The impact of chromosomes and centrosomes on spindle assembly as observed in living cells

We analyzed the role that chromosomes, kinetochores, and centrosomes play in spindle assembly in living grasshopper spermatocytes by reconstructing spindles lacking certain components. We used video-enhanced, polarization microscopy to distinguish the effect of each component on spindle microtubule...

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Veröffentlicht in:	The Journal of cell biology 1995-06, Vol.129 (5), p.1287-1300
Hauptverfasser:	Zhang, D. (Duke University, Durhan, NC.), Nicklas, R.B
Format:	Artikel
Sprache:	eng
Schlagworte:	ACRIDIDAE Animal cells Animal reproduction Animals Birefringence Cells Cellular biology Centrosome - physiology Centrosomes CHROMOSOME Chromosomes Chromosomes - physiology CROMOSOMAS Cytoplasm ESPERMATOZOO ESTRUCTURA CELULAR Fluorescent Antibody Technique Genes Grasshoppers Insects Kinetochores Kinetochores - physiology Male MEIOSE MEIOSIS Microscopy, Polarization Microtubules Microtubules - physiology Mitosis Mitotic spindle apparatus ORGANITE CELLULAIRE ORGANULOS CITOPLASMICOS Orthoptera Spermatocytes Spermatocytes - cytology Spermatocytes - ultrastructure SPERMATOZOIDE STRUCTURE CELLULAIRE X chromosome
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