Systematic Identification of Essential Genes by in vitro mariner Mutagenesis

Although the complete DNA sequences of several microbial genomes are now available, nearly 40% of the putative genes lack identifiable functions. Comprehensive screens and selections for identifying functional classes of genes are needed to convert sequence data into meaningful biological informatio...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1998-07, Vol.95 (15), p.8927-8932
Hauptverfasser:	Akerley, Brian J., Rubin, Eric J., Camilli, Andrew, Lampe, David J., Robertson, Hugh M., Mekalanos, John J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Base Sequence Biological Sciences Deoxyribonucleic acid DNA DNA Primers DNA Transposable Elements DNA-Binding Proteins Essential genes Gels Genes Genes, Bacterial Genetic transposition Haemophilus influenzae - genetics Mutagenesis Mutation Open Reading Frames Polymerase chain reaction Product category rules Streptococcus pneumoniae - genetics Transposases Transposons
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