Cloning, mutagenesis, and structural analysis of human pancreatic α-amylase expressed in Pichia pastoris

Human pancreatic α-amylase (HPA) was expressed in the methylotrophic yeast Pichia pastoris and two mutants (D197A and D197N) of a completely conserved active site carboxylic acid were generated. All recombinant proteins were shown by electrospray ionization mass spectrometry (ESI-MS) to be glycosyla...

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Veröffentlicht in:	Protein science 1999-03, Vol.8 (3), p.635-643
Hauptverfasser:	RYDBERG, EDWIN H., SIDHU, GARY, VO, HUNG C., HEWITT, JEFF, CÔTÉ, HÉLÈNE C.F., WANG, YILI, NUMAO, SHIN, MacGILLIVRAY, ROSS T.A., OVERALL, CHRISTOPHER M., BRAYER, GARY D., WITHERS, STEPHEN G.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - chemistry alpha-Amylases - genetics alpha-Amylases - metabolism Amino Acid Sequence Base Sequence catalysis Cloning, Molecular crystallography diabetes DNA Primers enzyme Glycosylation human pancreatic amylase Humans Kinetics Mass Spectrometry Molecular Sequence Data mutagenesis Mutagenesis, Site-Directed Pancreas - enzymology Pichia - genetics Pichia pastoris Protein Conformation
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