Dmp53 protects the Drosophila retina during a developmentally regulated DNA damage response
Ultraviolet (UV) light is absorbed by cellular proteins and DNA, promoting skin damage, aging and cancer. In this paper, we explore the UV response by cells of the Drosophila retina. We demonstrate that the retina enters a period of heightened UV sensitivity in the young developing pupa, a stage clo...
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| Veröffentlicht in: | The EMBO journal 2003-10, Vol.22 (20), p.5622-5632 |
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| creator | Cagan, Ross L Jassim, Omar W Fink, Jill L |
| description | Ultraviolet (UV) light is absorbed by cellular proteins and DNA, promoting skin damage, aging and cancer. In this paper, we explore the UV response by cells of the Drosophila retina. We demonstrate that the retina enters a period of heightened UV sensitivity in the young developing pupa, a stage closely associated with its period of normal developmental programmed cell death. Injury to irradiated cells included morphology changes and apoptotic cell death; these defects could be completely accounted for by DNA damage. Cell death, but not morphological changes, was blocked by the caspase inhibitor P35. Utilizing genetic and microarray data, we provide evidence for the central role of Hid expression and for Diap1 protein stability in controlling the UV response. In contrast, we found that Reaper had no effect on UV sensitivity. Surprisingly, Dmp53 is required to protect cells from UV‐mediated cell death, an effect attributed to its role in DNA repair. These in vivo results demonstrate that the cellular effects of DNA damage depend on the developmental status of the tissue. |
| doi_str_mv | 10.1093/emboj/cdg543 |
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In this paper, we explore the UV response by cells of the Drosophila retina. We demonstrate that the retina enters a period of heightened UV sensitivity in the young developing pupa, a stage closely associated with its period of normal developmental programmed cell death. Injury to irradiated cells included morphology changes and apoptotic cell death; these defects could be completely accounted for by DNA damage. Cell death, but not morphological changes, was blocked by the caspase inhibitor P35. Utilizing genetic and microarray data, we provide evidence for the central role of Hid expression and for Diap1 protein stability in controlling the UV response. In contrast, we found that Reaper had no effect on UV sensitivity. Surprisingly, Dmp53 is required to protect cells from UV‐mediated cell death, an effect attributed to its role in DNA repair. These in vivo results demonstrate that the cellular effects of DNA damage depend on the developmental status of the tissue.</description><identifier>ISSN: 0261-4189</identifier><identifier>ISSN: 1460-2075</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1093/emboj/cdg543</identifier><identifier>PMID: 14532134</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Apoptosis ; Deoxyribonucleic acid ; Diap1 protein ; Dmp53 ; DNA ; DNA Damage ; Drosophila ; Drosophila melanogaster ; Drosophila Proteins - physiology ; Hid protein ; In Situ Nick-End Labeling ; Microscopy, Electron, Scanning ; Mortality ; Pupa - radiation effects ; Reaper protein ; retina ; Retina - physiology ; Retina - radiation effects ; Retina - ultrastructure ; Trans-Activators - physiology ; Tumor Suppressor Protein p53 ; Ultraviolet Rays</subject><ispartof>The EMBO journal, 2003-10, Vol.22 (20), p.5622-5632</ispartof><rights>Copyright © 2003 European Molecular Biology Organization</rights><rights>Copyright Oxford University Press(England) Oct 15, 2003</rights><rights>Copyright © 2003 European Molecular Biology Organization 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5653-797382d19b98b06fa39dea431e726dbabf4be25ba42adeeab06caf4cf16d43e73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC213797/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC213797/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,1419,1435,27933,27934,45583,45584,46418,46842,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14532134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cagan, Ross L</creatorcontrib><creatorcontrib>Jassim, Omar W</creatorcontrib><creatorcontrib>Fink, Jill L</creatorcontrib><title>Dmp53 protects the Drosophila retina during a developmentally regulated DNA damage response</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>Ultraviolet (UV) light is absorbed by cellular proteins and DNA, promoting skin damage, aging and cancer. In this paper, we explore the UV response by cells of the Drosophila retina. We demonstrate that the retina enters a period of heightened UV sensitivity in the young developing pupa, a stage closely associated with its period of normal developmental programmed cell death. Injury to irradiated cells included morphology changes and apoptotic cell death; these defects could be completely accounted for by DNA damage. Cell death, but not morphological changes, was blocked by the caspase inhibitor P35. Utilizing genetic and microarray data, we provide evidence for the central role of Hid expression and for Diap1 protein stability in controlling the UV response. In contrast, we found that Reaper had no effect on UV sensitivity. Surprisingly, Dmp53 is required to protect cells from UV‐mediated cell death, an effect attributed to its role in DNA repair. These in vivo results demonstrate that the cellular effects of DNA damage depend on the developmental status of the tissue.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Deoxyribonucleic acid</subject><subject>Diap1 protein</subject><subject>Dmp53</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila Proteins - physiology</subject><subject>Hid protein</subject><subject>In Situ Nick-End Labeling</subject><subject>Microscopy, Electron, Scanning</subject><subject>Mortality</subject><subject>Pupa - radiation effects</subject><subject>Reaper protein</subject><subject>retina</subject><subject>Retina - physiology</subject><subject>Retina - radiation effects</subject><subject>Retina - ultrastructure</subject><subject>Trans-Activators - physiology</subject><subject>Tumor Suppressor Protein p53</subject><subject>Ultraviolet Rays</subject><issn>0261-4189</issn><issn>1460-2075</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkc1v1DAQxS0EosvCjSso4sCpoXZsx-sDh7ZbCtVSOIBA4mA58SSbxYmDnbTsf4_brJaPA3Cy5fd7M-N5CD0m-AXBkh5BW7jNUWlqzugdNCMsx2mGBb-LZjjLScrIQh6gByFsMMZ8Ich9dEAYpxmhbIa-LNue06T3boByCMmwhmTpXXD9urE68TA0nU7M6JuuTuIFrsC6voVu0NZuo16PVg9gkuXlcWJ0q2uIj6F3XYCH6F6lbYBHu3OOPr46-3D6Ol29O39zerxKS55zmgop6CIzRBZyUeC80lQa0IwSEFluCl1UrICMF5pl2gDoyJS6YmVFcsMoCDpHL6e6_Vi0YMo4nNdW9b5ptd8qpxv1u9I1a1W7KxVXcNN8jp7v_N59GyEMqm1CCdbqDtwYlOAiJ5iTf4JEkkgKHMFnf4AbN_ouLiEyPOM0khE6nKAy7jt4qPYTE6xuolW30aop2og__fWXP-FdlhHgE3DdWNj-tZg6e3tyIbjk0Rt9TyZfp4fRw974HzqWt3o66U0Y4Pte1v6rygUVXH26PFfZ6oKcfH6PlaQ_AM_P1mc</recordid><startdate>20031015</startdate><enddate>20031015</enddate><creator>Cagan, Ross L</creator><creator>Jassim, Omar W</creator><creator>Fink, Jill L</creator><general>John Wiley & Sons, Ltd</general><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20031015</creationdate><title>Dmp53 protects the Drosophila retina during a developmentally regulated DNA damage response</title><author>Cagan, Ross L ; Jassim, Omar W ; Fink, Jill L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5653-797382d19b98b06fa39dea431e726dbabf4be25ba42adeeab06caf4cf16d43e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Deoxyribonucleic acid</topic><topic>Diap1 protein</topic><topic>Dmp53</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila Proteins - physiology</topic><topic>Hid protein</topic><topic>In Situ Nick-End Labeling</topic><topic>Microscopy, Electron, Scanning</topic><topic>Mortality</topic><topic>Pupa - radiation effects</topic><topic>Reaper protein</topic><topic>retina</topic><topic>Retina - physiology</topic><topic>Retina - radiation effects</topic><topic>Retina - ultrastructure</topic><topic>Trans-Activators - physiology</topic><topic>Tumor Suppressor Protein p53</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cagan, Ross L</creatorcontrib><creatorcontrib>Jassim, Omar W</creatorcontrib><creatorcontrib>Fink, Jill L</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cagan, Ross L</au><au>Jassim, Omar W</au><au>Fink, Jill L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dmp53 protects the Drosophila retina during a developmentally regulated DNA damage response</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>2003-10-15</date><risdate>2003</risdate><volume>22</volume><issue>20</issue><spage>5622</spage><epage>5632</epage><pages>5622-5632</pages><issn>0261-4189</issn><issn>1460-2075</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>Ultraviolet (UV) light is absorbed by cellular proteins and DNA, promoting skin damage, aging and cancer. In this paper, we explore the UV response by cells of the Drosophila retina. We demonstrate that the retina enters a period of heightened UV sensitivity in the young developing pupa, a stage closely associated with its period of normal developmental programmed cell death. Injury to irradiated cells included morphology changes and apoptotic cell death; these defects could be completely accounted for by DNA damage. Cell death, but not morphological changes, was blocked by the caspase inhibitor P35. Utilizing genetic and microarray data, we provide evidence for the central role of Hid expression and for Diap1 protein stability in controlling the UV response. In contrast, we found that Reaper had no effect on UV sensitivity. Surprisingly, Dmp53 is required to protect cells from UV‐mediated cell death, an effect attributed to its role in DNA repair. These in vivo results demonstrate that the cellular effects of DNA damage depend on the developmental status of the tissue.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>14532134</pmid><doi>10.1093/emboj/cdg543</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Deoxyribonucleic acid Diap1 protein Dmp53 DNA DNA Damage Drosophila Drosophila melanogaster Drosophila Proteins - physiology Hid protein In Situ Nick-End Labeling Microscopy, Electron, Scanning Mortality Pupa - radiation effects Reaper protein retina Retina - physiology Retina - radiation effects Retina - ultrastructure Trans-Activators - physiology Tumor Suppressor Protein p53 Ultraviolet Rays |
| title | Dmp53 protects the Drosophila retina during a developmentally regulated DNA damage response |
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