IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE: II. BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C

A variant mouse hepatitis virus MHV(C 3 H) to which cultured peritoneal macrophages from both PRI and C 3 H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C 3 H macrophage monolayers and killed both adult PRI and C 3 H mice when injec...

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Veröffentlicht in:The Journal of experimental medicine 1970-03, Vol.131 (4), p.851-862
Hauptverfasser: Shif, Ilan, Bang, Frederik B.
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description A variant mouse hepatitis virus MHV(C 3 H) to which cultured peritoneal macrophages from both PRI and C 3 H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C 3 H macrophage monolayers and killed both adult PRI and C 3 H mice when injected intraperitoneally. This new variant was antigenically indistinguishable from the wild type virus. While the emergence of the variant virus was delayed in the course of infecting C 3 H macrophages with large inocula of MHV(PRI), the second passage grew to a high titer in both cell types without delay. Thus, adaptation to the new host was immediate. Interference, apparently not interferon-mediated, between the two variant viruses may have been the cause for the delay in the emergence of the variant virus. The delayed destruction of C 3 H-cultured macrophages by large inocula of MHV(PRI) uniformly resulted in the emergence of MHV(C 3 H). Whether the new variant emerged as a result of a selection of a pre-existing stable mutant or was conditioned by "growth" in the resistant host was not determined.
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BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C</title><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>Shif, Ilan ; Bang, Frederik B.</creator><creatorcontrib>Shif, Ilan ; Bang, Frederik B.</creatorcontrib><description>A variant mouse hepatitis virus MHV(C 3 H) to which cultured peritoneal macrophages from both PRI and C 3 H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C 3 H macrophage monolayers and killed both adult PRI and C 3 H mice when injected intraperitoneally. This new variant was antigenically indistinguishable from the wild type virus. While the emergence of the variant virus was delayed in the course of infecting C 3 H macrophages with large inocula of MHV(PRI), the second passage grew to a high titer in both cell types without delay. Thus, adaptation to the new host was immediate. Interference, apparently not interferon-mediated, between the two variant viruses may have been the cause for the delay in the emergence of the variant virus. The delayed destruction of C 3 H-cultured macrophages by large inocula of MHV(PRI) uniformly resulted in the emergence of MHV(C 3 H). 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The delayed destruction of C 3 H-cultured macrophages by large inocula of MHV(PRI) uniformly resulted in the emergence of MHV(C 3 H). 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BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C</atitle><jtitle>The Journal of experimental medicine</jtitle><date>1970-03-31</date><risdate>1970</risdate><volume>131</volume><issue>4</issue><spage>851</spage><epage>862</epage><pages>851-862</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><abstract>A variant mouse hepatitis virus MHV(C 3 H) to which cultured peritoneal macrophages from both PRI and C 3 H mice were susceptible was isolated from stocks of the MHV(PRI) strain of mouse hepatitis virus. It was cloned on C 3 H macrophage monolayers and killed both adult PRI and C 3 H mice when injected intraperitoneally. This new variant was antigenically indistinguishable from the wild type virus. While the emergence of the variant virus was delayed in the course of infecting C 3 H macrophages with large inocula of MHV(PRI), the second passage grew to a high titer in both cell types without delay. Thus, adaptation to the new host was immediate. 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title IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE: II. BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C
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