$Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii$

Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii

Galactokinase (EC 2.7.1.6) catalyzes the ATP‐dependent phosphorylation of α‐d‐galactose to α‐d‐galactose‐1‐phosphate, in an additional metabolic branch of glycolysis. The apo‐form crystal structure of the enzyme has not yet been elucidated. Crystals of galactokinase from Pyrococcus horikoshii were...

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Veröffentlicht in:	Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2006-02, Vol.62 (2), p.169-171
Hauptverfasser:	Inagaki, Eiji, Sakamoto, Keiko, Obayashi, Naomi, Terada, Takaho, Shirouzu, Mikako, Bessho, Yoshitaka, Kuroishi, Chizu, Kuramitsu, Seiki, Shinkai, Akeo, Yokoyama, Shigeyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoenzymes - biosynthesis Apoenzymes - chemistry Apoenzymes - genetics Apoenzymes - isolation & purification Archaeal Proteins - biosynthesis Archaeal Proteins - chemistry Archaeal Proteins - genetics Archaeal Proteins - isolation & purification CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY CRYSTAL STRUCTURE CRYSTALLIZATION Crystallization Communications Crystallography, X-Ray CRYSTALS Escherichia coli - enzymology Escherichia coli - genetics galactokinase Galactokinase - biosynthesis Galactokinase - chemistry Galactokinase - genetics Galactokinase - isolation & purification Gene Expression Regulation, Archaeal glycolysis Kinetics Leloir pathway LENGTH PHOSPHATES Pyrococcus horikoshii Pyrococcus horikoshii - enzymology Pyrococcus horikoshii - genetics Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification RESOLUTION SPACE GROUPS SYNCHROTRON RADIATION X-RAY DIFFRACTION
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