Structure and Mass Analysis of 14S Dynein Obtained from Tetrahymena Cilia
Scanning transmission electron microscopic analysis revealed that the 14S fraction of Tetrahymena dynein was of a mixture of two types of particles in approximately equal proportions. The 14S dynein molecules were roughly ellipsoid in shape with approximate axes of 9.5 and 14.5 nm. About half of the...
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| Veröffentlicht in: | The Journal of cell biology 1988-01, Vol.106 (1), p.127-132 |
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| container_title | The Journal of cell biology |
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| creator | Marchese-Ragona, Silvio P. Wall, Joseph S. Johnson, Kenneth A. |
| description | Scanning transmission electron microscopic analysis revealed that the 14S fraction of Tetrahymena dynein was of a mixture of two types of particles in approximately equal proportions. The 14S dynein molecules were roughly ellipsoid in shape with approximate axes of 9.5 and 14.5 nm. About half of the particles had tails 20-24-nm long. By the integration of electron scattering intensities, particles with tails had an average mass of 510 kD with a SD of 90 kD. The globular heads of both types of particles had an average mass of 330 kD with a SD of 60 kD. The mass of the tail structure was about 180 kD. By SDS-PAGE, the 14S dynein consisted of two high molecular mass polypeptides above 300 kD that could be distinguished by immunoblot analysis. |
| doi_str_mv | 10.1083/jcb.106.1.127 |
| format | Article |
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The 14S dynein molecules were roughly ellipsoid in shape with approximate axes of 9.5 and 14.5 nm. About half of the particles had tails 20-24-nm long. By the integration of electron scattering intensities, particles with tails had an average mass of 510 kD with a SD of 90 kD. The globular heads of both types of particles had an average mass of 330 kD with a SD of 60 kD. The mass of the tail structure was about 180 kD. 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Psychology ; Immunosorbent Techniques ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Microtubules ; Molecular and cellular biology ; Molecular chains ; Molecular Weight ; Molecules ; Motility and taxis ; Particle mass ; Protein Conformation ; Tetrahymena - analysis</subject><ispartof>The Journal of cell biology, 1988-01, Vol.106 (1), p.127-132</ispartof><rights>Copyright 1988 The Rockefeller University Press</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-1d5650f271003630d0795d6e21174966846f7fd1906123e9d2cddad932ff35eb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7702664$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2963008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marchese-Ragona, Silvio P.</creatorcontrib><creatorcontrib>Wall, Joseph S.</creatorcontrib><creatorcontrib>Johnson, Kenneth A.</creatorcontrib><title>Structure and Mass Analysis of 14S Dynein Obtained from Tetrahymena Cilia</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Scanning transmission electron microscopic analysis revealed that the 14S fraction of Tetrahymena dynein was of a mixture of two types of particles in approximately equal proportions. The 14S dynein molecules were roughly ellipsoid in shape with approximate axes of 9.5 and 14.5 nm. About half of the particles had tails 20-24-nm long. By the integration of electron scattering intensities, particles with tails had an average mass of 510 kD with a SD of 90 kD. The globular heads of both types of particles had an average mass of 330 kD with a SD of 60 kD. The mass of the tail structure was about 180 kD. By SDS-PAGE, the 14S dynein consisted of two high molecular mass polypeptides above 300 kD that could be distinguished by immunoblot analysis.</description><subject>Adenosine triphosphatases</subject><subject>Adenosine Triphosphatases - analysis</subject><subject>Adenosine Triphosphatases - immunology</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Average linear density</subject><subject>Biological and medical sciences</subject><subject>Cell biology</subject><subject>Cell physiology</subject><subject>Cellulose nitrate</subject><subject>Cilia</subject><subject>Cilia - analysis</subject><subject>Dyneins - analysis</subject><subject>Dyneins - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunosorbent Techniques</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microtubules</subject><subject>Molecular and cellular biology</subject><subject>Molecular chains</subject><subject>Molecular Weight</subject><subject>Molecules</subject><subject>Motility and taxis</subject><subject>Particle mass</subject><subject>Protein Conformation</subject><subject>Tetrahymena - analysis</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtrGzEUhUVoSZ3HMrsWtAjdjXv1GGm0KQSnaQMpWSRZC1mPRGZGk0ozAf_7qti47epeOB_nHA5CFwSWBDr2ZWPX9RFLsiRUHqEFaTk0HeHwDi0AKGlUS9sP6KSUDQBwydkxOqZKMIBugW4fpjzbac4em-TwT1MKvkqm35ZY8Bgw4Q_4ept8TPh-PZmYvMMhjwN-9FM2L9vBJ4NXsY_mDL0Ppi_-fH9P0dPNt8fVj-bu_vvt6uqusZzRqSGuFS0EKgkAqyUcSNU64SkhkishOi6CDI4oEIQyrxy1zhmnGA2BtX7NTtHXne_rvB68sz7VIr1-zXEweatHE_X_Soov-nl80zWBKw7V4PPeII-_Zl8mPcRifd-b5Me5aNmBAiJFBZsdaPNYSvbhEEJA_9le1-3rIzTRdfvKf_q32YHej131y71uijV9yCbZWA6YlECF4BX7uMM2ZRrz38y6h-w69huCSZTT</recordid><startdate>19880101</startdate><enddate>19880101</enddate><creator>Marchese-Ragona, Silvio P.</creator><creator>Wall, Joseph S.</creator><creator>Johnson, Kenneth A.</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19880101</creationdate><title>Structure and Mass Analysis of 14S Dynein Obtained from Tetrahymena Cilia</title><author>Marchese-Ragona, Silvio P. ; Wall, Joseph S. ; Johnson, Kenneth A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-1d5650f271003630d0795d6e21174966846f7fd1906123e9d2cddad932ff35eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adenosine triphosphatases</topic><topic>Adenosine Triphosphatases - analysis</topic><topic>Adenosine Triphosphatases - immunology</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Average linear density</topic><topic>Biological and medical sciences</topic><topic>Cell biology</topic><topic>Cell physiology</topic><topic>Cellulose nitrate</topic><topic>Cilia</topic><topic>Cilia - analysis</topic><topic>Dyneins - analysis</topic><topic>Dyneins - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunosorbent Techniques</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microtubules</topic><topic>Molecular and cellular biology</topic><topic>Molecular chains</topic><topic>Molecular Weight</topic><topic>Molecules</topic><topic>Motility and taxis</topic><topic>Particle mass</topic><topic>Protein Conformation</topic><topic>Tetrahymena - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marchese-Ragona, Silvio P.</creatorcontrib><creatorcontrib>Wall, Joseph S.</creatorcontrib><creatorcontrib>Johnson, Kenneth A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marchese-Ragona, Silvio P.</au><au>Wall, Joseph S.</au><au>Johnson, Kenneth A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure and Mass Analysis of 14S Dynein Obtained from Tetrahymena Cilia</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1988-01-01</date><risdate>1988</risdate><volume>106</volume><issue>1</issue><spage>127</spage><epage>132</epage><pages>127-132</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>Scanning transmission electron microscopic analysis revealed that the 14S fraction of Tetrahymena dynein was of a mixture of two types of particles in approximately equal proportions. The 14S dynein molecules were roughly ellipsoid in shape with approximate axes of 9.5 and 14.5 nm. About half of the particles had tails 20-24-nm long. By the integration of electron scattering intensities, particles with tails had an average mass of 510 kD with a SD of 90 kD. The globular heads of both types of particles had an average mass of 330 kD with a SD of 60 kD. The mass of the tail structure was about 180 kD. By SDS-PAGE, the 14S dynein consisted of two high molecular mass polypeptides above 300 kD that could be distinguished by immunoblot analysis.</abstract><cop>New York, NY</cop><pub>Rockefeller University Press</pub><pmid>2963008</pmid><doi>10.1083/jcb.106.1.127</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of cell biology, 1988-01, Vol.106 (1), p.127-132 |
| issn | 0021-9525 1540-8140 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adenosine triphosphatases Adenosine Triphosphatases - analysis Adenosine Triphosphatases - immunology Animals Antibodies Average linear density Biological and medical sciences Cell biology Cell physiology Cellulose nitrate Cilia Cilia - analysis Dyneins - analysis Dyneins - immunology Fundamental and applied biological sciences. Psychology Immunosorbent Techniques Microscopy, Electron Microscopy, Electron, Scanning Microtubules Molecular and cellular biology Molecular chains Molecular Weight Molecules Motility and taxis Particle mass Protein Conformation Tetrahymena - analysis |
| title | Structure and Mass Analysis of 14S Dynein Obtained from Tetrahymena Cilia |
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