Function and Regulation of Maskin, a TACC Family Protein, in Microtubule Growth during Mitosis
The Xenopus protein Maskin has been previously identified and characterized in the context of its role in translational control during oocyte maturation. Maskin belongs to the TACC protein family. In other systems, members of this family have been shown to localize to centrosomes during mitosis and...
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| Veröffentlicht in: | The Journal of cell biology 2005-09, Vol.170 (7), p.1057-1066 |
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| container_title | The Journal of cell biology |
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| creator | Peset, Isabel Seiler, Jeanette Sardon, Teresa Bejarano, Luis A. Rybina, Sonja Vernos, Isabelle |
| description | The Xenopus protein Maskin has been previously identified and characterized in the context of its role in translational control during oocyte maturation. Maskin belongs to the TACC protein family. In other systems, members of this family have been shown to localize to centrosomes during mitosis and play a role in microtubule stabilization. Here we have examined the putative role of Maskin in spindle assembly and centrosome aster formation in the Xenopus egg extract system. Depletion and reconstitution experiments indicate that Maskin plays an essential role for microtubule assembly during M-phase. We show that Maskin interacts with XMAP215 and Eg2, the Xenopus Aurora A kinase in vitro and in the egg extract. We propose that Maskin and XMAP215 cooperate to oppose the destabilizing activity of XKCM1 therefore promoting microtubule growth from the centrosome and contributing to the determination of microtubule steady-state length. Further more, we show that Maskin localization and function is regulated by Eg2 phosphorylation. |
| doi_str_mv | 10.1083/jcb.200504037 |
| format | Article |
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Maskin belongs to the TACC protein family. In other systems, members of this family have been shown to localize to centrosomes during mitosis and play a role in microtubule stabilization. Here we have examined the putative role of Maskin in spindle assembly and centrosome aster formation in the Xenopus egg extract system. Depletion and reconstitution experiments indicate that Maskin plays an essential role for microtubule assembly during M-phase. We show that Maskin interacts with XMAP215 and Eg2, the Xenopus Aurora A kinase in vitro and in the egg extract. We propose that Maskin and XMAP215 cooperate to oppose the destabilizing activity of XKCM1 therefore promoting microtubule growth from the centrosome and contributing to the determination of microtubule steady-state length. Further more, we show that Maskin localization and function is regulated by Eg2 phosphorylation.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.200504037</identifier><identifier>PMID: 16172207</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Animals ; Antibodies ; Aurora Kinases ; Cell cycle ; Cell Cycle Proteins - metabolism ; Cell Extracts - chemistry ; Cell Line ; Cells ; Cellular biology ; Centrosome - chemistry ; Centrosome - physiology ; Centrosomes ; Egg Proteins - physiology ; Eggs ; Kinesin - metabolism ; Microtubule-Associated Proteins - chemistry ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - metabolism ; Microtubule-Associated Proteins - physiology ; Microtubules ; Microtubules - chemistry ; Microtubules - metabolism ; Mitosis ; Mitosis - physiology ; Mitotic spindle apparatus ; Ova ; Phosphorylation ; Physiological regulation ; Protein Kinases - metabolism ; Protein-Serine-Threonine Kinases ; Proteins ; Recombinant Proteins - analysis ; Recombinant Proteins - genetics ; Spindle Apparatus - chemistry ; Spindle Apparatus - metabolism ; Transcription Factors - chemistry ; Transcription Factors - genetics ; Transcription Factors - physiology ; Xenopus ; Xenopus Proteins - chemistry ; Xenopus Proteins - genetics ; Xenopus Proteins - metabolism ; Xenopus Proteins - physiology</subject><ispartof>The Journal of cell biology, 2005-09, Vol.170 (7), p.1057-1066</ispartof><rights>Copyright 2005 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Sep 26, 2005</rights><rights>Copyright © 2005, The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-914b4320abc3d506ac72c0dd1d42a3cfbc3e681513878af28ca272c424443e7e3</citedby><cites>FETCH-LOGICAL-c530t-914b4320abc3d506ac72c0dd1d42a3cfbc3e681513878af28ca272c424443e7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16172207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peset, Isabel</creatorcontrib><creatorcontrib>Seiler, Jeanette</creatorcontrib><creatorcontrib>Sardon, Teresa</creatorcontrib><creatorcontrib>Bejarano, Luis A.</creatorcontrib><creatorcontrib>Rybina, Sonja</creatorcontrib><creatorcontrib>Vernos, Isabelle</creatorcontrib><title>Function and Regulation of Maskin, a TACC Family Protein, in Microtubule Growth during Mitosis</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>The Xenopus protein Maskin has been previously identified and characterized in the context of its role in translational control during oocyte maturation. Maskin belongs to the TACC protein family. In other systems, members of this family have been shown to localize to centrosomes during mitosis and play a role in microtubule stabilization. Here we have examined the putative role of Maskin in spindle assembly and centrosome aster formation in the Xenopus egg extract system. Depletion and reconstitution experiments indicate that Maskin plays an essential role for microtubule assembly during M-phase. We show that Maskin interacts with XMAP215 and Eg2, the Xenopus Aurora A kinase in vitro and in the egg extract. We propose that Maskin and XMAP215 cooperate to oppose the destabilizing activity of XKCM1 therefore promoting microtubule growth from the centrosome and contributing to the determination of microtubule steady-state length. Further more, we show that Maskin localization and function is regulated by Eg2 phosphorylation.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Aurora Kinases</subject><subject>Cell cycle</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell Extracts - chemistry</subject><subject>Cell Line</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Centrosome - chemistry</subject><subject>Centrosome - physiology</subject><subject>Centrosomes</subject><subject>Egg Proteins - physiology</subject><subject>Eggs</subject><subject>Kinesin - metabolism</subject><subject>Microtubule-Associated Proteins - chemistry</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Microtubule-Associated Proteins - physiology</subject><subject>Microtubules</subject><subject>Microtubules - chemistry</subject><subject>Microtubules - metabolism</subject><subject>Mitosis</subject><subject>Mitosis - physiology</subject><subject>Mitotic spindle apparatus</subject><subject>Ova</subject><subject>Phosphorylation</subject><subject>Physiological regulation</subject><subject>Protein Kinases - metabolism</subject><subject>Protein-Serine-Threonine Kinases</subject><subject>Proteins</subject><subject>Recombinant Proteins - analysis</subject><subject>Recombinant Proteins - genetics</subject><subject>Spindle Apparatus - chemistry</subject><subject>Spindle Apparatus - metabolism</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - physiology</subject><subject>Xenopus</subject><subject>Xenopus Proteins - chemistry</subject><subject>Xenopus Proteins - genetics</subject><subject>Xenopus Proteins - metabolism</subject><subject>Xenopus Proteins - physiology</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhS1ERUNhyQ4hi0VXTLl-jZ0NUhU1pVIrECpbLI_HkzpM7NYeg_rvcZsoPDasrHvPp2P7HIReETghoNj7te1OKIAADkw-QTMiODSKcHiKZgCUNHNBxSF6nvMaALjk7Bk6JC2RlIKcoW_LEuzkY8Am9PiLW5XRPI5xwFcmf_fhHTb4-nSxwEuz8eM9_pzi5B7WPuArb-tUujI6fJ7iz-kG9yX5sKrKFLPPL9DBYMbsXu7OI_R1eXa9-Nhcfjq_WJxeNlYwmJo54R1nFExnWS-gNVZSC31Pek4Ns0Ndu1YRQZiSygxUWUMrwSnnnDnp2BH6sPW9Ld3G9daFKZlR3ya_MeleR-P130rwN3oVf2hKJKkBVYPjnUGKd8XlSW98tm4cTXCxZN2qlirJ_w_WVBmAlBV8-w-4jiWFmsLjpUClmFeo2UI1x5yTG_ZPJqAf-tW1X73vt_Jv_vznb3pXaAVeb4F1nmLa66wVipE5-wUTuamJ</recordid><startdate>20050926</startdate><enddate>20050926</enddate><creator>Peset, Isabel</creator><creator>Seiler, Jeanette</creator><creator>Sardon, Teresa</creator><creator>Bejarano, Luis A.</creator><creator>Rybina, Sonja</creator><creator>Vernos, Isabelle</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050926</creationdate><title>Function and Regulation of Maskin, a TACC Family Protein, in Microtubule Growth during Mitosis</title><author>Peset, Isabel ; Seiler, Jeanette ; Sardon, Teresa ; Bejarano, Luis A. ; Rybina, Sonja ; Vernos, Isabelle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-914b4320abc3d506ac72c0dd1d42a3cfbc3e681513878af28ca272c424443e7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Aurora Kinases</topic><topic>Cell cycle</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell Extracts - chemistry</topic><topic>Cell Line</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Centrosome - chemistry</topic><topic>Centrosome - physiology</topic><topic>Centrosomes</topic><topic>Egg Proteins - physiology</topic><topic>Eggs</topic><topic>Kinesin - metabolism</topic><topic>Microtubule-Associated Proteins - chemistry</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>Microtubule-Associated Proteins - physiology</topic><topic>Microtubules</topic><topic>Microtubules - chemistry</topic><topic>Microtubules - metabolism</topic><topic>Mitosis</topic><topic>Mitosis - physiology</topic><topic>Mitotic spindle apparatus</topic><topic>Ova</topic><topic>Phosphorylation</topic><topic>Physiological regulation</topic><topic>Protein Kinases - metabolism</topic><topic>Protein-Serine-Threonine Kinases</topic><topic>Proteins</topic><topic>Recombinant Proteins - analysis</topic><topic>Recombinant Proteins - genetics</topic><topic>Spindle Apparatus - chemistry</topic><topic>Spindle Apparatus - metabolism</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - physiology</topic><topic>Xenopus</topic><topic>Xenopus Proteins - chemistry</topic><topic>Xenopus Proteins - genetics</topic><topic>Xenopus Proteins - metabolism</topic><topic>Xenopus Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peset, Isabel</creatorcontrib><creatorcontrib>Seiler, Jeanette</creatorcontrib><creatorcontrib>Sardon, Teresa</creatorcontrib><creatorcontrib>Bejarano, Luis A.</creatorcontrib><creatorcontrib>Rybina, Sonja</creatorcontrib><creatorcontrib>Vernos, Isabelle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peset, Isabel</au><au>Seiler, Jeanette</au><au>Sardon, Teresa</au><au>Bejarano, Luis A.</au><au>Rybina, Sonja</au><au>Vernos, Isabelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Function and Regulation of Maskin, a TACC Family Protein, in Microtubule Growth during Mitosis</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>2005-09-26</date><risdate>2005</risdate><volume>170</volume><issue>7</issue><spage>1057</spage><epage>1066</epage><pages>1057-1066</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>The Xenopus protein Maskin has been previously identified and characterized in the context of its role in translational control during oocyte maturation. 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| subjects | Animals Antibodies Aurora Kinases Cell cycle Cell Cycle Proteins - metabolism Cell Extracts - chemistry Cell Line Cells Cellular biology Centrosome - chemistry Centrosome - physiology Centrosomes Egg Proteins - physiology Eggs Kinesin - metabolism Microtubule-Associated Proteins - chemistry Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism Microtubule-Associated Proteins - physiology Microtubules Microtubules - chemistry Microtubules - metabolism Mitosis Mitosis - physiology Mitotic spindle apparatus Ova Phosphorylation Physiological regulation Protein Kinases - metabolism Protein-Serine-Threonine Kinases Proteins Recombinant Proteins - analysis Recombinant Proteins - genetics Spindle Apparatus - chemistry Spindle Apparatus - metabolism Transcription Factors - chemistry Transcription Factors - genetics Transcription Factors - physiology Xenopus Xenopus Proteins - chemistry Xenopus Proteins - genetics Xenopus Proteins - metabolism Xenopus Proteins - physiology |
| title | Function and Regulation of Maskin, a TACC Family Protein, in Microtubule Growth during Mitosis |
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