Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae
The goal of this research is to identify and characterize the protein machinery that functions in the intracellular translocation and assembly of peroxisomal proteins in Saccharomyces cerevisiae. Several genes encoding proteins that are essential for this process have been identified previously by K...
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| Veröffentlicht in: | The Journal of cell biology 1993-12, Vol.123 (5), p.1133-1147 |
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| creator | Zhang, J.W Han, Y Lazarow, P.B |
| description | The goal of this research is to identify and characterize the protein machinery that functions in the intracellular translocation and assembly of peroxisomal proteins in Saccharomyces cerevisiae. Several genes encoding proteins that are essential for this process have been identified previously by Kunau and collaborators, but the mutant collection was incomplete. We have devised a positive selection procedure that identifies new mutants lacking peroxisomes or peroxisomal function. Immunofluorescence procedures for yeast were simplified so that these mutants could be rapidly and efficiently screened for those in which peroxisome biogenesis is impaired. With these tools, we have identified four complementation groups of peroxisome biogenesis mutants, and one group that appears to express reduced amounts of peroxisomal proteins. Two of our mutants lack recognizable peroxisomes, although they might contain peroxisomal membrane ghosts like those found in Zellweger syndrome. Two are selectively defective in packaging peroxisomal proteins and moreover show striking intracellular clustering of the peroxisomes. The distribution of mutants among complementation groups implies that the collection of peroxisome biogenesis mutants is still incomplete. With the procedures described, it should prove straightforward to isolate mutants from additional complementation groups |
| doi_str_mv | 10.1083/jcb.123.5.1133 |
| format | Article |
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Several genes encoding proteins that are essential for this process have been identified previously by Kunau and collaborators, but the mutant collection was incomplete. We have devised a positive selection procedure that identifies new mutants lacking peroxisomes or peroxisomal function. Immunofluorescence procedures for yeast were simplified so that these mutants could be rapidly and efficiently screened for those in which peroxisome biogenesis is impaired. With these tools, we have identified four complementation groups of peroxisome biogenesis mutants, and one group that appears to express reduced amounts of peroxisomal proteins. Two of our mutants lack recognizable peroxisomes, although they might contain peroxisomal membrane ghosts like those found in Zellweger syndrome. Two are selectively defective in packaging peroxisomal proteins and moreover show striking intracellular clustering of the peroxisomes. The distribution of mutants among complementation groups implies that the collection of peroxisome biogenesis mutants is still incomplete. With the procedures described, it should prove straightforward to isolate mutants from additional complementation groups</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.123.5.1133</identifier><identifier>PMID: 7902359</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>New York, NY: Rockefeller University Press</publisher><subject>Acetyl-CoA C-Acetyltransferase - isolation & purification ; Acetyl-CoA C-Acetyltransferase - metabolism ; Antibodies ; Biological and medical sciences ; Biological Transport ; Catalase - isolation & purification ; Catalase - metabolism ; Cell biology ; Cell metabolism, cell oxidation ; Cell physiology ; Cells ; Cellular biology ; Clone Cells ; Complementation ; Crosses, Genetic ; Enzymes ; Fatty acids ; Fluorescence ; Fluorescent Antibody Technique ; Fluorescent antibody techniques ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - metabolism ; Genetic Complementation Test ; GENETICA ; GENETIQUE ; Hydrogen Peroxide - pharmacology ; IMMUNOFLUORESCENCE ; INMUNOFLUORESCENCIA ; Microbodies - metabolism ; Microbodies - ultrastructure ; Microscopy, Immunoelectron ; Molecular and cellular biology ; MUTANT ; MUTANTES ; Mutation ; Oleic Acid ; Oleic Acids - metabolism ; Peroxisomes ; PROTEINAS ; PROTEINE ; Proteins ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae - ultrastructure ; Selection, Genetic ; Yeast ; Yeasts</subject><ispartof>The Journal of cell biology, 1993-12, Vol.123 (5), p.1133-1147</ispartof><rights>Copyright 1993 The Rockefeller University Press</rights><rights>1994 INIST-CNRS</rights><rights>Copyright Rockefeller University Press Dec 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-dd2625e99c3062d1cfbe0b97317c7a6ca7d7deee65c3c2a04b8be9c9c83eee673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3865012$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7902359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, J.W</creatorcontrib><creatorcontrib>Han, Y</creatorcontrib><creatorcontrib>Lazarow, P.B</creatorcontrib><title>Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>The goal of this research is to identify and characterize the protein machinery that functions in the intracellular translocation and assembly of peroxisomal proteins in Saccharomyces cerevisiae. Several genes encoding proteins that are essential for this process have been identified previously by Kunau and collaborators, but the mutant collection was incomplete. We have devised a positive selection procedure that identifies new mutants lacking peroxisomes or peroxisomal function. Immunofluorescence procedures for yeast were simplified so that these mutants could be rapidly and efficiently screened for those in which peroxisome biogenesis is impaired. With these tools, we have identified four complementation groups of peroxisome biogenesis mutants, and one group that appears to express reduced amounts of peroxisomal proteins. Two of our mutants lack recognizable peroxisomes, although they might contain peroxisomal membrane ghosts like those found in Zellweger syndrome. Two are selectively defective in packaging peroxisomal proteins and moreover show striking intracellular clustering of the peroxisomes. The distribution of mutants among complementation groups implies that the collection of peroxisome biogenesis mutants is still incomplete. With the procedures described, it should prove straightforward to isolate mutants from additional complementation groups</description><subject>Acetyl-CoA C-Acetyltransferase - isolation & purification</subject><subject>Acetyl-CoA C-Acetyltransferase - metabolism</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Catalase - isolation & purification</subject><subject>Catalase - metabolism</subject><subject>Cell biology</subject><subject>Cell metabolism, cell oxidation</subject><subject>Cell physiology</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Clone Cells</subject><subject>Complementation</subject><subject>Crosses, Genetic</subject><subject>Enzymes</subject><subject>Fatty acids</subject><subject>Fluorescence</subject><subject>Fluorescent Antibody Technique</subject><subject>Fluorescent antibody techniques</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - metabolism</subject><subject>Genetic Complementation Test</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>IMMUNOFLUORESCENCE</subject><subject>INMUNOFLUORESCENCIA</subject><subject>Microbodies - metabolism</subject><subject>Microbodies - ultrastructure</subject><subject>Microscopy, Immunoelectron</subject><subject>Molecular and cellular biology</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Mutation</subject><subject>Oleic Acid</subject><subject>Oleic Acids - metabolism</subject><subject>Peroxisomes</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Proteins</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae - ultrastructure</subject><subject>Selection, Genetic</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2L1DAYxoMo6zh69SAKRcRb65ukSZqLIItfsOhh3XNI07ezGdpmTNrB_e_NMMO4evEUyPN7n_fjIeQ5hYpCw99tXVtRxitRUcr5A7KiooayoTU8JCsARkstmHhMnqS0BYBa1fyCXCgNjAu9IvZb2ONQ7DCGXz6FEQs3LGnG6KdNMS6zneZU2Km7T7Q-bHDC5NOZCH1xbZ27tTGMdw5T4TDi3idv8Sl51Nsh4bPTuyY3nz7-uPxSXn3__PXyw1XpBGVz2XVMMoFaOw6SddT1LUKrFafKKSudVZ3qEFEKxx2zULdNi9pp1_DDr-Jr8v7ou1vaETuH0xztYHbRjzbemWC9-VuZ_K3ZhL1hlOpG6mzw9mQQw88F02xGnxwOg50wLMkoCQ2jQP8LUtlQYKLO4Ot_wG1Y4pSvkJsq0DXnh7mrI-RiSClifx6ZgjlEbHLEJkdshDlEnAte3V_0jJ8yzfqbk26Ts0Mf7eR8OmO8kQKy3Zq8PGLbNIf4p6mkEhhk-cVR7m0wdhOzw821rmumJOe_AbEZw7I</recordid><startdate>19931201</startdate><enddate>19931201</enddate><creator>Zhang, J.W</creator><creator>Han, Y</creator><creator>Lazarow, P.B</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19931201</creationdate><title>Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae</title><author>Zhang, J.W ; Han, Y ; Lazarow, P.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-dd2625e99c3062d1cfbe0b97317c7a6ca7d7deee65c3c2a04b8be9c9c83eee673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetyl-CoA C-Acetyltransferase - isolation & purification</topic><topic>Acetyl-CoA C-Acetyltransferase - metabolism</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Catalase - isolation & purification</topic><topic>Catalase - metabolism</topic><topic>Cell biology</topic><topic>Cell metabolism, cell oxidation</topic><topic>Cell physiology</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Clone Cells</topic><topic>Complementation</topic><topic>Crosses, Genetic</topic><topic>Enzymes</topic><topic>Fatty acids</topic><topic>Fluorescence</topic><topic>Fluorescent Antibody Technique</topic><topic>Fluorescent antibody techniques</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - metabolism</topic><topic>Genetic Complementation Test</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>IMMUNOFLUORESCENCE</topic><topic>INMUNOFLUORESCENCIA</topic><topic>Microbodies - metabolism</topic><topic>Microbodies - ultrastructure</topic><topic>Microscopy, Immunoelectron</topic><topic>Molecular and cellular biology</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Mutation</topic><topic>Oleic Acid</topic><topic>Oleic Acids - metabolism</topic><topic>Peroxisomes</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Proteins</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae - ultrastructure</topic><topic>Selection, Genetic</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, J.W</creatorcontrib><creatorcontrib>Han, Y</creatorcontrib><creatorcontrib>Lazarow, P.B</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, J.W</au><au>Han, Y</au><au>Lazarow, P.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>123</volume><issue>5</issue><spage>1133</spage><epage>1147</epage><pages>1133-1147</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>The goal of this research is to identify and characterize the protein machinery that functions in the intracellular translocation and assembly of peroxisomal proteins in Saccharomyces cerevisiae. Several genes encoding proteins that are essential for this process have been identified previously by Kunau and collaborators, but the mutant collection was incomplete. We have devised a positive selection procedure that identifies new mutants lacking peroxisomes or peroxisomal function. Immunofluorescence procedures for yeast were simplified so that these mutants could be rapidly and efficiently screened for those in which peroxisome biogenesis is impaired. With these tools, we have identified four complementation groups of peroxisome biogenesis mutants, and one group that appears to express reduced amounts of peroxisomal proteins. Two of our mutants lack recognizable peroxisomes, although they might contain peroxisomal membrane ghosts like those found in Zellweger syndrome. Two are selectively defective in packaging peroxisomal proteins and moreover show striking intracellular clustering of the peroxisomes. The distribution of mutants among complementation groups implies that the collection of peroxisome biogenesis mutants is still incomplete. With the procedures described, it should prove straightforward to isolate mutants from additional complementation groups</abstract><cop>New York, NY</cop><pub>Rockefeller University Press</pub><pmid>7902359</pmid><doi>10.1083/jcb.123.5.1133</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetyl-CoA C-Acetyltransferase - isolation & purification Acetyl-CoA C-Acetyltransferase - metabolism Antibodies Biological and medical sciences Biological Transport Catalase - isolation & purification Catalase - metabolism Cell biology Cell metabolism, cell oxidation Cell physiology Cells Cellular biology Clone Cells Complementation Crosses, Genetic Enzymes Fatty acids Fluorescence Fluorescent Antibody Technique Fluorescent antibody techniques Fundamental and applied biological sciences. Psychology Fungal Proteins - metabolism Genetic Complementation Test GENETICA GENETIQUE Hydrogen Peroxide - pharmacology IMMUNOFLUORESCENCE INMUNOFLUORESCENCIA Microbodies - metabolism Microbodies - ultrastructure Microscopy, Immunoelectron Molecular and cellular biology MUTANT MUTANTES Mutation Oleic Acid Oleic Acids - metabolism Peroxisomes PROTEINAS PROTEINE Proteins SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - ultrastructure Selection, Genetic Yeast Yeasts |
| title | Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae |
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