Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian MSS4-Rab8 GTPase protein complex
Rab GTPases function as ubiquitous key regulators of membrane‐vesicle transport in eukaryotic cells. MSS4 is an evolutionarily conserved protein that binds to exocytotic Rabs and facilitates nucleotide release. The MSS4 protein in complex with nucleotide‐free Rab8 GTPase has been purified and crysta...
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| Veröffentlicht in: | Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2006-02, Vol.62 (2), p.113-116 |
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| container_title | Acta crystallographica. Section F, Structural biology and crystallization communications |
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| creator | Itzen, Aymelt Bleimling, Nathalie Ignatev, Alexander Pylypenko, Olena Rak, Alexey |
| description | Rab GTPases function as ubiquitous key regulators of membrane‐vesicle transport in eukaryotic cells. MSS4 is an evolutionarily conserved protein that binds to exocytotic Rabs and facilitates nucleotide release. The MSS4 protein in complex with nucleotide‐free Rab8 GTPase has been purified and crystallized in a form suitable for structure analysis. The crystals belonged to space group P1, with unit‐cell parameters a = 40.92, b = 49.85, c = 83.48 Å, α = 102.88, β = 97.46, γ = 90.12°. A complete data set has been collected to 2 Å resolution. |
| doi_str_mv | 10.1107/S1744309105042995 |
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MSS4 is an evolutionarily conserved protein that binds to exocytotic Rabs and facilitates nucleotide release. The MSS4 protein in complex with nucleotide‐free Rab8 GTPase has been purified and crystallized in a form suitable for structure analysis. The crystals belonged to space group P1, with unit‐cell parameters a = 40.92, b = 49.85, c = 83.48 Å, α = 102.88, β = 97.46, γ = 90.12°. A complete data set has been collected to 2 Å resolution.</description><identifier>ISSN: 1744-3091</identifier><identifier>EISSN: 1744-3091</identifier><identifier>DOI: 10.1107/S1744309105042995</identifier><identifier>PMID: 16511278</identifier><language>eng</language><publisher>5 Abbey Square, Chester, Cheshire CH1 2HU, England: Blackwell Publishing Ltd</publisher><subject>1,2,3‐heptanetriole ; 3-heptanetriole ; Animals ; CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY ; CRYSTALLIZATION ; Crystallization Communications ; Crystallography, X-Ray ; CRYSTALS ; GEF ; GTP Phosphohydrolases - chemistry ; GTP Phosphohydrolases - isolation & purification ; Guanine Nucleotide Exchange Factors - chemistry ; Guanine Nucleotide Exchange Factors - isolation & purification ; Humans ; Life Sciences ; MEMBRANES ; Mice ; nucleotide release ; PROTEINS ; rab GTP-Binding Proteins - chemistry ; rab GTP-Binding Proteins - isolation & purification ; RESOLUTION ; small Rab GTPases ; SPACE GROUPS ; vesicular membrane traffic</subject><ispartof>Acta crystallographica. 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Section F, Structural biology and crystallization communications</title><addtitle>Acta Cryst. F</addtitle><description>Rab GTPases function as ubiquitous key regulators of membrane‐vesicle transport in eukaryotic cells. MSS4 is an evolutionarily conserved protein that binds to exocytotic Rabs and facilitates nucleotide release. The MSS4 protein in complex with nucleotide‐free Rab8 GTPase has been purified and crystallized in a form suitable for structure analysis. The crystals belonged to space group P1, with unit‐cell parameters a = 40.92, b = 49.85, c = 83.48 Å, α = 102.88, β = 97.46, γ = 90.12°. A complete data set has been collected to 2 Å resolution.</description><subject>1,2,3‐heptanetriole</subject><subject>3-heptanetriole</subject><subject>Animals</subject><subject>CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY</subject><subject>CRYSTALLIZATION</subject><subject>Crystallization Communications</subject><subject>Crystallography, X-Ray</subject><subject>CRYSTALS</subject><subject>GEF</subject><subject>GTP Phosphohydrolases - chemistry</subject><subject>GTP Phosphohydrolases - isolation & purification</subject><subject>Guanine Nucleotide Exchange Factors - chemistry</subject><subject>Guanine Nucleotide Exchange Factors - isolation & purification</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>MEMBRANES</subject><subject>Mice</subject><subject>nucleotide release</subject><subject>PROTEINS</subject><subject>rab GTP-Binding Proteins - chemistry</subject><subject>rab GTP-Binding Proteins - isolation & purification</subject><subject>RESOLUTION</subject><subject>small Rab GTPases</subject><subject>SPACE GROUPS</subject><subject>vesicular membrane traffic</subject><issn>1744-3091</issn><issn>1744-3091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURiMEoqXlAdigSKyQCPgnjuMN0lDoFDpAxRQKbCzHY3cMjh3ZaWng5es01VBggTe2Pp_zSVc3yx5A8BRCQJ8tIS1LDBgEBJSIMXIr2x6jYsxu33hvZfdi_AYAxqyq72ZbsCIQIlpvZ7-OzoLRRoreePckl2GIvbDW_LwKcuFWeReUNa1xIgz55yKIYUP50yC6tZEJE3aIJuZe561oW2GNcPnb5bIsPoimzufHRyKq1OR7ZVwufdtZdbGb3dHCRnX_-t7JPu6_Ot47KBbv56_3ZotCEljRAtNGSk2JSodVSgFcg7KhDMCGSrbSDZCqhlUDGsLqUgMmNKhhowVjFQIK453s-dTbnTWtWknl-iAs74Jp00zcC8P__HFmzU_9OUeQAFaNBY-mAh97w6M0vZJr6Z1TsucIYVIhShL1eKLWf5UfzBZ8zADCJaMlOoeJhRMrg48xKL0RIODjbvk_u03Ow5tj_Daul5kANgE_jFXD_xv57Ms--jojSU5uMbkm9upi44rwnVcUU8JP3s15ffjpxcs5fsNP8CWNX8Cz</recordid><startdate>200602</startdate><enddate>200602</enddate><creator>Itzen, Aymelt</creator><creator>Bleimling, Nathalie</creator><creator>Ignatev, Alexander</creator><creator>Pylypenko, Olena</creator><creator>Rak, Alexey</creator><general>Blackwell Publishing Ltd</general><general>International Union of Crystallography</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><scope>OTOTI</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7073-5238</orcidid></search><sort><creationdate>200602</creationdate><title>Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian MSS4-Rab8 GTPase protein complex</title><author>Itzen, Aymelt ; Bleimling, Nathalie ; Ignatev, Alexander ; Pylypenko, Olena ; Rak, Alexey</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5167-37bccf75eeee96ee03804b7901b7c9dfb0ce816b0b5984f09af081bfa99620e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>1,2,3‐heptanetriole</topic><topic>3-heptanetriole</topic><topic>Animals</topic><topic>CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY</topic><topic>CRYSTALLIZATION</topic><topic>Crystallization Communications</topic><topic>Crystallography, X-Ray</topic><topic>CRYSTALS</topic><topic>GEF</topic><topic>GTP Phosphohydrolases - chemistry</topic><topic>GTP Phosphohydrolases - isolation & purification</topic><topic>Guanine Nucleotide Exchange Factors - chemistry</topic><topic>Guanine Nucleotide Exchange Factors - isolation & purification</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>MEMBRANES</topic><topic>Mice</topic><topic>nucleotide release</topic><topic>PROTEINS</topic><topic>rab GTP-Binding Proteins - chemistry</topic><topic>rab GTP-Binding Proteins - isolation & purification</topic><topic>RESOLUTION</topic><topic>small Rab GTPases</topic><topic>SPACE GROUPS</topic><topic>vesicular membrane traffic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itzen, Aymelt</creatorcontrib><creatorcontrib>Bleimling, Nathalie</creatorcontrib><creatorcontrib>Ignatev, Alexander</creatorcontrib><creatorcontrib>Pylypenko, Olena</creatorcontrib><creatorcontrib>Rak, Alexey</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta crystallographica. Section F, Structural biology and crystallization communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itzen, Aymelt</au><au>Bleimling, Nathalie</au><au>Ignatev, Alexander</au><au>Pylypenko, Olena</au><au>Rak, Alexey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian MSS4-Rab8 GTPase protein complex</atitle><jtitle>Acta crystallographica. Section F, Structural biology and crystallization communications</jtitle><addtitle>Acta Cryst. F</addtitle><date>2006-02</date><risdate>2006</risdate><volume>62</volume><issue>2</issue><spage>113</spage><epage>116</epage><pages>113-116</pages><issn>1744-3091</issn><eissn>1744-3091</eissn><abstract>Rab GTPases function as ubiquitous key regulators of membrane‐vesicle transport in eukaryotic cells. MSS4 is an evolutionarily conserved protein that binds to exocytotic Rabs and facilitates nucleotide release. The MSS4 protein in complex with nucleotide‐free Rab8 GTPase has been purified and crystallized in a form suitable for structure analysis. The crystals belonged to space group P1, with unit‐cell parameters a = 40.92, b = 49.85, c = 83.48 Å, α = 102.88, β = 97.46, γ = 90.12°. A complete data set has been collected to 2 Å resolution.</abstract><cop>5 Abbey Square, Chester, Cheshire CH1 2HU, England</cop><pub>Blackwell Publishing Ltd</pub><pmid>16511278</pmid><doi>10.1107/S1744309105042995</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0001-7073-5238</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 1,2,3‐heptanetriole 3-heptanetriole Animals CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY CRYSTALLIZATION Crystallization Communications Crystallography, X-Ray CRYSTALS GEF GTP Phosphohydrolases - chemistry GTP Phosphohydrolases - isolation & purification Guanine Nucleotide Exchange Factors - chemistry Guanine Nucleotide Exchange Factors - isolation & purification Humans Life Sciences MEMBRANES Mice nucleotide release PROTEINS rab GTP-Binding Proteins - chemistry rab GTP-Binding Proteins - isolation & purification RESOLUTION small Rab GTPases SPACE GROUPS vesicular membrane traffic |
| title | Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian MSS4-Rab8 GTPase protein complex |
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