A Fluorescent Resonant Energy Transfer-Based Biosensor Reveals Transient and Regional Myosin Light Chain Kinase Activation in Lamella and Cleavage Furrows

Approaches with high spatial and temporal resolution are required to understand the regulation of nonmuscle myosin II in vivo. Using fluorescence resonance energy transfer we have produced a novel biosensor allowing simultaneous determination of myosin light chain kinase (MLCK) localization and its...

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Veröffentlicht in:	The Journal of cell biology 2002-02, Vol.156 (3), p.543-553
Hauptverfasser:	Chew, Teng-Leong, Wolf, Wendy A., Gallagher, Patricia J., Matsumura, Fumio, Chisholm, Rex L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biosensing Techniques - methods Calcium - metabolism Calcium Signaling - physiology Calmodulin - metabolism Cell Division - physiology Cell lines Cell motility Cell Movement - physiology Cell Polarity - physiology Cells COS cells Cytokinesis Cytoskeleton - metabolism Dipodomys Endothelial cells HeLa cells Intracellular Membranes - metabolism Microscopy, Fluorescence - methods Myosin-Light-Chain Kinase - metabolism Myosins - metabolism Phosphorylation Pseudopodia - metabolism Smooth muscle Stress fibers
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