Direct Visualization of the Translocation of the γ-Subspecies of Protein Kinase C in Living Cells Using Fusion Proteins with Green Fluorescent Protein

We expressed the γ-subspecies of protein kinase C (γ-PKC) fused with green fluorescent protein (GFP) in various cell lines and observed the movement of this fusion protein in living cells under a confocal laser scanning fluorescent microscope. γ-PKC-GFP fusion protein had enzymological properties ve...

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Veröffentlicht in:	The Journal of cell biology 1997-12, Vol.139 (6), p.1465-1476
Hauptverfasser:	Sakai, Norio, Sasaki, Keiko, Ikegaki, Natsu, Shirai, Yasuhito, Ono, Yoshitaka, Saito, Naoaki
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 Cells Amino Acid Sequence Animals Calcimycin - pharmacology Calcium - metabolism Cell membranes Cells Cellular biology CHO Cells COS Cells Cricetinae Cultured cells Cytochalasin D - pharmacology Cytosol Fluorescence Glioma Green Fluorescent Proteins Hybrid Cells Isoenzymes - biosynthesis Isoenzymes - chemistry Isoenzymes - metabolism Kinetics Luminescent Proteins - biosynthesis Luminescent Proteins - chemistry Luminescent Proteins - metabolism Mice Microscopy, Confocal Molecular biology Molecular Sequence Data Nervous system Neuroblastoma NIH 3T3 cells Phorbol esters Protein Kinase C - biosynthesis Protein Kinase C - chemistry Protein Kinase C - metabolism Protein transport Proteins Receptors, N-Methyl-D-Aspartate - physiology Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Tetradecanoylphorbol Acetate - pharmacology Transfection
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