Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches
Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies. To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables...
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| Veröffentlicht in: | The Journal of cell biology 1998-09, Vol.142 (6), p.1501-1517 |
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| container_title | The Journal of cell biology |
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| creator | Karpova, T.S McNally, J.G Moltz, S.L Cooper, J.A |
| description | Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies. To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables and patches in yeast. The fine structure of actin cables and the manner in which cables disassemble support a model in which cables are composed of a number of overlapping actin filaments. No evidence for intrinsic polarity of cables was found. To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationships between cables and patches. Patches and cables were often associated, and their polarized distributions were highly correlated. Therefore, patches and cables do appear to depend on each other for assembly and function. Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments. In our studies, dramatic changes in actin polarization did not include changes in filamentous actin. In addition, the concentration of actin patches was relatively constant as cells grew. Therefore, cells do not have bursts of activity in which new parts of the actin cytoskeleton are created. |
| doi_str_mv | 10.1083/jcb.142.6.1501 |
| format | Article |
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To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables and patches in yeast. The fine structure of actin cables and the manner in which cables disassemble support a model in which cables are composed of a number of overlapping actin filaments. No evidence for intrinsic polarity of cables was found. To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationships between cables and patches. Patches and cables were often associated, and their polarized distributions were highly correlated. Therefore, patches and cables do appear to depend on each other for assembly and function. Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments. In our studies, dramatic changes in actin polarization did not include changes in filamentous actin. In addition, the concentration of actin patches was relatively constant as cells grew. Therefore, cells do not have bursts of activity in which new parts of the actin cytoskeleton are created.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.142.6.1501</identifier><identifier>PMID: 9744880</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>actin ; Actins - physiology ; cell cycle ; Cells ; Cellular biology ; cytochemistry ; cytoskeleton ; Cytoskeleton - physiology ; filamentous actin ; fluorescence ; heat stress ; microfilaments ; polymerization ; Regular ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - physiology ; Yeast</subject><ispartof>The Journal of cell biology, 1998-09, Vol.142 (6), p.1501-1517</ispartof><rights>Copyright 1998 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Sep 21, 1998</rights><rights>1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-a68075f3ab2861d0d3dc1d08c57e8dbd9f984ad2f72fba9b7797ff8e7bd2e0dc3</citedby><cites>FETCH-LOGICAL-c554t-a68075f3ab2861d0d3dc1d08c57e8dbd9f984ad2f72fba9b7797ff8e7bd2e0dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141765/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141765/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9744880$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karpova, T.S</creatorcontrib><creatorcontrib>McNally, J.G</creatorcontrib><creatorcontrib>Moltz, S.L</creatorcontrib><creatorcontrib>Cooper, J.A</creatorcontrib><title>Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies. To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables and patches in yeast. The fine structure of actin cables and the manner in which cables disassemble support a model in which cables are composed of a number of overlapping actin filaments. No evidence for intrinsic polarity of cables was found. To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationships between cables and patches. Patches and cables were often associated, and their polarized distributions were highly correlated. Therefore, patches and cables do appear to depend on each other for assembly and function. Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments. In our studies, dramatic changes in actin polarization did not include changes in filamentous actin. In addition, the concentration of actin patches was relatively constant as cells grew. Therefore, cells do not have bursts of activity in which new parts of the actin cytoskeleton are created.</description><subject>actin</subject><subject>Actins - physiology</subject><subject>cell cycle</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>cytochemistry</subject><subject>cytoskeleton</subject><subject>Cytoskeleton - physiology</subject><subject>filamentous actin</subject><subject>fluorescence</subject><subject>heat stress</subject><subject>microfilaments</subject><subject>polymerization</subject><subject>Regular</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Yeast</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUlvFDEQhS0ECkPgyglEiwO3aby23TkgRRGbFIkD5Gx5KWd66GkPths0_x4PPQrLhVOp9L56ZddD6CnBLcGKvd462xJO264lApN7aEUEx2tFOL6PVhhTsu4FFQ_Ro5y3GGMuOTtDZ73kXCm8QvYyZ9jZ8dCYyTdhnlwZ4tTE0JQNNKZ2U-MOJeavMEJZlAOYXC6aBKM5wnkz7HNjofwAqLCxI-RfbntT3AbyY_QgmDHDk1M9Rzfv3n65-rC-_vT-49Xl9doJwcvadApLEZixVHXEY8-8q0U5IUF56_vQK248DZIGa3orZS9DUCCtp4C9Y-fozeK7n-0OvIOpJDPqfRp2Jh10NIP-W5mGjb6N3zUlnMhOVINXJ4MUv82Qi94N2cE4mgninLVkSjHO6H9BIoVilKoKvvwH3MY5TfUKdanENQTCKtQukEsx5wTh7skE62PGumasa8a608eM68DzPz96h59CrfqzRd_mEtNvt46onh7HXyxyMFGb2zRkffOZVl9Me4LrWdlPvWi3gQ</recordid><startdate>19980921</startdate><enddate>19980921</enddate><creator>Karpova, T.S</creator><creator>McNally, J.G</creator><creator>Moltz, S.L</creator><creator>Cooper, J.A</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980921</creationdate><title>Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches</title><author>Karpova, T.S ; McNally, J.G ; Moltz, S.L ; Cooper, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-a68075f3ab2861d0d3dc1d08c57e8dbd9f984ad2f72fba9b7797ff8e7bd2e0dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>actin</topic><topic>Actins - physiology</topic><topic>cell cycle</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>cytochemistry</topic><topic>cytoskeleton</topic><topic>Cytoskeleton - physiology</topic><topic>filamentous actin</topic><topic>fluorescence</topic><topic>heat stress</topic><topic>microfilaments</topic><topic>polymerization</topic><topic>Regular</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karpova, T.S</creatorcontrib><creatorcontrib>McNally, J.G</creatorcontrib><creatorcontrib>Moltz, S.L</creatorcontrib><creatorcontrib>Cooper, J.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karpova, T.S</au><au>McNally, J.G</au><au>Moltz, S.L</au><au>Cooper, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1998-09-21</date><risdate>1998</risdate><volume>142</volume><issue>6</issue><spage>1501</spage><epage>1517</epage><pages>1501-1517</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies. To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables and patches in yeast. The fine structure of actin cables and the manner in which cables disassemble support a model in which cables are composed of a number of overlapping actin filaments. No evidence for intrinsic polarity of cables was found. To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationships between cables and patches. Patches and cables were often associated, and their polarized distributions were highly correlated. Therefore, patches and cables do appear to depend on each other for assembly and function. Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments. In our studies, dramatic changes in actin polarization did not include changes in filamentous actin. 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| ispartof | The Journal of cell biology, 1998-09, Vol.142 (6), p.1501-1517 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | actin Actins - physiology cell cycle Cells Cellular biology cytochemistry cytoskeleton Cytoskeleton - physiology filamentous actin fluorescence heat stress microfilaments polymerization Regular Saccharomyces cerevisiae Saccharomyces cerevisiae - physiology Yeast |
| title | Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches |
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