A microfabricated scaffold for retinal progenitor cell grafting
Abstract Diseases that cause photoreceptor cell degeneration afflict millions of people, yet no restorative treatment exists for these blinding disorders. Replacement of photoreceptors using retinal progenitor cells (RPCs) represents a promising therapy for the treatment of retinal degeneration. Pre...
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| Veröffentlicht in: | Biomaterials 2008-02, Vol.29 (4), p.418-426 |
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| creator | Neeley, William L Redenti, Stephen Klassen, Henry Tao, Sarah Desai, Tejal Young, Michael J Langer, Robert |
| description | Abstract Diseases that cause photoreceptor cell degeneration afflict millions of people, yet no restorative treatment exists for these blinding disorders. Replacement of photoreceptors using retinal progenitor cells (RPCs) represents a promising therapy for the treatment of retinal degeneration. Previous studies have demonstrated the ability of polymer scaffolds to increase significantly both the survival and differentiation of RPCs. We report the microfabrication of a poly(glycerol-sebacate) scaffold with superior mechanical properties for the delivery of RPCs to the subretinal space. Using a replica molding technique, a porous poly(glycerol-sebacate) scaffold with a thickness of 45 μm was fabricated. Evaluation of the mechanical properties of this scaffold showed that the Young's modulus is about 5-fold lower and the maximum elongation at failure is about 10-fold higher than the previously reported RPC scaffolds. RPCs strongly adhered to the poly(glycerol-sebacate) scaffold, and endogenous fluorescence nearly doubled over a 2-day period before leveling off after 3 days. Immunohistochemistry revealed that cells grown on the scaffold for 7 days expressed a mixture of immature and mature markers, suggesting a tendency towards differentiation. We conclude that microfabricated poly(glycerol-sebacate) exhibits a number of novel properties for use as a scaffold for RPC delivery. |
| doi_str_mv | 10.1016/j.biomaterials.2007.10.007 |
| format | Article |
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Replacement of photoreceptors using retinal progenitor cells (RPCs) represents a promising therapy for the treatment of retinal degeneration. Previous studies have demonstrated the ability of polymer scaffolds to increase significantly both the survival and differentiation of RPCs. We report the microfabrication of a poly(glycerol-sebacate) scaffold with superior mechanical properties for the delivery of RPCs to the subretinal space. Using a replica molding technique, a porous poly(glycerol-sebacate) scaffold with a thickness of 45 μm was fabricated. Evaluation of the mechanical properties of this scaffold showed that the Young's modulus is about 5-fold lower and the maximum elongation at failure is about 10-fold higher than the previously reported RPC scaffolds. RPCs strongly adhered to the poly(glycerol-sebacate) scaffold, and endogenous fluorescence nearly doubled over a 2-day period before leveling off after 3 days. Immunohistochemistry revealed that cells grown on the scaffold for 7 days expressed a mixture of immature and mature markers, suggesting a tendency towards differentiation. We conclude that microfabricated poly(glycerol-sebacate) exhibits a number of novel properties for use as a scaffold for RPC delivery.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2007.10.007</identifier><identifier>PMID: 17961646</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Animals ; Biocompatibility ; Biomarkers ; Cell Adhesion ; Cell Culture Techniques - methods ; Cells, Cultured ; Decanoates - chemistry ; Dentistry ; Elastomer ; Glycerol - analogs & derivatives ; Glycerol - chemistry ; Immunohistochemistry ; Mice ; Microscopy, Electron, Scanning ; Molecular Structure ; Phenotype ; Polymers - chemistry ; Progenitor cell ; Retina ; Retina - cytology ; Retina - metabolism ; Scaffold ; Stem Cells - cytology ; Stem Cells - metabolism</subject><ispartof>Biomaterials, 2008-02, Vol.29 (4), p.418-426</ispartof><rights>Elsevier Ltd</rights><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c602t-c7751b3244e9ed0602f2f8c4af6057b1b8ddd45605bed9b11a5ee535bb5c631f3</citedby><cites>FETCH-LOGICAL-c602t-c7751b3244e9ed0602f2f8c4af6057b1b8ddd45605bed9b11a5ee535bb5c631f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961207007958$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17961646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neeley, William L</creatorcontrib><creatorcontrib>Redenti, Stephen</creatorcontrib><creatorcontrib>Klassen, Henry</creatorcontrib><creatorcontrib>Tao, Sarah</creatorcontrib><creatorcontrib>Desai, Tejal</creatorcontrib><creatorcontrib>Young, Michael J</creatorcontrib><creatorcontrib>Langer, Robert</creatorcontrib><title>A microfabricated scaffold for retinal progenitor cell grafting</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Diseases that cause photoreceptor cell degeneration afflict millions of people, yet no restorative treatment exists for these blinding disorders. Replacement of photoreceptors using retinal progenitor cells (RPCs) represents a promising therapy for the treatment of retinal degeneration. Previous studies have demonstrated the ability of polymer scaffolds to increase significantly both the survival and differentiation of RPCs. We report the microfabrication of a poly(glycerol-sebacate) scaffold with superior mechanical properties for the delivery of RPCs to the subretinal space. Using a replica molding technique, a porous poly(glycerol-sebacate) scaffold with a thickness of 45 μm was fabricated. Evaluation of the mechanical properties of this scaffold showed that the Young's modulus is about 5-fold lower and the maximum elongation at failure is about 10-fold higher than the previously reported RPC scaffolds. RPCs strongly adhered to the poly(glycerol-sebacate) scaffold, and endogenous fluorescence nearly doubled over a 2-day period before leveling off after 3 days. Immunohistochemistry revealed that cells grown on the scaffold for 7 days expressed a mixture of immature and mature markers, suggesting a tendency towards differentiation. We conclude that microfabricated poly(glycerol-sebacate) exhibits a number of novel properties for use as a scaffold for RPC delivery.</description><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biomarkers</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques - methods</subject><subject>Cells, Cultured</subject><subject>Decanoates - chemistry</subject><subject>Dentistry</subject><subject>Elastomer</subject><subject>Glycerol - analogs & derivatives</subject><subject>Glycerol - chemistry</subject><subject>Immunohistochemistry</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molecular Structure</subject><subject>Phenotype</subject><subject>Polymers - chemistry</subject><subject>Progenitor cell</subject><subject>Retina</subject><subject>Retina - cytology</subject><subject>Retina - metabolism</subject><subject>Scaffold</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUstuFDEQtBCILIFfQCMO3GZxe_yY4RAUhacUiQNwtvxoL15mx4s9Gyl_j0e7gsAlnFrdVV3udjUhL4CugYJ8tV3bmHZmxhzNWNaMUlWBdQ0PyAp61bdioOIhWVHgrB0ksDPypJQtrTnl7DE5A1WrkssVeXPZ7KLLKRibo6uavinOhJBG34SUm4xznMzY7HPa4BTnWnI4js0mm1CRzVPyKNQh8NkpnpNv7999vfrYXn_-8Onq8rp1krK5dUoJsB3jHAf0tNYCC73jJkgqlAXbe--5qIlFP1gAIxBFJ6wVTnYQunNycdTdH-wOvcNpzmbU-xx3Jt_qZKL-G5nid71JN5qB4t0gq8DLk0BOPw9YZr2LZVnFTJgORSsKwPqe30vsgEnBFb2XyKjkbBgWxddHYv3nUjKG32MD1YujeqvvOqoXRxeshtr8_O7if1pPFlbC2yMB6_ffRMy6uIiTQx8zuln7FP_vnYt_ZNwYp3oS4w-8xbJNhzwtPaAL01R_WW5rOS2qavcg-u4X4FvPKg</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Neeley, William L</creator><creator>Redenti, Stephen</creator><creator>Klassen, Henry</creator><creator>Tao, Sarah</creator><creator>Desai, Tejal</creator><creator>Young, Michael J</creator><creator>Langer, Robert</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080201</creationdate><title>A microfabricated scaffold for retinal progenitor cell grafting</title><author>Neeley, William L ; Redenti, Stephen ; Klassen, Henry ; Tao, Sarah ; Desai, Tejal ; Young, Michael J ; Langer, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c602t-c7751b3244e9ed0602f2f8c4af6057b1b8ddd45605bed9b11a5ee535bb5c631f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biomarkers</topic><topic>Cell Adhesion</topic><topic>Cell Culture Techniques - methods</topic><topic>Cells, Cultured</topic><topic>Decanoates - chemistry</topic><topic>Dentistry</topic><topic>Elastomer</topic><topic>Glycerol - analogs & derivatives</topic><topic>Glycerol - chemistry</topic><topic>Immunohistochemistry</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molecular Structure</topic><topic>Phenotype</topic><topic>Polymers - chemistry</topic><topic>Progenitor cell</topic><topic>Retina</topic><topic>Retina - cytology</topic><topic>Retina - metabolism</topic><topic>Scaffold</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neeley, William L</creatorcontrib><creatorcontrib>Redenti, Stephen</creatorcontrib><creatorcontrib>Klassen, Henry</creatorcontrib><creatorcontrib>Tao, Sarah</creatorcontrib><creatorcontrib>Desai, Tejal</creatorcontrib><creatorcontrib>Young, Michael J</creatorcontrib><creatorcontrib>Langer, Robert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neeley, William L</au><au>Redenti, Stephen</au><au>Klassen, Henry</au><au>Tao, Sarah</au><au>Desai, Tejal</au><au>Young, Michael J</au><au>Langer, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A microfabricated scaffold for retinal progenitor cell grafting</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>29</volume><issue>4</issue><spage>418</spage><epage>426</epage><pages>418-426</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Diseases that cause photoreceptor cell degeneration afflict millions of people, yet no restorative treatment exists for these blinding disorders. Replacement of photoreceptors using retinal progenitor cells (RPCs) represents a promising therapy for the treatment of retinal degeneration. Previous studies have demonstrated the ability of polymer scaffolds to increase significantly both the survival and differentiation of RPCs. We report the microfabrication of a poly(glycerol-sebacate) scaffold with superior mechanical properties for the delivery of RPCs to the subretinal space. Using a replica molding technique, a porous poly(glycerol-sebacate) scaffold with a thickness of 45 μm was fabricated. Evaluation of the mechanical properties of this scaffold showed that the Young's modulus is about 5-fold lower and the maximum elongation at failure is about 10-fold higher than the previously reported RPC scaffolds. RPCs strongly adhered to the poly(glycerol-sebacate) scaffold, and endogenous fluorescence nearly doubled over a 2-day period before leveling off after 3 days. Immunohistochemistry revealed that cells grown on the scaffold for 7 days expressed a mixture of immature and mature markers, suggesting a tendency towards differentiation. We conclude that microfabricated poly(glycerol-sebacate) exhibits a number of novel properties for use as a scaffold for RPC delivery.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>17961646</pmid><doi>10.1016/j.biomaterials.2007.10.007</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Advanced Basic Science Animals Biocompatibility Biomarkers Cell Adhesion Cell Culture Techniques - methods Cells, Cultured Decanoates - chemistry Dentistry Elastomer Glycerol - analogs & derivatives Glycerol - chemistry Immunohistochemistry Mice Microscopy, Electron, Scanning Molecular Structure Phenotype Polymers - chemistry Progenitor cell Retina Retina - cytology Retina - metabolism Scaffold Stem Cells - cytology Stem Cells - metabolism |
| title | A microfabricated scaffold for retinal progenitor cell grafting |
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