Detection of a Specific Isoform of Alpha-Actinin with Antisera Directed against Dystrophin
We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of...
Gespeichert in:
Veröffentlicht in: | The Journal of cell biology 1989-02, Vol.108 (2), p.503-510 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 510 |
---|---|
container_issue | 2 |
container_start_page | 503 |
container_title | The Journal of cell biology |
container_volume | 108 |
creator | Hoffman, Eric P. Watkins, Simon C. Slayter, Henry S. Kunkel, Louis M. |
description | We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch-specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha-actinin. |
doi_str_mv | 10.1083/jcb.108.2.503 |
format | Article |
fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2115443</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>1613301</jstor_id><sourcerecordid>1613301</sourcerecordid><originalsourceid>FETCH-LOGICAL-c463t-850b1d27f7dbecbddd9f94b43aa58c26c8ff8840061b09531d15077b142130bd3</originalsourceid><addsrcrecordid>eNqFkTuPEzEUhS0EWkKgpAPJBaKb4OeM0yBFGx4rrUQBNDSWnxtHE3uwnUX77_EoUYCKylc-3z3XvgeAlxitMBL03d7ouViRFUf0EVhgzlAnMEOPwQIhgrs1J_wpeFbKHiHEBkavwBXpGacIL8CPravO1JAiTB4q-HVyJvhg4E1JPuXDfLsZp53qNo2KIcJfoe7gJtZQXFZwG3JrdxaqOxViqXD7UGpO0y7E5-CJV2NxL87nEnz_-OHb9efu9sunm-vNbWdYT2snONLYksEPVjujrbVrv2aaUaW4MKQ3wnshGEI91mjNKbaYo2HQmBFMkbZ0Cd6ffKejPjhrXKxZjXLK4aDyg0wqyH-VGHbyLt1LgtuuGG0Gb88GOf08ulLlIRTjxlFFl45FDkIMhDLyXxBzgonAM9idQJNTKdn5y2swknNqsqU2F5LIllrjX__9hQt9jqnpb866KkaNPqtoQrlgA6WENNcleHXC9qWm_Gdmj-ns8hu_Fam0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15212812</pqid></control><display><type>article</type><title>Detection of a Specific Isoform of Alpha-Actinin with Antisera Directed against Dystrophin</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Hoffman, Eric P. ; Watkins, Simon C. ; Slayter, Henry S. ; Kunkel, Louis M.</creator><creatorcontrib>Hoffman, Eric P. ; Watkins, Simon C. ; Slayter, Henry S. ; Kunkel, Louis M.</creatorcontrib><description>We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch-specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha-actinin.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.108.2.503</identifier><identifier>PMID: 2645301</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>New York, NY: Rockefeller University Press</publisher><subject>Actinin - analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Antibodies ; Antigens ; antisera ; Antiserum ; Biological and medical sciences ; Duchenne muscular dystrophy ; Duchenne's muscular dystrophy ; Dystrophin ; Electrophoresis, Polyacrylamide Gel ; Fast twitch muscle fibers ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Immune Sera ; Immunoblotting ; Immunology ; Mice ; Microscopy, Electron ; Miscellaneous ; Molecular Weight ; Muscle Proteins - analysis ; Muscle Proteins - immunology ; Muscles ; Muscles - analysis ; Myofibrils - analysis ; Protein isoforms ; Proteins ; Sequence Homology, Nucleic Acid ; Skeletal muscle ; Smooth muscle ; Tissue Distribution</subject><ispartof>The Journal of cell biology, 1989-02, Vol.108 (2), p.503-510</ispartof><rights>Copyright 1989 The Rockefeller University Press</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-850b1d27f7dbecbddd9f94b43aa58c26c8ff8840061b09531d15077b142130bd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7332208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2645301$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoffman, Eric P.</creatorcontrib><creatorcontrib>Watkins, Simon C.</creatorcontrib><creatorcontrib>Slayter, Henry S.</creatorcontrib><creatorcontrib>Kunkel, Louis M.</creatorcontrib><title>Detection of a Specific Isoform of Alpha-Actinin with Antisera Directed against Dystrophin</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch-specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha-actinin.</description><subject>Actinin - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>antisera</subject><subject>Antiserum</subject><subject>Biological and medical sciences</subject><subject>Duchenne muscular dystrophy</subject><subject>Duchenne's muscular dystrophy</subject><subject>Dystrophin</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fast twitch muscle fibers</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immune Sera</subject><subject>Immunoblotting</subject><subject>Immunology</subject><subject>Mice</subject><subject>Microscopy, Electron</subject><subject>Miscellaneous</subject><subject>Molecular Weight</subject><subject>Muscle Proteins - analysis</subject><subject>Muscle Proteins - immunology</subject><subject>Muscles</subject><subject>Muscles - analysis</subject><subject>Myofibrils - analysis</subject><subject>Protein isoforms</subject><subject>Proteins</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Skeletal muscle</subject><subject>Smooth muscle</subject><subject>Tissue Distribution</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTuPEzEUhS0EWkKgpAPJBaKb4OeM0yBFGx4rrUQBNDSWnxtHE3uwnUX77_EoUYCKylc-3z3XvgeAlxitMBL03d7ouViRFUf0EVhgzlAnMEOPwQIhgrs1J_wpeFbKHiHEBkavwBXpGacIL8CPravO1JAiTB4q-HVyJvhg4E1JPuXDfLsZp53qNo2KIcJfoe7gJtZQXFZwG3JrdxaqOxViqXD7UGpO0y7E5-CJV2NxL87nEnz_-OHb9efu9sunm-vNbWdYT2snONLYksEPVjujrbVrv2aaUaW4MKQ3wnshGEI91mjNKbaYo2HQmBFMkbZ0Cd6ffKejPjhrXKxZjXLK4aDyg0wqyH-VGHbyLt1LgtuuGG0Gb88GOf08ulLlIRTjxlFFl45FDkIMhDLyXxBzgonAM9idQJNTKdn5y2swknNqsqU2F5LIllrjX__9hQt9jqnpb866KkaNPqtoQrlgA6WENNcleHXC9qWm_Gdmj-ns8hu_Fam0</recordid><startdate>19890201</startdate><enddate>19890201</enddate><creator>Hoffman, Eric P.</creator><creator>Watkins, Simon C.</creator><creator>Slayter, Henry S.</creator><creator>Kunkel, Louis M.</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890201</creationdate><title>Detection of a Specific Isoform of Alpha-Actinin with Antisera Directed against Dystrophin</title><author>Hoffman, Eric P. ; Watkins, Simon C. ; Slayter, Henry S. ; Kunkel, Louis M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-850b1d27f7dbecbddd9f94b43aa58c26c8ff8840061b09531d15077b142130bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Actinin - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>antisera</topic><topic>Antiserum</topic><topic>Biological and medical sciences</topic><topic>Duchenne muscular dystrophy</topic><topic>Duchenne's muscular dystrophy</topic><topic>Dystrophin</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fast twitch muscle fibers</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immune Sera</topic><topic>Immunoblotting</topic><topic>Immunology</topic><topic>Mice</topic><topic>Microscopy, Electron</topic><topic>Miscellaneous</topic><topic>Molecular Weight</topic><topic>Muscle Proteins - analysis</topic><topic>Muscle Proteins - immunology</topic><topic>Muscles</topic><topic>Muscles - analysis</topic><topic>Myofibrils - analysis</topic><topic>Protein isoforms</topic><topic>Proteins</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Skeletal muscle</topic><topic>Smooth muscle</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoffman, Eric P.</creatorcontrib><creatorcontrib>Watkins, Simon C.</creatorcontrib><creatorcontrib>Slayter, Henry S.</creatorcontrib><creatorcontrib>Kunkel, Louis M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoffman, Eric P.</au><au>Watkins, Simon C.</au><au>Slayter, Henry S.</au><au>Kunkel, Louis M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of a Specific Isoform of Alpha-Actinin with Antisera Directed against Dystrophin</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1989-02-01</date><risdate>1989</risdate><volume>108</volume><issue>2</issue><spage>503</spage><epage>510</epage><pages>503-510</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch-specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha-actinin.</abstract><cop>New York, NY</cop><pub>Rockefeller University Press</pub><pmid>2645301</pmid><doi>10.1083/jcb.108.2.503</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0021-9525 |
ispartof | The Journal of cell biology, 1989-02, Vol.108 (2), p.503-510 |
issn | 0021-9525 1540-8140 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2115443 |
source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
subjects | Actinin - analysis Analytical, structural and metabolic biochemistry Animals Antibodies Antigens antisera Antiserum Biological and medical sciences Duchenne muscular dystrophy Duchenne's muscular dystrophy Dystrophin Electrophoresis, Polyacrylamide Gel Fast twitch muscle fibers Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Immune Sera Immunoblotting Immunology Mice Microscopy, Electron Miscellaneous Molecular Weight Muscle Proteins - analysis Muscle Proteins - immunology Muscles Muscles - analysis Myofibrils - analysis Protein isoforms Proteins Sequence Homology, Nucleic Acid Skeletal muscle Smooth muscle Tissue Distribution |
title | Detection of a Specific Isoform of Alpha-Actinin with Antisera Directed against Dystrophin |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T00%3A01%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20a%20Specific%20Isoform%20of%20Alpha-Actinin%20with%20Antisera%20Directed%20against%20Dystrophin&rft.jtitle=The%20Journal%20of%20cell%20biology&rft.au=Hoffman,%20Eric%20P.&rft.date=1989-02-01&rft.volume=108&rft.issue=2&rft.spage=503&rft.epage=510&rft.pages=503-510&rft.issn=0021-9525&rft.eissn=1540-8140&rft.coden=JCLBA3&rft_id=info:doi/10.1083/jcb.108.2.503&rft_dat=%3Cjstor_pubme%3E1613301%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15212812&rft_id=info:pmid/2645301&rft_jstor_id=1613301&rfr_iscdi=true |