Thermodynamics of barnase unfolding

The thermodynamics of barnase denaturation has been studied calorimetrically over a broad range of temperature and pH. It is shown that in acidic solutions the heat denaturation of barnase is well approximated by a 2‐state transition. The heat denaturation of barnase proceeds with a significant incr...

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Veröffentlicht in:	Protein science 1994-04, Vol.3 (4), p.669-676
Hauptverfasser:	Griko, Yuri V., Makhatadze, George I., Privalov, Peter L., Hartley, Robert W.
Format:	Artikel
Sprache:	eng
Schlagworte:	barnase Enzyme Stability Escherichia coli - enzymology Hot Temperature Hydrogen Bonding Hydrogen-Ion Concentration hydrophobic interactions Protein Denaturation Protein Folding Ribonucleases - chemistry scanning microcalorimetry Thermodynamics
Online-Zugang:	Volltext
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