Genome-wide location of yeast RNA polymerase III transcription machinery
RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III‐transcribed) genes, the distribution of essential components of Pol III, TFIIIC and TFIIIB was mapped across the yeast...
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| Veröffentlicht in: | The EMBO journal 2003-09, Vol.22 (18), p.4738-4747 |
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| creator | Lefebvre, Olivier Harismendy, Olivier Gendrel, Christiane-Gabrielle Soularue, Pascal Gidrol, Xavier Sentenac, André Werner, Michel |
| description | RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III‐transcribed) genes, the distribution of essential components of Pol III, TFIIIC and TFIIIB was mapped across the yeast genome. During active growth, most class III genes and few additional loci were targeted by TFIIIC, TFIIIB and Pol III, indicating that they were transcriptionally active.
SNR52
, which encodes a snoRNA, was identified as a new class III gene. During the late growth phase, TFIIIC remained bound to most class III genes while the recruitment of Pol III and, to a lesser extent, of TFIIIB was down regulated. This study fixes a reasonable upper bound to the number of class III genes in yeast and points to a global regulation at the level of Pol III and TFIIIB recruitment. |
| doi_str_mv | 10.1093/emboj/cdg466 |
| format | Article |
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SNR52
, which encodes a snoRNA, was identified as a new class III gene. During the late growth phase, TFIIIC remained bound to most class III genes while the recruitment of Pol III and, to a lesser extent, of TFIIIB was down regulated. This study fixes a reasonable upper bound to the number of class III genes in yeast and points to a global regulation at the level of Pol III and TFIIIB recruitment.</description><identifier>ISSN: 0261-4189</identifier><identifier>ISSN: 1460-2075</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1093/emboj/cdg466</identifier><identifier>PMID: 12970186</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Base Sequence ; ChIP on chip ; Chromosome Mapping ; Chromosomes, Fungal ; EMBO09 ; EMBO43 ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Fungal ; Genome, Fungal ; microarray ; Molecular Sequence Data ; PolIII protein ; RNA Polymerase III - genetics ; RNA, Fungal - classification ; RNA, Fungal - genetics ; rRNA 5S ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; transcription ; Transcription Factors, TFIII - metabolism ; Transcription, Genetic ; tRNA gene ; yeast ; Yeasts</subject><ispartof>The EMBO journal, 2003-09, Vol.22 (18), p.4738-4747</ispartof><rights>European Molecular Biology Organization 2003</rights><rights>Copyright © 2003 European Molecular Biology Organization</rights><rights>Copyright Oxford University Press(England) Sep 15, 2003</rights><rights>Copyright © 2003 European Molecular Biology Organization 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6035-ac35195a4db891fe2e48350941af61e1cd4a4fd97edeb07117329094351c88833</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC212732/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC212732/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12970186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lefebvre, Olivier</creatorcontrib><creatorcontrib>Harismendy, Olivier</creatorcontrib><creatorcontrib>Gendrel, Christiane-Gabrielle</creatorcontrib><creatorcontrib>Soularue, Pascal</creatorcontrib><creatorcontrib>Gidrol, Xavier</creatorcontrib><creatorcontrib>Sentenac, André</creatorcontrib><creatorcontrib>Werner, Michel</creatorcontrib><title>Genome-wide location of yeast RNA polymerase III transcription machinery</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III‐transcribed) genes, the distribution of essential components of Pol III, TFIIIC and TFIIIB was mapped across the yeast genome. During active growth, most class III genes and few additional loci were targeted by TFIIIC, TFIIIB and Pol III, indicating that they were transcriptionally active.
SNR52
, which encodes a snoRNA, was identified as a new class III gene. During the late growth phase, TFIIIC remained bound to most class III genes while the recruitment of Pol III and, to a lesser extent, of TFIIIB was down regulated. This study fixes a reasonable upper bound to the number of class III genes in yeast and points to a global regulation at the level of Pol III and TFIIIB recruitment.</description><subject>Base Sequence</subject><subject>ChIP on chip</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Fungal</subject><subject>EMBO09</subject><subject>EMBO43</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genome, Fungal</subject><subject>microarray</subject><subject>Molecular Sequence Data</subject><subject>PolIII protein</subject><subject>RNA Polymerase III - genetics</subject><subject>RNA, Fungal - classification</subject><subject>RNA, Fungal - genetics</subject><subject>rRNA 5S</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>transcription</subject><subject>Transcription Factors, TFIII - metabolism</subject><subject>Transcription, Genetic</subject><subject>tRNA gene</subject><subject>yeast</subject><subject>Yeasts</subject><issn>0261-4189</issn><issn>1460-2075</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc2P0zAQxS0EYkvhxhUUceBEWDuO7eTAYSlLt2gpEgL1aLnOpOuS2MVOWPLfYzZVtyA-Tj7M7z2_mYfQY4JfElzSU2jXbnuqq03O-R00ITnHaYYFu4smOOMkzUlRnqAHIWwxxqwQ5D46IVkpMCn4BF3MwboW0mtTQdI4rTrjbOLqZAAVuuTj8izZuWZowasAyWKxSDqvbNDe7G7IVukrY8EPD9G9WjUBHu3fKfr89vzT7CK9_DBfzM4uU80xZanSlJGSqbxaFyWpIYO8oAyXOVE1J0B0lau8rkoBFayxIETQrIzjqNJFUVA6Ra9G312_bqHSYGOgRu68aZUfpFNG_jqx5kpu3DeZkSx6Rf3zvd67rz2ETrYmaGgaZcH1QQrKaZkJ_l8wnhXjIjYwRc9-A7eu9zYeQcZNM0ZFXHGKXoyQ9i4ED_UhMcHyZ4_ypkc59hjxp8db3sL74iLARuDaNDD800yev3_9TrAyBmFRl466ECV2A_4o7J-DPBl5q7rew-GjW7-_znFx_J8JHXw_jJX_IrmIBnK1nMv56k2-oksiZ_QHO6Hdhg</recordid><startdate>20030915</startdate><enddate>20030915</enddate><creator>Lefebvre, Olivier</creator><creator>Harismendy, Olivier</creator><creator>Gendrel, Christiane-Gabrielle</creator><creator>Soularue, Pascal</creator><creator>Gidrol, Xavier</creator><creator>Sentenac, André</creator><creator>Werner, Michel</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20030915</creationdate><title>Genome-wide location of yeast RNA polymerase III transcription machinery</title><author>Lefebvre, Olivier ; Harismendy, Olivier ; Gendrel, Christiane-Gabrielle ; Soularue, Pascal ; Gidrol, Xavier ; Sentenac, André ; Werner, Michel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6035-ac35195a4db891fe2e48350941af61e1cd4a4fd97edeb07117329094351c88833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Base Sequence</topic><topic>ChIP on chip</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Fungal</topic><topic>EMBO09</topic><topic>EMBO43</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genome, Fungal</topic><topic>microarray</topic><topic>Molecular Sequence Data</topic><topic>PolIII protein</topic><topic>RNA Polymerase III - genetics</topic><topic>RNA, Fungal - classification</topic><topic>RNA, Fungal - genetics</topic><topic>rRNA 5S</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>transcription</topic><topic>Transcription Factors, TFIII - metabolism</topic><topic>Transcription, Genetic</topic><topic>tRNA gene</topic><topic>yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lefebvre, Olivier</creatorcontrib><creatorcontrib>Harismendy, Olivier</creatorcontrib><creatorcontrib>Gendrel, Christiane-Gabrielle</creatorcontrib><creatorcontrib>Soularue, Pascal</creatorcontrib><creatorcontrib>Gidrol, Xavier</creatorcontrib><creatorcontrib>Sentenac, André</creatorcontrib><creatorcontrib>Werner, Michel</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lefebvre, Olivier</au><au>Harismendy, Olivier</au><au>Gendrel, Christiane-Gabrielle</au><au>Soularue, Pascal</au><au>Gidrol, Xavier</au><au>Sentenac, André</au><au>Werner, Michel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide location of yeast RNA polymerase III transcription machinery</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>2003-09-15</date><risdate>2003</risdate><volume>22</volume><issue>18</issue><spage>4738</spage><epage>4747</epage><pages>4738-4747</pages><issn>0261-4189</issn><issn>1460-2075</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III‐transcribed) genes, the distribution of essential components of Pol III, TFIIIC and TFIIIB was mapped across the yeast genome. During active growth, most class III genes and few additional loci were targeted by TFIIIC, TFIIIB and Pol III, indicating that they were transcriptionally active.
SNR52
, which encodes a snoRNA, was identified as a new class III gene. During the late growth phase, TFIIIC remained bound to most class III genes while the recruitment of Pol III and, to a lesser extent, of TFIIIB was down regulated. This study fixes a reasonable upper bound to the number of class III genes in yeast and points to a global regulation at the level of Pol III and TFIIIB recruitment.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>12970186</pmid><doi>10.1093/emboj/cdg466</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Base Sequence ChIP on chip Chromosome Mapping Chromosomes, Fungal EMBO09 EMBO43 Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genome, Fungal microarray Molecular Sequence Data PolIII protein RNA Polymerase III - genetics RNA, Fungal - classification RNA, Fungal - genetics rRNA 5S Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics transcription Transcription Factors, TFIII - metabolism Transcription, Genetic tRNA gene yeast Yeasts |
| title | Genome-wide location of yeast RNA polymerase III transcription machinery |
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