Gemin4: A Novel Component of the SMN Complex That Is Found in Both Gems and Nucleoli

The survival of motor neurons (SMN) protein, the product of the neurodegenerative disease spinal muscular atrophy (SMA) gene, is localized both in the cytoplasm and in discrete nuclear bodies called gems. In both compartments SMN is part of a large complex that contains several proteins including Ge...

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Veröffentlicht in:The Journal of cell biology 2000-03, Vol.148 (6), p.1177-1186
Hauptverfasser: Charroux, Bernard, Pellizzoni, Livio, Perkinson, Robert A., Yong, Jeongsik, Shevchenko, Andrej, Mann, Matthias, Dreyfuss, Gideon
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container_end_page 1186
container_issue 6
container_start_page 1177
container_title The Journal of cell biology
container_volume 148
creator Charroux, Bernard
Pellizzoni, Livio
Perkinson, Robert A.
Yong, Jeongsik
Shevchenko, Andrej
Mann, Matthias
Dreyfuss, Gideon
description The survival of motor neurons (SMN) protein, the product of the neurodegenerative disease spinal muscular atrophy (SMA) gene, is localized both in the cytoplasm and in discrete nuclear bodies called gems. In both compartments SMN is part of a large complex that contains several proteins including Gemin2 (formerly SIP1) and the DEAD box protein Gemin3. In the cytoplasm, the SMN complex is associated with snRNP Sm core proteins and plays a critical role in spliceosomal snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing by serving in the regeneration of spliceosomes. These functions are likely impaired in cells of SMA patients because they have reduced levels of functional SMN. Here, we report the identification by nanoelectrospray mass spectrometry of a novel component of the SMN complex that we name Gemin4. Gemin4 is associated in vivo with the SMN complex through a direct interaction with Gemin3. The tight interaction of Gemin4 with Gemin3 suggests that it could serve as a cofactor of this DEAD box protein. Gemin4 also interacts directly with several of the Sm core proteins. Monoclonal antibodies against Gemin4 efficiently immunoprecipitate the spliceosomal U snRNAs U1 and U5 from Xenopus oocytes cytoplasm. Immunolocalization experiments show that Gemin4 is colocalized with SMN in the cytoplasm and in gems. Interestingly, Gemin4 is also detected in the nucleoli, suggesting that the SMN complex may also function in preribosomal RNA processing or ribosome assembly.
doi_str_mv 10.1083/jcb.148.6.1177
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Monoclonal antibodies against Gemin4 efficiently immunoprecipitate the spliceosomal U snRNAs U1 and U5 from Xenopus oocytes cytoplasm. Immunolocalization experiments show that Gemin4 is colocalized with SMN in the cytoplasm and in gems. 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Monoclonal antibodies against Gemin4 efficiently immunoprecipitate the spliceosomal U snRNAs U1 and U5 from Xenopus oocytes cytoplasm. Immunolocalization experiments show that Gemin4 is colocalized with SMN in the cytoplasm and in gems. Interestingly, Gemin4 is also detected in the nucleoli, suggesting that the SMN complex may also function in preribosomal RNA processing or ribosome assembly.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>10725331</pmid><doi>10.1083/jcb.148.6.1177</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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ispartof The Journal of cell biology, 2000-03, Vol.148 (6), p.1177-1186
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection
subjects Animals
Antibodies
Antibodies, Monoclonal
Cell nucleolus
Cell Nucleolus - physiology
Cell Nucleolus - ultrastructure
Cell Nucleus - physiology
Cell Nucleus - ultrastructure
Cellular biology
Cyclic AMP Response Element-Binding Protein
Cytoplasm
Cytoplasm - physiology
Cytoplasm - ultrastructure
DEAD Box Protein 20
DEAD-box RNA Helicases
Female
HeLa Cells
Humans
Immunoprecipitation
Minor Histocompatibility Antigens
Models, Molecular
Muscular Atrophy, Spinal - genetics
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neurological disorders
Nuclear Proteins - analysis
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Oocytes
Oocytes - physiology
Oocytes - ultrastructure
Original
Proteins
Ribonucleic acid
Ribonucleoproteins, Small Nuclear - metabolism
RNA
RNA Helicases - analysis
RNA Helicases - metabolism
RNA-Binding Proteins
Small nuclear ribonucleoproteins
Small nuclear RNA
SMN Complex Proteins
Spinal muscular atrophy
Xenopus laevis
title Gemin4: A Novel Component of the SMN Complex That Is Found in Both Gems and Nucleoli
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