CD44 Occupancy Prevents Macrophage Multinucleation
Cells of the mononuclear phagocyte lineage have the capability to adhere to and fuse with each other and to differentiate into osteoclasts and giant cells. To investigate the macrophage adhesion/fusion mechanism, we focused our attention on CD44, a surface glycoprotein known to play a role in hemato...
Gespeichert in:
| Veröffentlicht in: | The Journal of cell biology 1998-11, Vol.143 (3), p.837-847 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 847 |
|---|---|
| container_issue | 3 |
| container_start_page | 837 |
| container_title | The Journal of cell biology |
| container_volume | 143 |
| creator | Sterling, Hyacinth Saginario, Charles Vignery, Agnès |
| description | Cells of the mononuclear phagocyte lineage have the capability to adhere to and fuse with each other and to differentiate into osteoclasts and giant cells. To investigate the macrophage adhesion/fusion mechanism, we focused our attention on CD44, a surface glycoprotein known to play a role in hematopoietic cell-cell adhesion. We report that CD44 expression by macrophages is highly and transiently induced by fusogenic conditions both in vitro and in vivo. We show that CD44 ligands, hyaluronic acid, chondroitin sulfates, and osteopontin prevent macrophage multinucleation. In addition, we report that the recombinant extracellular domain of CD44 binds fusing macrophages and prevents multinucleation in vitro. These data suggest that CD44 may control the mononucleated status of macrophages in tissues by virtue of mediating cell-cell interaction. |
| doi_str_mv | 10.1083/jcb.143.3.837 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2148144</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>1618980</jstor_id><sourcerecordid>1618980</sourcerecordid><originalsourceid>FETCH-LOGICAL-c527t-62127901998285bf62b45a0d9544627e5cac1ec5574bf3244370a941667845a03</originalsourceid><addsrcrecordid>eNqFkTtPwzAUhS0EgvIY2UCqGNhS7vUjdhYkVJ4SqAwwW45x2lRpUuykUv89rlqVx8Lk4Xw6PvccQk4RBgiKXU1tPkDOBmygmNwhPRQcEoUcdkkPgGKSCSoOyGEIUwDgkrN9sp8pZAjYI3R4y3l_ZG03N7Vd9l-9W7i6Df0XY30zn5ix6790VVvWna2cacumPiZ7hamCO9m8R-T9_u5t-Jg8jx6ehjfPiRVUtklKkcoMMMsUVSIvUppzYeAjE5ynVDphjUVnhZA8LxjlnEkwGcc0lWoFsiNyvfadd_nMfdgYy5tKz305M36pG1Pq30pdTvS4WWiKPN7Po8HlxsA3n50LrZ6VwbqqMrVruqBlrIPGcP-CKJExBSvHiz_gtOl8HVuIn0qQsek0QskaigWG4F2xjYygV5PpOJmOk2mm42SRP_9555bebBT1s7U-DW3jv81SVJkC9gV1h5ic</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217075256</pqid></control><display><type>article</type><title>CD44 Occupancy Prevents Macrophage Multinucleation</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Sterling, Hyacinth ; Saginario, Charles ; Vignery, Agnès</creator><creatorcontrib>Sterling, Hyacinth ; Saginario, Charles ; Vignery, Agnès</creatorcontrib><description>Cells of the mononuclear phagocyte lineage have the capability to adhere to and fuse with each other and to differentiate into osteoclasts and giant cells. To investigate the macrophage adhesion/fusion mechanism, we focused our attention on CD44, a surface glycoprotein known to play a role in hematopoietic cell-cell adhesion. We report that CD44 expression by macrophages is highly and transiently induced by fusogenic conditions both in vitro and in vivo. We show that CD44 ligands, hyaluronic acid, chondroitin sulfates, and osteopontin prevent macrophage multinucleation. In addition, we report that the recombinant extracellular domain of CD44 binds fusing macrophages and prevents multinucleation in vitro. These data suggest that CD44 may control the mononucleated status of macrophages in tissues by virtue of mediating cell-cell interaction.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.143.3.837</identifier><identifier>PMID: 9813101</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Alveolar macrophages ; Animals ; Cell Adhesion ; Cell Fusion ; Cell lines ; Cells ; Cellular biology ; COS Cells ; Cultured cells ; Giant cells ; Giant Cells - cytology ; Hyaluronan Receptors - biosynthesis ; Hyaluronan Receptors - physiology ; Hyaluronic Acid - pharmacology ; Ligands ; Macrophages ; Macrophages, Alveolar - cytology ; Macrophages, Alveolar - drug effects ; Macrophages, Alveolar - metabolism ; Molecules ; Rats ; Rats, Inbred F344 ; Rats, Sprague-Dawley ; Regular ; T lymphocytes</subject><ispartof>The Journal of cell biology, 1998-11, Vol.143 (3), p.837-847</ispartof><rights>Copyright 1998 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Nov 2, 1998</rights><rights>1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-62127901998285bf62b45a0d9544627e5cac1ec5574bf3244370a941667845a03</citedby><cites>FETCH-LOGICAL-c527t-62127901998285bf62b45a0d9544627e5cac1ec5574bf3244370a941667845a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148144/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148144/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9813101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sterling, Hyacinth</creatorcontrib><creatorcontrib>Saginario, Charles</creatorcontrib><creatorcontrib>Vignery, Agnès</creatorcontrib><title>CD44 Occupancy Prevents Macrophage Multinucleation</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Cells of the mononuclear phagocyte lineage have the capability to adhere to and fuse with each other and to differentiate into osteoclasts and giant cells. To investigate the macrophage adhesion/fusion mechanism, we focused our attention on CD44, a surface glycoprotein known to play a role in hematopoietic cell-cell adhesion. We report that CD44 expression by macrophages is highly and transiently induced by fusogenic conditions both in vitro and in vivo. We show that CD44 ligands, hyaluronic acid, chondroitin sulfates, and osteopontin prevent macrophage multinucleation. In addition, we report that the recombinant extracellular domain of CD44 binds fusing macrophages and prevents multinucleation in vitro. These data suggest that CD44 may control the mononucleated status of macrophages in tissues by virtue of mediating cell-cell interaction.</description><subject>Alveolar macrophages</subject><subject>Animals</subject><subject>Cell Adhesion</subject><subject>Cell Fusion</subject><subject>Cell lines</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>COS Cells</subject><subject>Cultured cells</subject><subject>Giant cells</subject><subject>Giant Cells - cytology</subject><subject>Hyaluronan Receptors - biosynthesis</subject><subject>Hyaluronan Receptors - physiology</subject><subject>Hyaluronic Acid - pharmacology</subject><subject>Ligands</subject><subject>Macrophages</subject><subject>Macrophages, Alveolar - cytology</subject><subject>Macrophages, Alveolar - drug effects</subject><subject>Macrophages, Alveolar - metabolism</subject><subject>Molecules</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Rats, Sprague-Dawley</subject><subject>Regular</subject><subject>T lymphocytes</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtPwzAUhS0EgvIY2UCqGNhS7vUjdhYkVJ4SqAwwW45x2lRpUuykUv89rlqVx8Lk4Xw6PvccQk4RBgiKXU1tPkDOBmygmNwhPRQcEoUcdkkPgGKSCSoOyGEIUwDgkrN9sp8pZAjYI3R4y3l_ZG03N7Vd9l-9W7i6Df0XY30zn5ix6790VVvWna2cacumPiZ7hamCO9m8R-T9_u5t-Jg8jx6ehjfPiRVUtklKkcoMMMsUVSIvUppzYeAjE5ynVDphjUVnhZA8LxjlnEkwGcc0lWoFsiNyvfadd_nMfdgYy5tKz305M36pG1Pq30pdTvS4WWiKPN7Po8HlxsA3n50LrZ6VwbqqMrVruqBlrIPGcP-CKJExBSvHiz_gtOl8HVuIn0qQsek0QskaigWG4F2xjYygV5PpOJmOk2mm42SRP_9555bebBT1s7U-DW3jv81SVJkC9gV1h5ic</recordid><startdate>19981102</startdate><enddate>19981102</enddate><creator>Sterling, Hyacinth</creator><creator>Saginario, Charles</creator><creator>Vignery, Agnès</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T5</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19981102</creationdate><title>CD44 Occupancy Prevents Macrophage Multinucleation</title><author>Sterling, Hyacinth ; Saginario, Charles ; Vignery, Agnès</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-62127901998285bf62b45a0d9544627e5cac1ec5574bf3244370a941667845a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alveolar macrophages</topic><topic>Animals</topic><topic>Cell Adhesion</topic><topic>Cell Fusion</topic><topic>Cell lines</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>COS Cells</topic><topic>Cultured cells</topic><topic>Giant cells</topic><topic>Giant Cells - cytology</topic><topic>Hyaluronan Receptors - biosynthesis</topic><topic>Hyaluronan Receptors - physiology</topic><topic>Hyaluronic Acid - pharmacology</topic><topic>Ligands</topic><topic>Macrophages</topic><topic>Macrophages, Alveolar - cytology</topic><topic>Macrophages, Alveolar - drug effects</topic><topic>Macrophages, Alveolar - metabolism</topic><topic>Molecules</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Rats, Sprague-Dawley</topic><topic>Regular</topic><topic>T lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sterling, Hyacinth</creatorcontrib><creatorcontrib>Saginario, Charles</creatorcontrib><creatorcontrib>Vignery, Agnès</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Immunology Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sterling, Hyacinth</au><au>Saginario, Charles</au><au>Vignery, Agnès</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD44 Occupancy Prevents Macrophage Multinucleation</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1998-11-02</date><risdate>1998</risdate><volume>143</volume><issue>3</issue><spage>837</spage><epage>847</epage><pages>837-847</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>Cells of the mononuclear phagocyte lineage have the capability to adhere to and fuse with each other and to differentiate into osteoclasts and giant cells. To investigate the macrophage adhesion/fusion mechanism, we focused our attention on CD44, a surface glycoprotein known to play a role in hematopoietic cell-cell adhesion. We report that CD44 expression by macrophages is highly and transiently induced by fusogenic conditions both in vitro and in vivo. We show that CD44 ligands, hyaluronic acid, chondroitin sulfates, and osteopontin prevent macrophage multinucleation. In addition, we report that the recombinant extracellular domain of CD44 binds fusing macrophages and prevents multinucleation in vitro. These data suggest that CD44 may control the mononucleated status of macrophages in tissues by virtue of mediating cell-cell interaction.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>9813101</pmid><doi>10.1083/jcb.143.3.837</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9525 |
| ispartof | The Journal of cell biology, 1998-11, Vol.143 (3), p.837-847 |
| issn | 0021-9525 1540-8140 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2148144 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Alveolar macrophages Animals Cell Adhesion Cell Fusion Cell lines Cells Cellular biology COS Cells Cultured cells Giant cells Giant Cells - cytology Hyaluronan Receptors - biosynthesis Hyaluronan Receptors - physiology Hyaluronic Acid - pharmacology Ligands Macrophages Macrophages, Alveolar - cytology Macrophages, Alveolar - drug effects Macrophages, Alveolar - metabolism Molecules Rats Rats, Inbred F344 Rats, Sprague-Dawley Regular T lymphocytes |
| title | CD44 Occupancy Prevents Macrophage Multinucleation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-11T19%3A46%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CD44%20Occupancy%20Prevents%20Macrophage%20Multinucleation&rft.jtitle=The%20Journal%20of%20cell%20biology&rft.au=Sterling,%20Hyacinth&rft.date=1998-11-02&rft.volume=143&rft.issue=3&rft.spage=837&rft.epage=847&rft.pages=837-847&rft.issn=0021-9525&rft.eissn=1540-8140&rft.coden=JCLBA3&rft_id=info:doi/10.1083/jcb.143.3.837&rft_dat=%3Cjstor_pubme%3E1618980%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217075256&rft_id=info:pmid/9813101&rft_jstor_id=1618980&rfr_iscdi=true |