A novel chromatography system to isolate active ribosomes from pathogenic bacteria
We have developed a novel chromatography for the rapid isolation of active ribosomes from bacteria without the use of harsh conditions or lengthy procedures that damage ribosomes. Ribosomes interact with an alkyl linker attached to the resin, apparently through their RNA component. Examples are give...
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| Veröffentlicht in: | RNA (Cambridge) 2008-01, Vol.14 (1), p.188-195 |
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| creator | Maguire, Bruce A Wondrack, Lillian M Contillo, Leonard G Xu, Zuoyu |
| description | We have developed a novel chromatography for the rapid isolation of active ribosomes from bacteria without the use of harsh conditions or lengthy procedures that damage ribosomes. Ribosomes interact with an alkyl linker attached to the resin, apparently through their RNA component. Examples are given with ribosomes from Escherichia coli, Deinococcus radiodurans, and with clinical isolates of Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA). The ribosomes obtained by this method are unusually intact, so that highly active ribosomes can now be isolated from the clinical isolates, enabling significantly improved in vitro functional assays that will greatly assist the discovery and development of new ribosomally targeted antibiotics. |
| doi_str_mv | 10.1261/rna.692408 |
| format | Article |
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Ribosomes interact with an alkyl linker attached to the resin, apparently through their RNA component. Examples are given with ribosomes from Escherichia coli, Deinococcus radiodurans, and with clinical isolates of Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA). The ribosomes obtained by this method are unusually intact, so that highly active ribosomes can now be isolated from the clinical isolates, enabling significantly improved in vitro functional assays that will greatly assist the discovery and development of new ribosomally targeted antibiotics.</description><identifier>ISSN: 1355-8382</identifier><identifier>EISSN: 1469-9001</identifier><identifier>DOI: 10.1261/rna.692408</identifier><identifier>PMID: 17998293</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Bacteria - pathogenicity ; Bacteria - ultrastructure ; Chromatography, Liquid - methods ; Mass Spectrometry ; Method ; Ribosomes ; RNA, Bacterial - chemistry ; RNA, Bacterial - isolation & purification</subject><ispartof>RNA (Cambridge), 2008-01, Vol.14 (1), p.188-195</ispartof><rights>Copyright © 2008 RNA Society 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-15fa55647dac71c493de2abd5310e28727c4beca51fd6e8d6b7667c66842d9153</citedby><cites>FETCH-LOGICAL-c442t-15fa55647dac71c493de2abd5310e28727c4beca51fd6e8d6b7667c66842d9153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151033/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151033/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17998293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maguire, Bruce A</creatorcontrib><creatorcontrib>Wondrack, Lillian M</creatorcontrib><creatorcontrib>Contillo, Leonard G</creatorcontrib><creatorcontrib>Xu, Zuoyu</creatorcontrib><title>A novel chromatography system to isolate active ribosomes from pathogenic bacteria</title><title>RNA (Cambridge)</title><addtitle>RNA</addtitle><description>We have developed a novel chromatography for the rapid isolation of active ribosomes from bacteria without the use of harsh conditions or lengthy procedures that damage ribosomes. 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The ribosomes obtained by this method are unusually intact, so that highly active ribosomes can now be isolated from the clinical isolates, enabling significantly improved in vitro functional assays that will greatly assist the discovery and development of new ribosomally targeted antibiotics.</description><subject>Bacteria - pathogenicity</subject><subject>Bacteria - ultrastructure</subject><subject>Chromatography, Liquid - methods</subject><subject>Mass Spectrometry</subject><subject>Method</subject><subject>Ribosomes</subject><subject>RNA, Bacterial - chemistry</subject><subject>RNA, Bacterial - isolation & purification</subject><issn>1355-8382</issn><issn>1469-9001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1LAzEQhoMotlYv_gDJyYOwdfO5uxehFL-gIIieQzY720Z2NzVJC_33Rlr8OM0w88w7w7wIXZJ8Sqgkt37QU1lRnpdHaEy4rLIqz8lxypkQWclKOkJnIXykIkvtUzQiRVWVtGJj9DrDg9tCh83Ku15Ht_R6vdrhsAsRehwdtsF1OgLWJtotYG9rF1wPAbdpAK91XLklDNbgOhHgrT5HJ63uAlwc4gS9P9y_zZ-yxcvj83y2yAznNGZEtFoIyYtGm4IYXrEGqK4bwUgOtCxoYXgNRgvSNhLKRtaFlIWRsuS0qYhgE3S3111v6h4aA0P0ulNrb3vtd8ppq_53BrtSS7dVlAiSM5YErg8C3n1uIETV22Cg6_QAbhNUkZN0VckTeLMHjXcheGh_lpBcfVugkgVqb0GCr_6e9Ysefs6-APnihFg</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Maguire, Bruce A</creator><creator>Wondrack, Lillian M</creator><creator>Contillo, Leonard G</creator><creator>Xu, Zuoyu</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080101</creationdate><title>A novel chromatography system to isolate active ribosomes from pathogenic bacteria</title><author>Maguire, Bruce A ; Wondrack, Lillian M ; Contillo, Leonard G ; Xu, Zuoyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-15fa55647dac71c493de2abd5310e28727c4beca51fd6e8d6b7667c66842d9153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bacteria - pathogenicity</topic><topic>Bacteria - ultrastructure</topic><topic>Chromatography, Liquid - methods</topic><topic>Mass Spectrometry</topic><topic>Method</topic><topic>Ribosomes</topic><topic>RNA, Bacterial - chemistry</topic><topic>RNA, Bacterial - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maguire, Bruce A</creatorcontrib><creatorcontrib>Wondrack, Lillian M</creatorcontrib><creatorcontrib>Contillo, Leonard G</creatorcontrib><creatorcontrib>Xu, Zuoyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RNA (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maguire, Bruce A</au><au>Wondrack, Lillian M</au><au>Contillo, Leonard G</au><au>Xu, Zuoyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel chromatography system to isolate active ribosomes from pathogenic bacteria</atitle><jtitle>RNA (Cambridge)</jtitle><addtitle>RNA</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>14</volume><issue>1</issue><spage>188</spage><epage>195</epage><pages>188-195</pages><issn>1355-8382</issn><eissn>1469-9001</eissn><abstract>We have developed a novel chromatography for the rapid isolation of active ribosomes from bacteria without the use of harsh conditions or lengthy procedures that damage ribosomes. Ribosomes interact with an alkyl linker attached to the resin, apparently through their RNA component. Examples are given with ribosomes from Escherichia coli, Deinococcus radiodurans, and with clinical isolates of Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA). The ribosomes obtained by this method are unusually intact, so that highly active ribosomes can now be isolated from the clinical isolates, enabling significantly improved in vitro functional assays that will greatly assist the discovery and development of new ribosomally targeted antibiotics.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>17998293</pmid><doi>10.1261/rna.692408</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | RNA (Cambridge), 2008-01, Vol.14 (1), p.188-195 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacteria - pathogenicity Bacteria - ultrastructure Chromatography, Liquid - methods Mass Spectrometry Method Ribosomes RNA, Bacterial - chemistry RNA, Bacterial - isolation & purification |
| title | A novel chromatography system to isolate active ribosomes from pathogenic bacteria |
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