IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE: II. BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C
A variant mouse hepatitis virus MHV(C 3 H) to which cultured peritoneal macrophages from both PRI and C 3 H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C 3 H macrophage monolayers and killed both adult PRI and C 3 H mice when injec...
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Veröffentlicht in: | The Journal of experimental medicine 1970-03, Vol.131 (4), p.851-862 |
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creator | Shif, Ilan Bang, Frederik B. |
description | A variant mouse hepatitis virus MHV(C
3
H) to which cultured peritoneal macrophages from both PRI and C
3
H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C
3
H macrophage monolayers and killed both adult PRI and C
3
H mice when injected intraperitoneally. This new variant was antigenically indistinguishable from the wild type virus. While the emergence of the variant virus was delayed in the course of infecting C
3
H macrophages with large inocula of MHV(PRI), the second passage grew to a high titer in both cell types without delay. Thus, adaptation to the new host was immediate. Interference, apparently not interferon-mediated, between the two variant viruses may have been the cause for the delay in the emergence of the variant virus. The delayed destruction of C
3
H-cultured macrophages by large inocula of MHV(PRI) uniformly resulted in the emergence of MHV(C
3
H). Whether the new variant emerged as a result of a selection of a pre-existing stable mutant or was conditioned by "growth" in the resistant host was not determined. |
format | Article |
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3
H) to which cultured peritoneal macrophages from both PRI and C
3
H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C
3
H macrophage monolayers and killed both adult PRI and C
3
H mice when injected intraperitoneally. This new variant was antigenically indistinguishable from the wild type virus. While the emergence of the variant virus was delayed in the course of infecting C
3
H macrophages with large inocula of MHV(PRI), the second passage grew to a high titer in both cell types without delay. Thus, adaptation to the new host was immediate. Interference, apparently not interferon-mediated, between the two variant viruses may have been the cause for the delay in the emergence of the variant virus. The delayed destruction of C
3
H-cultured macrophages by large inocula of MHV(PRI) uniformly resulted in the emergence of MHV(C
3
H). Whether the new variant emerged as a result of a selection of a pre-existing stable mutant or was conditioned by "growth" in the resistant host was not determined.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>PMID: 4317220</identifier><language>eng</language><publisher>The Rockefeller University Press</publisher><ispartof>The Journal of experimental medicine, 1970-03, Vol.131 (4), p.851-862</ispartof><rights>Copyright © 1970 by The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138776/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138776/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,53796,53798</link.rule.ids></links><search><creatorcontrib>Shif, Ilan</creatorcontrib><creatorcontrib>Bang, Frederik B.</creatorcontrib><title>IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE: II. BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C</title><title>The Journal of experimental medicine</title><description>A variant mouse hepatitis virus MHV(C
3
H) to which cultured peritoneal macrophages from both PRI and C
3
H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C
3
H macrophage monolayers and killed both adult PRI and C
3
H mice when injected intraperitoneally. This new variant was antigenically indistinguishable from the wild type virus. While the emergence of the variant virus was delayed in the course of infecting C
3
H macrophages with large inocula of MHV(PRI), the second passage grew to a high titer in both cell types without delay. Thus, adaptation to the new host was immediate. Interference, apparently not interferon-mediated, between the two variant viruses may have been the cause for the delay in the emergence of the variant virus. The delayed destruction of C
3
H-cultured macrophages by large inocula of MHV(PRI) uniformly resulted in the emergence of MHV(C
3
H). Whether the new variant emerged as a result of a selection of a pre-existing stable mutant or was conditioned by "growth" in the resistant host was not determined.</description><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><recordid>eNqljMtKxDAUQIMoY2f0H-4PFJK0nY4bIcTb6YVpMiQZwYWEqlUr86JVwb_XhRvXrs7iHM4JS0SR8_SqyBanLOFcylRwXp6z6Ti-cS7yvJhP2CTPRCklT9g9Gbil4CyQCeiUDmQN2Aoau_EINa5VoED-J3IbD8rcQKO0s-taLdFD5WwDSzQYSKvV6g4cevJBmQANabxgZ8_tduwufzlj1xUGXafHj4dd9_TY7d-HdhuPQ79rh694aPv41-z71_hy-IxSZIuynGf_HnwD6C9UqA</recordid><startdate>19700331</startdate><enddate>19700331</enddate><creator>Shif, Ilan</creator><creator>Bang, Frederik B.</creator><general>The Rockefeller University Press</general><scope>5PM</scope></search><sort><creationdate>19700331</creationdate><title>IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE</title><author>Shif, Ilan ; Bang, Frederik B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_21387763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shif, Ilan</creatorcontrib><creatorcontrib>Bang, Frederik B.</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shif, Ilan</au><au>Bang, Frederik B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE: II. BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C</atitle><jtitle>The Journal of experimental medicine</jtitle><date>1970-03-31</date><risdate>1970</risdate><volume>131</volume><issue>4</issue><spage>851</spage><epage>862</epage><pages>851-862</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>A variant mouse hepatitis virus MHV(C
3
H) to which cultured peritoneal macrophages from both PRI and C
3
H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C
3
H macrophage monolayers and killed both adult PRI and C
3
H mice when injected intraperitoneally. This new variant was antigenically indistinguishable from the wild type virus. While the emergence of the variant virus was delayed in the course of infecting C
3
H macrophages with large inocula of MHV(PRI), the second passage grew to a high titer in both cell types without delay. Thus, adaptation to the new host was immediate. Interference, apparently not interferon-mediated, between the two variant viruses may have been the cause for the delay in the emergence of the variant virus. The delayed destruction of C
3
H-cultured macrophages by large inocula of MHV(PRI) uniformly resulted in the emergence of MHV(C
3
H). Whether the new variant emerged as a result of a selection of a pre-existing stable mutant or was conditioned by "growth" in the resistant host was not determined.</abstract><pub>The Rockefeller University Press</pub><pmid>4317220</pmid><oa>free_for_read</oa></addata></record> |
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source | PubMed Central; EZB Electronic Journals Library |
title | IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE: II. BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C |
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