Human Microvascular Endothelial Cells Use β 1 and β 3 Integrin Receptor Complexes to Attach to Laminin
Microvascular endothelial cells (MEC) use a set of surface receptors to adhere not only to the vascular basement membrane but, during angiogenic stimulation, to the interstitium. We examined how cultured human MEC interact with laminin-rich basement membranes. By using a panel of monoclonal antibodi...
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Veröffentlicht in: | The Journal of cell biology 1990-09, Vol.111 (3), p.1233-1243 |
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creator | Kramer, Randall H. Cheng, Yao-Fen Clyman, Ronald |
description | Microvascular endothelial cells (MEC) use a set of surface receptors to adhere not only to the vascular basement membrane but, during angiogenic stimulation, to the interstitium. We examined how cultured human MEC interact with laminin-rich basement membranes. By using a panel of monoclonal antibodies, we found that MEC cells express a number of integrin-related receptor complexes, including α 1β 1, α 2β 1,α 3β 1,α 5β 1,α 6β 1, and α vβ 3. Attachment to laminin, a major adhesive protein in basement membranes, was studied in detail. Blocking monoclonal antibodies specific to different integrin receptor complexes showed that the α 6β 1 complex was important for MEC adhesion to laminin. In addition, blocking antibody also implicated the vitronectin receptor (α vβ 3) in laminin adhesion. We used ligand affinity chromatography of detergent-solubilized receptor complexes to further define receptor specificity. On laminin-Sepharose columns, we identified several integrin receptor complexes whose affinity for the ligand was dependent on the type of divalent cation present. Several β 1 complexes, including α 1β 1,α 2β 1, and α 6β 1 bound strongly to laminin. In agreement with the antibody blocking experiments, α vβ 3 was found to bind well to laminin. However, unlike binding to its other ligands (e.g., vitronectin, fibrinogen, von Willebrand factor), α vβ 3 interaction with laminin did not appear to be Arg-Gly-Asp (RGD) sensitive. Finally, immunofluorescent staining demonstrated both β 1 and β 3 complexes in vinculin-positive focal adhesion plaques on the basal surface of MEC adhering to laminin-coated substrates. The results indicate that both these subfamilies of integrin heterodimers are involved in promoting MEC adhesion to laminin and the vascular basement membrane. |
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We examined how cultured human MEC interact with laminin-rich basement membranes. By using a panel of monoclonal antibodies, we found that MEC cells express a number of integrin-related receptor complexes, including α 1β 1, α 2β 1,α 3β 1,α 5β 1,α 6β 1, and α vβ 3. Attachment to laminin, a major adhesive protein in basement membranes, was studied in detail. Blocking monoclonal antibodies specific to different integrin receptor complexes showed that the α 6β 1 complex was important for MEC adhesion to laminin. In addition, blocking antibody also implicated the vitronectin receptor (α vβ 3) in laminin adhesion. We used ligand affinity chromatography of detergent-solubilized receptor complexes to further define receptor specificity. On laminin-Sepharose columns, we identified several integrin receptor complexes whose affinity for the ligand was dependent on the type of divalent cation present. Several β 1 complexes, including α 1β 1,α 2β 1, and α 6β 1 bound strongly to laminin. In agreement with the antibody blocking experiments, α vβ 3 was found to bind well to laminin. However, unlike binding to its other ligands (e.g., vitronectin, fibrinogen, von Willebrand factor), α vβ 3 interaction with laminin did not appear to be Arg-Gly-Asp (RGD) sensitive. Finally, immunofluorescent staining demonstrated both β 1 and β 3 complexes in vinculin-positive focal adhesion plaques on the basal surface of MEC adhering to laminin-coated substrates. The results indicate that both these subfamilies of integrin heterodimers are involved in promoting MEC adhesion to laminin and the vascular basement membrane.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.111.3.1233</identifier><identifier>PMID: 1697296</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Antibodies ; Antibodies, Monoclonal ; Basement membrane ; Basement Membrane - physiology ; Cell adhesion ; Cell Adhesion - physiology ; Collagens ; Endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - physiology ; Fibrosis ; Humans ; In Vitro Techniques ; Integrins ; Integrins - metabolism ; Laminin - metabolism ; Ligands ; Macromolecular Substances ; Microcirculation - cytology ; Monoclonal antibodies ; Protein Binding ; Receptors ; Receptors, Immunologic - metabolism ; Receptors, Vitronectin</subject><ispartof>The Journal of cell biology, 1990-09, Vol.111 (3), p.1233-1243</ispartof><rights>Copyright 1990 The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-86dffd8b3d66cc1a929dca48e8fa8bb4970ef759a21c0ea67e5904b98daad5723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1697296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kramer, Randall H.</creatorcontrib><creatorcontrib>Cheng, Yao-Fen</creatorcontrib><creatorcontrib>Clyman, Ronald</creatorcontrib><title>Human Microvascular Endothelial Cells Use β 1 and β 3 Integrin Receptor Complexes to Attach to Laminin</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Microvascular endothelial cells (MEC) use a set of surface receptors to adhere not only to the vascular basement membrane but, during angiogenic stimulation, to the interstitium. We examined how cultured human MEC interact with laminin-rich basement membranes. By using a panel of monoclonal antibodies, we found that MEC cells express a number of integrin-related receptor complexes, including α 1β 1, α 2β 1,α 3β 1,α 5β 1,α 6β 1, and α vβ 3. Attachment to laminin, a major adhesive protein in basement membranes, was studied in detail. Blocking monoclonal antibodies specific to different integrin receptor complexes showed that the α 6β 1 complex was important for MEC adhesion to laminin. In addition, blocking antibody also implicated the vitronectin receptor (α vβ 3) in laminin adhesion. We used ligand affinity chromatography of detergent-solubilized receptor complexes to further define receptor specificity. On laminin-Sepharose columns, we identified several integrin receptor complexes whose affinity for the ligand was dependent on the type of divalent cation present. Several β 1 complexes, including α 1β 1,α 2β 1, and α 6β 1 bound strongly to laminin. In agreement with the antibody blocking experiments, α vβ 3 was found to bind well to laminin. However, unlike binding to its other ligands (e.g., vitronectin, fibrinogen, von Willebrand factor), α vβ 3 interaction with laminin did not appear to be Arg-Gly-Asp (RGD) sensitive. Finally, immunofluorescent staining demonstrated both β 1 and β 3 complexes in vinculin-positive focal adhesion plaques on the basal surface of MEC adhering to laminin-coated substrates. The results indicate that both these subfamilies of integrin heterodimers are involved in promoting MEC adhesion to laminin and the vascular basement membrane.</description><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Basement membrane</subject><subject>Basement Membrane - physiology</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - physiology</subject><subject>Collagens</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - physiology</subject><subject>Fibrosis</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Integrins</subject><subject>Integrins - metabolism</subject><subject>Laminin - metabolism</subject><subject>Ligands</subject><subject>Macromolecular Substances</subject><subject>Microcirculation - cytology</subject><subject>Monoclonal antibodies</subject><subject>Protein Binding</subject><subject>Receptors</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Receptors, Vitronectin</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1q3DAUhUVpSadpt121oFV2dvVjy9KmEIY0CUwphGQtriU5o8GWppId2tfKg_SZomFCm6504Zx7dDkfQh8pqSmR_MvO9DWltOY1ZZy_QivaNqSStCGv0YoQRivVsvYtepfzjhDSdA0_QSdUqI4psULbq2WCgL97k-IDZLOMkPBFsHHeutHDiNduHDO-yw7_ecQUQ7CHgePrMLv75AO-ccbt55jwOk770f1yGc8Rn88zmO1h2sDkgw_v0ZsBxuw-PL-n6O7bxe36qtr8uLxen28qw7mcKynsMFjZcyuEMRQUU9ZAI50cQPZ9ozrihq5VwKghDkTnWkWaXkkLYNuO8VP09Zi7X_rJWePCnGDU--QnSL91BK__V4Lf6vv4oBmlgrWyBJw9B6T4c3F51pPPprQAwcUl604pIURHirE-Gkt1OSc3_P2EEn1gowsbXdhorg9sysLnl6f9sx9hFP3TUd_l0ucLmTas7D8BHbuXGw</recordid><startdate>19900901</startdate><enddate>19900901</enddate><creator>Kramer, Randall H.</creator><creator>Cheng, Yao-Fen</creator><creator>Clyman, Ronald</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900901</creationdate><title>Human Microvascular Endothelial Cells Use β 1 and β 3 Integrin Receptor Complexes to Attach to Laminin</title><author>Kramer, Randall H. ; Cheng, Yao-Fen ; Clyman, Ronald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-86dffd8b3d66cc1a929dca48e8fa8bb4970ef759a21c0ea67e5904b98daad5723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Basement membrane</topic><topic>Basement Membrane - physiology</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - physiology</topic><topic>Collagens</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - physiology</topic><topic>Fibrosis</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Integrins</topic><topic>Integrins - metabolism</topic><topic>Laminin - metabolism</topic><topic>Ligands</topic><topic>Macromolecular Substances</topic><topic>Microcirculation - cytology</topic><topic>Monoclonal antibodies</topic><topic>Protein Binding</topic><topic>Receptors</topic><topic>Receptors, Immunologic - metabolism</topic><topic>Receptors, Vitronectin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kramer, Randall H.</creatorcontrib><creatorcontrib>Cheng, Yao-Fen</creatorcontrib><creatorcontrib>Clyman, Ronald</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kramer, Randall H.</au><au>Cheng, Yao-Fen</au><au>Clyman, Ronald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Microvascular Endothelial Cells Use β 1 and β 3 Integrin Receptor Complexes to Attach to Laminin</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1990-09-01</date><risdate>1990</risdate><volume>111</volume><issue>3</issue><spage>1233</spage><epage>1243</epage><pages>1233-1243</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><abstract>Microvascular endothelial cells (MEC) use a set of surface receptors to adhere not only to the vascular basement membrane but, during angiogenic stimulation, to the interstitium. We examined how cultured human MEC interact with laminin-rich basement membranes. By using a panel of monoclonal antibodies, we found that MEC cells express a number of integrin-related receptor complexes, including α 1β 1, α 2β 1,α 3β 1,α 5β 1,α 6β 1, and α vβ 3. Attachment to laminin, a major adhesive protein in basement membranes, was studied in detail. Blocking monoclonal antibodies specific to different integrin receptor complexes showed that the α 6β 1 complex was important for MEC adhesion to laminin. In addition, blocking antibody also implicated the vitronectin receptor (α vβ 3) in laminin adhesion. We used ligand affinity chromatography of detergent-solubilized receptor complexes to further define receptor specificity. On laminin-Sepharose columns, we identified several integrin receptor complexes whose affinity for the ligand was dependent on the type of divalent cation present. Several β 1 complexes, including α 1β 1,α 2β 1, and α 6β 1 bound strongly to laminin. In agreement with the antibody blocking experiments, α vβ 3 was found to bind well to laminin. However, unlike binding to its other ligands (e.g., vitronectin, fibrinogen, von Willebrand factor), α vβ 3 interaction with laminin did not appear to be Arg-Gly-Asp (RGD) sensitive. Finally, immunofluorescent staining demonstrated both β 1 and β 3 complexes in vinculin-positive focal adhesion plaques on the basal surface of MEC adhering to laminin-coated substrates. The results indicate that both these subfamilies of integrin heterodimers are involved in promoting MEC adhesion to laminin and the vascular basement membrane.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>1697296</pmid><doi>10.1083/jcb.111.3.1233</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antibodies Antibodies, Monoclonal Basement membrane Basement Membrane - physiology Cell adhesion Cell Adhesion - physiology Collagens Endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - physiology Fibrosis Humans In Vitro Techniques Integrins Integrins - metabolism Laminin - metabolism Ligands Macromolecular Substances Microcirculation - cytology Monoclonal antibodies Protein Binding Receptors Receptors, Immunologic - metabolism Receptors, Vitronectin |
title | Human Microvascular Endothelial Cells Use β 1 and β 3 Integrin Receptor Complexes to Attach to Laminin |
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