Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg...
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| Veröffentlicht in: | The Journal of cell biology 2005-09, Vol.170 (7), p.1047-1055 |
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| container_title | The Journal of cell biology |
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| creator | Kinoshita, Kazuhisa Noetzel, Tim L Pelletier, Laurence Mechtler, Karl Drechsel, David N Schwager, Anne Lee, Mike Raff, Jordan W Hyman, Anthony A |
| description | Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis. |
| doi_str_mv | 10.1083/jcb.200503023 |
| format | Article |
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We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.200503023</identifier><identifier>PMID: 16172205</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Animals ; Antibodies ; Aurora Kinases ; Cell cycle ; Cell Cycle Proteins - physiology ; Cell Extracts ; Cells ; Cellular biology ; Centrosome - physiology ; Centrosomes ; Eggs ; Gene expression regulation ; Kinesin - metabolism ; Microtubule-Associated Proteins - metabolism ; Microtubule-Associated Proteins - physiology ; Microtubules ; Microtubules - chemistry ; Microtubules - metabolism ; Mitosis ; Mitotic spindle apparatus ; Oocytes - chemistry ; Phosphorylation ; Polymers ; Protein Kinases - physiology ; Protein-Serine-Threonine Kinases ; Proteins ; Transcription Factors - physiology ; Xenopus laevis ; Xenopus Proteins - metabolism ; Xenopus Proteins - physiology</subject><ispartof>The Journal of cell biology, 2005-09, Vol.170 (7), p.1047-1055</ispartof><rights>Copyright 2005 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Sep 26, 2005</rights><rights>Copyright © 2005, The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-c08ad5a9ad2338a865f0c66776c32d30747a623c900d0afc178eaa62358c5c9f3</citedby><cites>FETCH-LOGICAL-c554t-c08ad5a9ad2338a865f0c66776c32d30747a623c900d0afc178eaa62358c5c9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16172205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kinoshita, Kazuhisa</creatorcontrib><creatorcontrib>Noetzel, Tim L</creatorcontrib><creatorcontrib>Pelletier, Laurence</creatorcontrib><creatorcontrib>Mechtler, Karl</creatorcontrib><creatorcontrib>Drechsel, David N</creatorcontrib><creatorcontrib>Schwager, Anne</creatorcontrib><creatorcontrib>Lee, Mike</creatorcontrib><creatorcontrib>Raff, Jordan W</creatorcontrib><creatorcontrib>Hyman, Anthony A</creatorcontrib><title>Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. 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We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Aurora Kinases</subject><subject>Cell cycle</subject><subject>Cell Cycle Proteins - physiology</subject><subject>Cell Extracts</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Centrosome - physiology</subject><subject>Centrosomes</subject><subject>Eggs</subject><subject>Gene expression regulation</subject><subject>Kinesin - metabolism</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Microtubule-Associated Proteins - physiology</subject><subject>Microtubules</subject><subject>Microtubules - chemistry</subject><subject>Microtubules - metabolism</subject><subject>Mitosis</subject><subject>Mitotic spindle apparatus</subject><subject>Oocytes - chemistry</subject><subject>Phosphorylation</subject><subject>Polymers</subject><subject>Protein Kinases - physiology</subject><subject>Protein-Serine-Threonine Kinases</subject><subject>Proteins</subject><subject>Transcription Factors - physiology</subject><subject>Xenopus laevis</subject><subject>Xenopus Proteins - metabolism</subject><subject>Xenopus Proteins - physiology</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhS0EoqGwZIfAYsFu2mt7_JhNpSjiJVViQbu2bjye1mFmnNozSPn3OE0UHhsWlmXfz_cen0PIawYXDIy43Lj1BQeQIICLJ2TBZA2VYTU8JQsAzqpGcnlGXuS8AYBa1-I5OWOKac5BLsiwnFNMSJd0ex9zWWnX4xTiSGNHb5arlbgcMP8IIw2ZJv8wh-Rb2sVEnR-nFHMcfNX6rR_bcqZDcClO83ruPcWc_bDud7Q8HsIUc8gvybMO--xfHfdzcvvp483qS3X97fPX1fK6clLWU-XAYCuxwZYLYdAo2YFTSmvlBG8F6Fqj4sI1AC1g55g2Hvc30jjpmk6ck6tD3-28Hnz7KBV7u01hwLSzEYP9uzKGe3sXf1rOdDGwLg0-HBuk-DD7PNkhZOf7Hkcf52yVUdw0Gv4LctC8kcYU8P0_4CbOaSwuPA4teRhdoOoAFRdzTr47SWZg93HbErc9xV34t3_-8zd9zLcAbw7AJk8xnepCSSPYXtS7Q7nDaPEuhWxvv3NgAlgZ0DRc_AKrnbnC</recordid><startdate>20050926</startdate><enddate>20050926</enddate><creator>Kinoshita, Kazuhisa</creator><creator>Noetzel, Tim L</creator><creator>Pelletier, Laurence</creator><creator>Mechtler, Karl</creator><creator>Drechsel, David N</creator><creator>Schwager, Anne</creator><creator>Lee, Mike</creator><creator>Raff, Jordan W</creator><creator>Hyman, Anthony A</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050926</creationdate><title>Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis</title><author>Kinoshita, Kazuhisa ; 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| subjects | Animals Antibodies Aurora Kinases Cell cycle Cell Cycle Proteins - physiology Cell Extracts Cells Cellular biology Centrosome - physiology Centrosomes Eggs Gene expression regulation Kinesin - metabolism Microtubule-Associated Proteins - metabolism Microtubule-Associated Proteins - physiology Microtubules Microtubules - chemistry Microtubules - metabolism Mitosis Mitotic spindle apparatus Oocytes - chemistry Phosphorylation Polymers Protein Kinases - physiology Protein-Serine-Threonine Kinases Proteins Transcription Factors - physiology Xenopus laevis Xenopus Proteins - metabolism Xenopus Proteins - physiology |
| title | Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis |
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