation - physiology</subject><subject>Neurons</subject><subject>Original</subject><subject>Osteoclasts</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoclasts - metabolism</subject><subject>Osteoclasts - ultrastructure</subject><subject>Viruses</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtP3DAUha2qqExpl-yqymLBLlM_Y3tTaTRAQRqJLtq15Tg3JUNi09ihmn-P0aDyWN3F-XR0dD-EjilZUqL5t61vllSSJVtypd6hBZWCVJoK8h4tCGG0MpLJQ_QxpS0hRCjBP6BDSgzRUpkFulrhizn43MfgBrzpwy1uIP8DCPhsF9zYB-xCi_MN4FWhAl7vcky3MECOJcr4Z2xjiiOkT-igc0OCz0_3CP2-OP-1vqw21z-u1qtN5YVRuQLRSk65ZN55X3tmOtc6TRtTt1JqU4NTAjxVTjgtvGRadG2tFCcCukZ7w4_Q933v3dyM0HoIeXKDvZv60U07G11vXyehv7F_4r1lVJd_PBacPhVM8e8MKduxTx6GwQWIc7KKMlkLIwt48gbcxnkqf0qlSxHFjagLVO0hP8WUJuj-L6HEPhqyxZAthiyzxVDhv76c_4LeKynAlz2wTTlOz3lNjRGSPwDo7ZZD</recordid><startdate>20000724</startdate><enddate>20000724</enddate><creator>Ochoa, Gian-Carlo</creator><creator>Slepnev, Vladimir I.</creator><creator>Neff, Lynn</creator><creator>Ringstad, Niels</creator><creator>Takei, Kohji</creator><creator>Daniell, Laurie</creator><creator>Kim, Warren</creator><creator>Cao, Hong</creator><creator>McNiven, Mark</creator><creator>Baron, Roland</creator><creator>de Camilli, Pietro</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20000724</creationdate><title>A Functional Link between Dynamin and the Actin Cytoskeleton at Podosomes</title><author>Ochoa, Gian-Carlo ; 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Adhesion plaques are replaced by dot-like attachment sites called podosomes. Podosomes are also found constitutively in motile nontransformed cells such as leukocytes, macrophages, and osteoclasts. They are represented by columnar arrays of actin which are perpendicular to the substratum and contain tubular invaginations of the plasma membrane. Given the similarity of these tubules to those generated by dynamin around a variety of membrane templates, we investigated whether dynamin is present at podosomes. Immunoreactivities for dynamin 2 and for the dynamin 2-binding protein endophilin 2 (SH3P8) were detected at podosomes of transformed cells and osteoclasts. Furthermore, GFP wild-type dynamin 2aa was targeted to podosomes. As shown by fluorescence recovery after photobleaching, GFP-dynamin 2aa and GFP-actin had a very rapid and similar turnover at podosomes. Expression of the GFP-dynamin 2 aaG273 Dabolished podosomes while GPF-dynaminK44 Awas targeted to podosomes but delayed actin turnover. These data demonstrate a functional link between a member of the dynamin family and actin at attachment sites between cells and the substratum.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>10908579</pmid><doi>10.1083/jcb.150.2.377</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Actins Actins - metabolism Antibodies Body Temperature - physiology Cell Adhesion - drug effects Cell Adhesion - physiology Cell Line, Transformed - drug effects Cell Line, Transformed - metabolism Cell Line, Transformed - ultrastructure Cell Membrane - drug effects Cell Membrane - metabolism Cell Membrane - ultrastructure Cell membranes Cells Cellular biology Cellular immunity Cyclosporine - pharmacology Cyclosporins Cytoskeleton - drug effects Cytoskeleton - metabolism Cytoskeleton - ultrastructure Dynamin I Dynamins Endocytosis Fluorescence GTP Phosphohydrolases - metabolism Microtubules - drug effects Microtubules - metabolism Microtubules - ultrastructure Mutation - physiology Neurons Original Osteoclasts Osteoclasts - drug effects Osteoclasts - metabolism Osteoclasts - ultrastructure Viruses |
| title | A Functional Link between Dynamin and the Actin Cytoskeleton at Podosomes |
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