Rotamer strain as a determinant of protein structural specificity

We present direct evidence for a change in protein structural specificity due to hydrophobic core packing. High resolution structural analysis of a designed core variant of ubiquitin reveals that the protein is in slow exchange between two conformations. Examination of side-chain rotamers indicates...

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Veröffentlicht in:	Protein science 1999-12, Vol.8 (12), p.2598-2610
Hauptverfasser:	LAZAR, GREG A., JOHNSON, ERIC C., DESJARLAIS, JOHN R., HANDEL, TRACY M.
Format:	Artikel
Sprache:	eng
Schlagworte:	hydrophobic core Magnetic Resonance Spectroscopy Models, Molecular Molecular Sequence Data Mutation NMR packing protein design Protein Structure, Secondary rotamer specificity strain ubiquitin Ubiquitins - chemistry Ubiquitins - genetics
Online-Zugang:	Volltext
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