ZO-1 mRNA and Protein Expression during Tight Junction Assembly in Caco-2 Cells

We previously identified and characterized ZO-1 as a peripheral membrane protein specifically associated with the cytoplasmic surface of tight junctions. Here we describe the identification of partial cDNA sequences encoding rat and human ZO-1 and their use to study the assembly of tight junctions i...

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Veröffentlicht in:	The Journal of cell biology 1989-09, Vol.109 (3), p.1047-1056
Hauptverfasser:	Anderson, James Melvin, Van Itallie, Christina M., Peterson, Michelle D., Stevenson, Bruce R., Carew, Elizabeth A., Mooseker, Mark S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Blotting, Northern Caco 2 cells Cell Line Cell lines Cells Complementary DNA Culture Techniques - methods Cultured cells DNA - genetics DNA - isolation & purification Epithelial cells Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene expression Humans Immunoblotting Intercellular Junctions - physiology Intercellular Junctions - ultrastructure Kidney cells Liver - metabolism Liver - ultrastructure Membrane Proteins - analysis Membrane Proteins - genetics Messenger RNA Molecular and cellular biology Molecular genetics Nucleic Acid Hybridization Phosphoproteins - analysis Phosphoproteins - genetics Protein Biosynthesis Rats RNA RNA, Messenger - genetics Tight junctions Transcription, Genetic Zonula Occludens-1 Protein
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