Microtubule-Mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus
Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and im...
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| Veröffentlicht in: | The Journal of cell biology 1997-03, Vol.136 (5), p.1007-1021 |
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| container_title | The Journal of cell biology |
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| creator | Sodeik, Beate Ebersold, Melanie W. Helenius, Ari |
| description | Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end-directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus. |
| doi_str_mv | 10.1083/jcb.136.5.1007 |
| format | Article |
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Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end-directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.136.5.1007</identifier><identifier>PMID: 9060466</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Actin Cytoskeleton - drug effects ; Animals ; Antibodies ; Biological Transport ; Capsid ; Capsid - biosynthesis ; Capsid - metabolism ; Capsid Proteins ; Cell membranes ; Cell Nucleus - virology ; Cells ; Cellular biology ; Cercopithecus aethiops ; Cytochalasin D - pharmacology ; Cytoplasm - virology ; Cytosol ; Dyneins - analysis ; herpes simplex virus 1 ; Herpes viruses ; Herpesvirus 1, Human - metabolism ; Human herpesvirus 1 ; Immediate-Early Proteins - biosynthesis ; Infections ; Membranes ; Microtubules ; Microtubules - drug effects ; Microtubules - physiology ; Nocodazole - pharmacology ; Paclitaxel - pharmacology ; Vero Cells ; Viruses</subject><ispartof>The Journal of cell biology, 1997-03, Vol.136 (5), p.1007-1021</ispartof><rights>Copyright 1997 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Mar 10, 1997</rights><rights>1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-f1730b9de5ad89255cdfc122e21b3ab3f7b78862551178422af9fa29ef386b4d3</citedby><cites>FETCH-LOGICAL-c530t-f1730b9de5ad89255cdfc122e21b3ab3f7b78862551178422af9fa29ef386b4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9060466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sodeik, Beate</creatorcontrib><creatorcontrib>Ebersold, Melanie W.</creatorcontrib><creatorcontrib>Helenius, Ari</creatorcontrib><title>Microtubule-Mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. 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We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus.</description><subject>Actin Cytoskeleton - drug effects</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biological Transport</subject><subject>Capsid</subject><subject>Capsid - biosynthesis</subject><subject>Capsid - metabolism</subject><subject>Capsid Proteins</subject><subject>Cell membranes</subject><subject>Cell Nucleus - virology</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Cercopithecus aethiops</subject><subject>Cytochalasin D - pharmacology</subject><subject>Cytoplasm - virology</subject><subject>Cytosol</subject><subject>Dyneins - analysis</subject><subject>herpes simplex virus 1</subject><subject>Herpes viruses</subject><subject>Herpesvirus 1, Human - metabolism</subject><subject>Human herpesvirus 1</subject><subject>Immediate-Early Proteins - biosynthesis</subject><subject>Infections</subject><subject>Membranes</subject><subject>Microtubules</subject><subject>Microtubules - drug effects</subject><subject>Microtubules - physiology</subject><subject>Nocodazole - pharmacology</subject><subject>Paclitaxel - pharmacology</subject><subject>Vero Cells</subject><subject>Viruses</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1vFDEQxS1EFI5ASwWSRUG3F3-u1w0SOhESKQkFAdFZXq-d-LS7XvyByH-PT3cKkIZqNHo_v_HMA-AVRmuMOnq6Nf0a03bNa4vEE7DCnKGmwww9BSuECG4kJ_wZeJ7SFiHEBKPH4FiiFrG2XYHvV97EkEtfRttc2cHrbAd4E_WclhAzDA5ezCZMfr6F5zYuNsEvflpG-wt-87EkiOFGL8kPCeYA852F18WMtqQX4MjpMdmXh3oCvp59vNmcN5efP11sPlw2hlOUG4cFRb0cLNdDJwnnZnAGE2IJ7qnuqRO96Lq2ChiLjhGinXSaSOto1_ZsoCfg_d53Kf1kB2PnHPWolugnHe9V0F79q8z-Tt2Gn4pgSpiQ1eDdwSCGH8WmrCafjB1HPdtQkqrjOW_p_0HMJUOY7MC3j8BtKHGuV6hDBRJCSFyh9R6q508pWvfwZYzULllVk1U1WcXVLtn64M3fiz7ghyir_nqvb1MO8Y9bi-uSiP4GbYiouA</recordid><startdate>19970310</startdate><enddate>19970310</enddate><creator>Sodeik, Beate</creator><creator>Ebersold, Melanie W.</creator><creator>Helenius, Ari</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970310</creationdate><title>Microtubule-Mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus</title><author>Sodeik, Beate ; Ebersold, Melanie W. ; Helenius, Ari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-f1730b9de5ad89255cdfc122e21b3ab3f7b78862551178422af9fa29ef386b4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Actin Cytoskeleton - drug effects</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biological Transport</topic><topic>Capsid</topic><topic>Capsid - biosynthesis</topic><topic>Capsid - metabolism</topic><topic>Capsid Proteins</topic><topic>Cell membranes</topic><topic>Cell Nucleus - virology</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Cercopithecus aethiops</topic><topic>Cytochalasin D - pharmacology</topic><topic>Cytoplasm - virology</topic><topic>Cytosol</topic><topic>Dyneins - analysis</topic><topic>herpes simplex virus 1</topic><topic>Herpes viruses</topic><topic>Herpesvirus 1, Human - metabolism</topic><topic>Human herpesvirus 1</topic><topic>Immediate-Early Proteins - biosynthesis</topic><topic>Infections</topic><topic>Membranes</topic><topic>Microtubules</topic><topic>Microtubules - drug effects</topic><topic>Microtubules - physiology</topic><topic>Nocodazole - pharmacology</topic><topic>Paclitaxel - pharmacology</topic><topic>Vero Cells</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sodeik, Beate</creatorcontrib><creatorcontrib>Ebersold, Melanie W.</creatorcontrib><creatorcontrib>Helenius, Ari</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sodeik, Beate</au><au>Ebersold, Melanie W.</au><au>Helenius, Ari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microtubule-Mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1997-03-10</date><risdate>1997</risdate><volume>136</volume><issue>5</issue><spage>1007</spage><epage>1021</epage><pages>1007-1021</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end-directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>9060466</pmid><doi>10.1083/jcb.136.5.1007</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Actin Cytoskeleton - drug effects Animals Antibodies Biological Transport Capsid Capsid - biosynthesis Capsid - metabolism Capsid Proteins Cell membranes Cell Nucleus - virology Cells Cellular biology Cercopithecus aethiops Cytochalasin D - pharmacology Cytoplasm - virology Cytosol Dyneins - analysis herpes simplex virus 1 Herpes viruses Herpesvirus 1, Human - metabolism Human herpesvirus 1 Immediate-Early Proteins - biosynthesis Infections Membranes Microtubules Microtubules - drug effects Microtubules - physiology Nocodazole - pharmacology Paclitaxel - pharmacology Vero Cells Viruses |
| title | Microtubule-Mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus |
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