Molecular Trapping of a Fluorescent Ceramide Analogue at the Golgi Apparatus of Fixed Cells: Interaction with Endogenous Lipids Provides a trans-Golgi Marker for Both Light and Electron Microscopy

We have previously shown that a fluorescent derivative of ceramide, N-(ε-7-nitrobenz-2-oxa-1,3-diazol-4-yl-aminocaproyl)-D-erythro-sphingosine ( C6- NBD- Cer), vitally stains the Golgi apparatus of cells (Lipsky, N. G., and R. E. Pagano. 1985. Science (Wash. DC). 228:745-747). In the present paper w...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of cell biology 1989-11, Vol.109 (5), p.2067-2079
Hauptverfasser:	Pagano, Richard E., Sepanski, Michael A., Martin, Ona C.
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Chloro-7-nitrobenzofurazan - analogs & derivatives 4-Chloro-7-nitrobenzofurazan - analysis Animal cells Animals Biological and medical sciences Cell Line Cell membranes Cell structures and functions Cells ceramide Ceramides Ceramides - analysis Cultured cells Fibroblasts Fibroblasts - enzymology Fluorescence Fundamental and applied biological sciences. Psychology Golgi apparatus Golgi Apparatus - ultrastructure Histocytochemistry Humans Indicators and Reagents Lipids Lipids - analysis Microscopy, Electron - methods Microscopy, Fluorescence - methods Molecular and cellular biology Oxadiazoles - analysis Room temperature Skin - enzymology Staining and Labeling Thiamine Pyrophosphatase - analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!