Multiple GTP-Binding Proteins Participate in Clathrin-Coated Vesicle-Mediated Endocytosis
We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s...
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| Veröffentlicht in: | The Journal of cell biology 1993-01, Vol.120 (1), p.37-45 |
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| container_title | The Journal of cell biology |
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| creator | Carter, Laura L. Redelmeier, Thomas E. Woollenweber, Leslie A. Schmid, Sandra L. |
| description | We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTPγS, A1 F4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, A1 F4-, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits. |
| doi_str_mv | 10.1083/jcb.120.1.37 |
| format | Article |
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Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTPγS, A1 F4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, A1 F4-, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.120.1.37</identifier><identifier>PMID: 8416994</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>New York, NY: Rockefeller University Press</publisher><subject>Aluminum - pharmacology ; Aluminum Compounds ; Amino Acid Sequence ; Biochemistry ; Biological and medical sciences ; Budding ; Carrier proteins ; Cell membranes ; Cell physiology ; Cells ; Cellular biology ; Coated Pits, Cell-Membrane - physiology ; Coated vesicles ; Cytosol ; Endocytosis ; Endocytosis - drug effects ; Fluorides - pharmacology ; Fundamental and applied biological sciences. Psychology ; GTP-Binding Proteins - physiology ; Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology ; Guanosine Diphosphate - analogs & derivatives ; Guanosine Diphosphate - pharmacology ; Guanosine Triphosphate - pharmacology ; Humans ; In Vitro Techniques ; Internalization ; Molecular and cellular biology ; Molecular Sequence Data ; Peptides - chemistry ; Peptides - pharmacology ; Proteins ; Receptors, Transferrin - physiology ; Thionucleotides - pharmacology ; Tumor Cells, Cultured ; Ungulates ; Wasp Venoms - pharmacology</subject><ispartof>The Journal of cell biology, 1993-01, Vol.120 (1), p.37-45</ispartof><rights>Copyright 1992 The Rockefeller University Press</rights><rights>1993 INIST-CNRS</rights><rights>Copyright Rockefeller University Press Jan 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-4bbe8420b1e83869f4f6821aa7aef71b4a26abe01dbe1cd582b370bac26e0f2b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4602487$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8416994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carter, Laura L.</creatorcontrib><creatorcontrib>Redelmeier, Thomas E.</creatorcontrib><creatorcontrib>Woollenweber, Leslie A.</creatorcontrib><creatorcontrib>Schmid, Sandra L.</creatorcontrib><title>Multiple GTP-Binding Proteins Participate in Clathrin-Coated Vesicle-Mediated Endocytosis</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTPγS, A1 F4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, A1 F4-, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.</description><subject>Aluminum - pharmacology</subject><subject>Aluminum Compounds</subject><subject>Amino Acid Sequence</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Budding</subject><subject>Carrier proteins</subject><subject>Cell membranes</subject><subject>Cell physiology</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Coated Pits, Cell-Membrane - physiology</subject><subject>Coated vesicles</subject><subject>Cytosol</subject><subject>Endocytosis</subject><subject>Endocytosis - drug effects</subject><subject>Fluorides - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GTP-Binding Proteins - physiology</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology</subject><subject>Guanosine Diphosphate - analogs & derivatives</subject><subject>Guanosine Diphosphate - pharmacology</subject><subject>Guanosine Triphosphate - pharmacology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Internalization</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Peptides - chemistry</subject><subject>Peptides - pharmacology</subject><subject>Proteins</subject><subject>Receptors, Transferrin - physiology</subject><subject>Thionucleotides - pharmacology</subject><subject>Tumor Cells, Cultured</subject><subject>Ungulates</subject><subject>Wasp Venoms - pharmacology</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1rGzEQxUVpSZ20tx5bWEroqevqc7V7KSQmTQsJ9SEt9CQk7Wwis5YcSRvIfx-5Nu7HaZh5P97M8BB6Q_Cc4JZ9WlkzJ7Q0cyafoRkRHNct4fg5mmFMSd0JKl6i45RWGGMuOTtCRy0nTdfxGfp1PY3ZbUaoLm-W9bnzvfO31TKGDM6naqljdtZtdIbK-Wox6nwXna8XoUz66ickZ0eor6F3vwcXvg_2MYfk0iv0YtBjgtf7eoJ-fLm4WXytr75fflucXdVWUJprbgy0nGJDoGVt0w18aFpKtJYaBkkM17TRBjDpDRDbi5YaJrHRljaAh9KcoM87381k1tBb8DnqUW2iW-v4qIJ26l_Fuzt1Gx4UJaTjnSwGH_YGMdxPkLJau2RhHLWHMCUlhaBMdqKA7_8DV2GKvjxXvCTuCO9ogT7uIBtDShGGwyUEq21equSlSl6KKLZd_u7v6w_wPqCin-51naweh6i9demA8QZT3m5t3u6wVcoh_lnZECFYw54Ah-eogA</recordid><startdate>19930101</startdate><enddate>19930101</enddate><creator>Carter, Laura L.</creator><creator>Redelmeier, Thomas E.</creator><creator>Woollenweber, Leslie A.</creator><creator>Schmid, Sandra L.</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930101</creationdate><title>Multiple GTP-Binding Proteins Participate in Clathrin-Coated Vesicle-Mediated Endocytosis</title><author>Carter, Laura L. ; Redelmeier, Thomas E. ; Woollenweber, Leslie A. ; Schmid, Sandra L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-4bbe8420b1e83869f4f6821aa7aef71b4a26abe01dbe1cd582b370bac26e0f2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Aluminum - pharmacology</topic><topic>Aluminum Compounds</topic><topic>Amino Acid Sequence</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Budding</topic><topic>Carrier proteins</topic><topic>Cell membranes</topic><topic>Cell physiology</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Coated Pits, Cell-Membrane - physiology</topic><topic>Coated vesicles</topic><topic>Cytosol</topic><topic>Endocytosis</topic><topic>Endocytosis - drug effects</topic><topic>Fluorides - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GTP-Binding Proteins - physiology</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology</topic><topic>Guanosine Diphosphate - analogs & derivatives</topic><topic>Guanosine Diphosphate - pharmacology</topic><topic>Guanosine Triphosphate - pharmacology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Internalization</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Peptides - chemistry</topic><topic>Peptides - pharmacology</topic><topic>Proteins</topic><topic>Receptors, Transferrin - physiology</topic><topic>Thionucleotides - pharmacology</topic><topic>Tumor Cells, Cultured</topic><topic>Ungulates</topic><topic>Wasp Venoms - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carter, Laura L.</creatorcontrib><creatorcontrib>Redelmeier, Thomas E.</creatorcontrib><creatorcontrib>Woollenweber, Leslie A.</creatorcontrib><creatorcontrib>Schmid, Sandra L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carter, Laura L.</au><au>Redelmeier, Thomas E.</au><au>Woollenweber, Leslie A.</au><au>Schmid, Sandra L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple GTP-Binding Proteins Participate in Clathrin-Coated Vesicle-Mediated Endocytosis</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1993-01-01</date><risdate>1993</risdate><volume>120</volume><issue>1</issue><spage>37</spage><epage>45</epage><pages>37-45</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTPγS, A1 F4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, A1 F4-, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.</abstract><cop>New York, NY</cop><pub>Rockefeller University Press</pub><pmid>8416994</pmid><doi>10.1083/jcb.120.1.37</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of cell biology, 1993-01, Vol.120 (1), p.37-45 |
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| subjects | Aluminum - pharmacology Aluminum Compounds Amino Acid Sequence Biochemistry Biological and medical sciences Budding Carrier proteins Cell membranes Cell physiology Cells Cellular biology Coated Pits, Cell-Membrane - physiology Coated vesicles Cytosol Endocytosis Endocytosis - drug effects Fluorides - pharmacology Fundamental and applied biological sciences. Psychology GTP-Binding Proteins - physiology Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology Guanosine Diphosphate - analogs & derivatives Guanosine Diphosphate - pharmacology Guanosine Triphosphate - pharmacology Humans In Vitro Techniques Internalization Molecular and cellular biology Molecular Sequence Data Peptides - chemistry Peptides - pharmacology Proteins Receptors, Transferrin - physiology Thionucleotides - pharmacology Tumor Cells, Cultured Ungulates Wasp Venoms - pharmacology |
| title | Multiple GTP-Binding Proteins Participate in Clathrin-Coated Vesicle-Mediated Endocytosis |
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