ATP-Induced Gelation-Contraction of Microtubules Assembled in vitro
We report here an ATP-dependent formation and contraction, or syneresis, of a microtubule gel using microtubule proteins prepared from calf brains. Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after ∼60 min, at which...
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| Veröffentlicht in: | The Journal of cell biology 1984-10, Vol.99 (4), p.1527-1533 |
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| container_end_page | 1533 |
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| container_issue | 4 |
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| container_title | The Journal of cell biology |
| container_volume | 99 |
| creator | Weisenberg, Richard C. Cianci, Christopher |
| description | We report here an ATP-dependent formation and contraction, or syneresis, of a microtubule gel using microtubule proteins prepared from calf brains. Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after ∼60 min, at which time the gel volume is reduced by as much as 75%. In contracted gels, microtubule bundles and aster-like structures are observable. Gelation-contraction requires only microtubule proteins present after purification by three cycles of assembly and disassembly. |
| doi_str_mv | 10.1083/jcb.99.4.1527 |
| format | Article |
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Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after ∼60 min, at which time the gel volume is reduced by as much as 75%. In contracted gels, microtubule bundles and aster-like structures are observable. Gelation-contraction requires only microtubule proteins present after purification by three cycles of assembly and disassembly.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.99.4.1527</identifier><identifier>PMID: 6480701</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>New York, NY: Rockefeller University Press</publisher><subject>Adenosine triphosphatases ; Adenosine Triphosphate - pharmacology ; Animals ; Biological and medical sciences ; Birefringence ; Brain - metabolism ; Cattle ; Cell biology ; Cell structures and functions ; Cells ; Centrifugation ; Cytoskeleton, cytoplasm. Intracellular movements ; Fundamental and applied biological sciences. 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Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after ∼60 min, at which time the gel volume is reduced by as much as 75%. In contracted gels, microtubule bundles and aster-like structures are observable. Gelation-contraction requires only microtubule proteins present after purification by three cycles of assembly and disassembly.</description><subject>Adenosine triphosphatases</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Birefringence</subject><subject>Brain - metabolism</subject><subject>Cattle</subject><subject>Cell biology</subject><subject>Cell structures and functions</subject><subject>Cells</subject><subject>Centrifugation</subject><subject>Cytoskeleton, cytoplasm. Intracellular movements</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Microscopy, Electron</subject><subject>Microtubule proteins</subject><subject>Microtubule Proteins - metabolism</subject><subject>Microtubules</subject><subject>Microtubules - drug effects</subject><subject>Microtubules - physiology</subject><subject>Microtubules - ultrastructure</subject><subject>Molecular and cellular biology</subject><subject>Neurons</subject><subject>Polymerization</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1r3DAQxUVpSDdpj7m14EPIzdsZfVjWpbAs-YKE9JCehSxLqRavlUp2IP99bHbZJqcZeD_em3mEnCEsEWr2c2ObpVJLvkRB5SeyQMGhrJHDZ7IAoFgqQcUXcpLzBgC45OyYHFe8Bgm4IOvV4-_ytm9H69ri2nVmCLEv17EfkrHzXkRf3Aeb4jA2Y-dyscrZbZtuwkNfvIQhxa_kyJsuu2_7eUr-XF0-rm_Ku4fr2_XqrrQcmSxb1VJPBSpKjcTao4SqNrZBkNgYBM5Ea7yQhnHkUqFxyDyXk-LROfDslPza-T6Pzda11s1Hdvo5ha1JrzqaoD8qffirn-KLpoiMUTYZXOwNUvw3ujzobcjWdZ3pXRyzrpGKSqgZLHfg9HfOyflDCIKeW9dT61opzfXc-sT_eH_Zgd7XPOnne91kazqfTG9DPmAKZFVXs833HbbJQ0z_MyucMil7A_8lk6U</recordid><startdate>19841001</startdate><enddate>19841001</enddate><creator>Weisenberg, Richard C.</creator><creator>Cianci, Christopher</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19841001</creationdate><title>ATP-Induced Gelation-Contraction of Microtubules Assembled in vitro</title><author>Weisenberg, Richard C. ; Cianci, Christopher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4137-d9d2f251922a718f17068acb1071ba10435daf57a3414791ae13f47a10f1ee0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Adenosine triphosphatases</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Birefringence</topic><topic>Brain - metabolism</topic><topic>Cattle</topic><topic>Cell biology</topic><topic>Cell structures and functions</topic><topic>Cells</topic><topic>Centrifugation</topic><topic>Cytoskeleton, cytoplasm. Intracellular movements</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Microscopy, Electron</topic><topic>Microtubule proteins</topic><topic>Microtubule Proteins - metabolism</topic><topic>Microtubules</topic><topic>Microtubules - drug effects</topic><topic>Microtubules - physiology</topic><topic>Microtubules - ultrastructure</topic><topic>Molecular and cellular biology</topic><topic>Neurons</topic><topic>Polymerization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weisenberg, Richard C.</creatorcontrib><creatorcontrib>Cianci, Christopher</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weisenberg, Richard C.</au><au>Cianci, Christopher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ATP-Induced Gelation-Contraction of Microtubules Assembled in vitro</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1984-10-01</date><risdate>1984</risdate><volume>99</volume><issue>4</issue><spage>1527</spage><epage>1533</epage><pages>1527-1533</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>We report here an ATP-dependent formation and contraction, or syneresis, of a microtubule gel using microtubule proteins prepared from calf brains. Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after ∼60 min, at which time the gel volume is reduced by as much as 75%. In contracted gels, microtubule bundles and aster-like structures are observable. Gelation-contraction requires only microtubule proteins present after purification by three cycles of assembly and disassembly.</abstract><cop>New York, NY</cop><pub>Rockefeller University Press</pub><pmid>6480701</pmid><doi>10.1083/jcb.99.4.1527</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9525 |
| ispartof | The Journal of cell biology, 1984-10, Vol.99 (4), p.1527-1533 |
| issn | 0021-9525 1540-8140 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2113323 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adenosine triphosphatases Adenosine Triphosphate - pharmacology Animals Biological and medical sciences Birefringence Brain - metabolism Cattle Cell biology Cell structures and functions Cells Centrifugation Cytoskeleton, cytoplasm. Intracellular movements Fundamental and applied biological sciences. Psychology Gels Microscopy, Electron Microtubule proteins Microtubule Proteins - metabolism Microtubules Microtubules - drug effects Microtubules - physiology Microtubules - ultrastructure Molecular and cellular biology Neurons Polymerization |
| title | ATP-Induced Gelation-Contraction of Microtubules Assembled in vitro |
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