Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?
Enhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lympho...
Gespeichert in:
Veröffentlicht in: | British journal of cancer 1998-02, Vol.77 (4), p.614-620 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 620 |
---|---|
container_issue | 4 |
container_start_page | 614 |
container_title | British journal of cancer |
container_volume | 77 |
creator | Scott, D Barber, JBP Levine, EL Burrill, W Roberts, SA |
description | Enhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer. |
doi_str_mv | 10.1038/bjc.1998.98 |
format | Article |
fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2149942</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>9484819</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-53464b654972d8c9ef73bd323a56ebac304088c7c937748d127da1c29a909d7a3</originalsourceid><addsrcrecordid>eNp1kU2P0zAQhi0EWsrCiTPCB26Q4o-ktjmA0ArYlVZCQnC2HNtpXSV2sJOV-n_4oUxoVe0eOHk87zPv2DMIvaRkTQmX79u9XVOl5FrJR2hFG84qKpl4jFaEEFERxchT9KyUPVwVkeICXaha1pKqFfrzw7hgppBiFaKbrXd4CDanONvezwX_Sy4yRLg_DOMu2cPkQXA-TqELEBq8C9sd7rL_PftoDzh1OINtKj6WMIU7j60pCzikuMVt9qZMkIrWZzxCc3AqH8AGfCfcJUhm70IZ01Kd4qfn6Eln-uJfnM5L9Ovrl59X19Xt9283V59vKwt_nqqG15u63TS1EsxJq3wneOs446bZ-NZYTmoipRVWcSFq6SgTzlDLlFFEOWH4Jfp49B3ndvDOwruy6fWYw2DyQScT9EMlhp3epjvNaK1UzcDg7dEAJlhK9t25lhK97ErDrvSyK60k0K_utzuzp-WA_uakm2JN32WYWChnDJo2jG8Ae3fECihx67PepzlHGNR_ur4-4tFMc_ZnO2AWBIi_R1W8Dw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?</title><source>MEDLINE</source><source>Nature</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Scott, D ; Barber, JBP ; Levine, EL ; Burrill, W ; Roberts, SA</creator><creatorcontrib>Scott, D ; Barber, JBP ; Levine, EL ; Burrill, W ; Roberts, SA</creatorcontrib><description>Enhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.1998.98</identifier><identifier>PMID: 9484819</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Breast Neoplasms - blood ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cancer Research ; Cell Division ; clinical-oncology ; Confounding Factors (Epidemiology) ; Disease Susceptibility ; Drug Resistance ; Epidemiology ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; Lymphocytes - radiation effects ; Mammary gland diseases ; Medical sciences ; Micronucleus Tests - methods ; Middle Aged ; Molecular Medicine ; Oncology ; Radiation Dosage ; Reproducibility of Results ; Resting Phase, Cell Cycle - genetics ; Resting Phase, Cell Cycle - radiation effects ; Tumors</subject><ispartof>British journal of cancer, 1998-02, Vol.77 (4), p.614-620</ispartof><rights>Cancer Research Campaign 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-53464b654972d8c9ef73bd323a56ebac304088c7c937748d127da1c29a909d7a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2149942/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2149942/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,2727,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2145236$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9484819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scott, D</creatorcontrib><creatorcontrib>Barber, JBP</creatorcontrib><creatorcontrib>Levine, EL</creatorcontrib><creatorcontrib>Burrill, W</creatorcontrib><creatorcontrib>Roberts, SA</creatorcontrib><title>Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>Enhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Breast Neoplasms - blood</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer Research</subject><subject>Cell Division</subject><subject>clinical-oncology</subject><subject>Confounding Factors (Epidemiology)</subject><subject>Disease Susceptibility</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Lymphocytes - radiation effects</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Micronucleus Tests - methods</subject><subject>Middle Aged</subject><subject>Molecular Medicine</subject><subject>Oncology</subject><subject>Radiation Dosage</subject><subject>Reproducibility of Results</subject><subject>Resting Phase, Cell Cycle - genetics</subject><subject>Resting Phase, Cell Cycle - radiation effects</subject><subject>Tumors</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU2P0zAQhi0EWsrCiTPCB26Q4o-ktjmA0ArYlVZCQnC2HNtpXSV2sJOV-n_4oUxoVe0eOHk87zPv2DMIvaRkTQmX79u9XVOl5FrJR2hFG84qKpl4jFaEEFERxchT9KyUPVwVkeICXaha1pKqFfrzw7hgppBiFaKbrXd4CDanONvezwX_Sy4yRLg_DOMu2cPkQXA-TqELEBq8C9sd7rL_PftoDzh1OINtKj6WMIU7j60pCzikuMVt9qZMkIrWZzxCc3AqH8AGfCfcJUhm70IZ01Kd4qfn6Eln-uJfnM5L9Ovrl59X19Xt9283V59vKwt_nqqG15u63TS1EsxJq3wneOs446bZ-NZYTmoipRVWcSFq6SgTzlDLlFFEOWH4Jfp49B3ndvDOwruy6fWYw2DyQScT9EMlhp3epjvNaK1UzcDg7dEAJlhK9t25lhK97ErDrvSyK60k0K_utzuzp-WA_uakm2JN32WYWChnDJo2jG8Ae3fECihx67PepzlHGNR_ur4-4tFMc_ZnO2AWBIi_R1W8Dw</recordid><startdate>19980201</startdate><enddate>19980201</enddate><creator>Scott, D</creator><creator>Barber, JBP</creator><creator>Levine, EL</creator><creator>Burrill, W</creator><creator>Roberts, SA</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19980201</creationdate><title>Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?</title><author>Scott, D ; Barber, JBP ; Levine, EL ; Burrill, W ; Roberts, SA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-53464b654972d8c9ef73bd323a56ebac304088c7c937748d127da1c29a909d7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Breast Neoplasms - blood</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>Cancer Research</topic><topic>Cell Division</topic><topic>clinical-oncology</topic><topic>Confounding Factors (Epidemiology)</topic><topic>Disease Susceptibility</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Lymphocytes - radiation effects</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Micronucleus Tests - methods</topic><topic>Middle Aged</topic><topic>Molecular Medicine</topic><topic>Oncology</topic><topic>Radiation Dosage</topic><topic>Reproducibility of Results</topic><topic>Resting Phase, Cell Cycle - genetics</topic><topic>Resting Phase, Cell Cycle - radiation effects</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scott, D</creatorcontrib><creatorcontrib>Barber, JBP</creatorcontrib><creatorcontrib>Levine, EL</creatorcontrib><creatorcontrib>Burrill, W</creatorcontrib><creatorcontrib>Roberts, SA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scott, D</au><au>Barber, JBP</au><au>Levine, EL</au><au>Burrill, W</au><au>Roberts, SA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>1998-02-01</date><risdate>1998</risdate><volume>77</volume><issue>4</issue><spage>614</spage><epage>620</epage><pages>614-620</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>Enhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>9484819</pmid><doi>10.1038/bjc.1998.98</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0007-0920 |
ispartof | British journal of cancer, 1998-02, Vol.77 (4), p.614-620 |
issn | 0007-0920 1532-1827 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2149942 |
source | MEDLINE; Nature; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Adult Aged Biological and medical sciences Biomedical and Life Sciences Biomedicine Breast Neoplasms - blood Breast Neoplasms - genetics Breast Neoplasms - pathology Cancer Research Cell Division clinical-oncology Confounding Factors (Epidemiology) Disease Susceptibility Drug Resistance Epidemiology Female Gynecology. Andrology. Obstetrics Humans Lymphocytes - radiation effects Mammary gland diseases Medical sciences Micronucleus Tests - methods Middle Aged Molecular Medicine Oncology Radiation Dosage Reproducibility of Results Resting Phase, Cell Cycle - genetics Resting Phase, Cell Cycle - radiation effects Tumors |
title | Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition? |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T12%3A44%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Radiation-induced%20micronucleus%20induction%20in%20lymphocytes%20identifies%20a%20high%20frequency%20of%20radiosensitive%20cases%20among%20breast%20cancer%20patients:%20a%20test%20for%20predisposition?&rft.jtitle=British%20journal%20of%20cancer&rft.au=Scott,%20D&rft.date=1998-02-01&rft.volume=77&rft.issue=4&rft.spage=614&rft.epage=620&rft.pages=614-620&rft.issn=0007-0920&rft.eissn=1532-1827&rft.coden=BJCAAI&rft_id=info:doi/10.1038/bjc.1998.98&rft_dat=%3Cpubmed_cross%3E9484819%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/9484819&rfr_iscdi=true |