Inhibition of cell movement and proliferation by cell-cell contact-induced interaction of Necl-5 with nectin-3
Immunoglobulin-like Necl-5/Tage4/poliovirus receptor (PVR)/CD155, originally identified as the PVR, has been shown to be up-regulated in cancer cells and to enhance growth factor-induced cell movement and proliferation. In addition, Necl-5 heterophilically trans-interacts with nectin-3, a cell-cell...
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| Veröffentlicht in: | The Journal of cell biology 2005-10, Vol.171 (1), p.165-173 |
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| container_title | The Journal of cell biology |
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| creator | Fujito, Tsutomu Ikeda, Wataru Kakunaga, Shigeki Minami, Yukiko Kajita, Mihoko Sakamoto, Yasuhisa Monden, Morito Takai, Yoshimi |
| description | Immunoglobulin-like Necl-5/Tage4/poliovirus receptor (PVR)/CD155, originally identified as the PVR, has been shown to be up-regulated in cancer cells and to enhance growth factor-induced cell movement and proliferation. In addition, Necl-5 heterophilically trans-interacts with nectin-3, a cell-cell adhesion molecule known to form adherens junctions in cooperation with cadherin. We show here that Necl-5 was down-regulated from cell surface upon cell-cell contacts in NIH3T3 cells. This down-regulation of Necl-5 was initiated by its interaction with nectin-3 and was mainly mediated by clathrin-dependent endocytosis. Then, the down-regulation of Necl-5 induced in this way reduced movement and proliferation of NIH3T3 cells. These results indicate that the down-regulation of Necl-5 induced by its interaction with nectin-3 upon cell-cell contacts may be at least one mechanism underlying contact inhibition of cell movement and proliferation. |
| doi_str_mv | 10.1083/jcb.200501090 |
| format | Article |
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In addition, Necl-5 heterophilically trans-interacts with nectin-3, a cell-cell adhesion molecule known to form adherens junctions in cooperation with cadherin. We show here that Necl-5 was down-regulated from cell surface upon cell-cell contacts in NIH3T3 cells. This down-regulation of Necl-5 was initiated by its interaction with nectin-3 and was mainly mediated by clathrin-dependent endocytosis. Then, the down-regulation of Necl-5 induced in this way reduced movement and proliferation of NIH3T3 cells. 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| subjects | Animals Antigens, Neoplasm - genetics Antigens, Neoplasm - metabolism Blood cells Cell adhesion & migration Cell Adhesion - drug effects Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism Cell culture techniques Cell lines Cell motility Cell Movement - drug effects Cell Proliferation - drug effects Cellular biology Clathrin - metabolism Cultured cells Down regulation Endocytosis Mice Natural killer cells Nectins Neoplasm Proteins - genetics Neoplasm Proteins - metabolism NIH 3T3 Cells Proteins RNA, Small Interfering - genetics Small interfering RNA |
| title | Inhibition of cell movement and proliferation by cell-cell contact-induced interaction of Necl-5 with nectin-3 |
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