Electron Microscope Histochemical Evidence for a Partial or Total Block of the Tricarboxylic Acid Cycle in the Mitochondria of Presynaptic Axon Terminals
Respiration-linked, massive accumulation of Sr2+ is used to reveal the coupled oxidation of pyruvate, α-oxoglutarate, succinate, and malate by in situ mitochondria. All of these substrates were actively oxidized in the dendritic and perikaryal mitochondria, but no α-oxoglutarate or succinate utiliza...
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| Veröffentlicht in: | The Journal of cell biology 1971-10, Vol.51 (1), p.216-222 |
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| creator | Hajós, Ferenc Kerpel-Fronius, Sándor |
| description | Respiration-linked, massive accumulation of Sr2+ is used to reveal the coupled oxidation of pyruvate, α-oxoglutarate, succinate, and malate by in situ mitochondria. All of these substrates were actively oxidized in the dendritic and perikaryal mitochondria, but no α-oxoglutarate or succinate utilization could be demonstrated in the mitochondria of the presynaptic axon terminals. A block at an early step of α-oxoglutarate and succinate oxidation is proposed to account for the negative histochemical results, since the positive reaction with pyruvate and malate proves that these mitochondria possess an intact respiratory chain and energy-coupling mechanism essential for Sr2+ accumulation. This indicates that the mitochondria in the axon terminals would be able to generate energy for synaptic function with at least some of the respiratory substrates. With regard to the block in the tricarboxylic acid cycle, the oxaloacetate necessary for citrate formation is suggested to be provided by fixation of CO2 into some of the pyruvate. |
| doi_str_mv | 10.1083/jcb.51.1.216 |
| format | Article |
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All of these substrates were actively oxidized in the dendritic and perikaryal mitochondria, but no α-oxoglutarate or succinate utilization could be demonstrated in the mitochondria of the presynaptic axon terminals. A block at an early step of α-oxoglutarate and succinate oxidation is proposed to account for the negative histochemical results, since the positive reaction with pyruvate and malate proves that these mitochondria possess an intact respiratory chain and energy-coupling mechanism essential for Sr2+ accumulation. This indicates that the mitochondria in the axon terminals would be able to generate energy for synaptic function with at least some of the respiratory substrates. With regard to the block in the tricarboxylic acid cycle, the oxaloacetate necessary for citrate formation is suggested to be provided by fixation of CO2 into some of the pyruvate.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.51.1.216</identifier><identifier>PMID: 5111875</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Axons ; Axons - metabolism ; Carbon dioxide ; Caudate Nucleus - cytology ; Cerebellar Cortex - cytology ; Cerebral Cortex - cytology ; Citric Acid Cycle ; Dendrites ; Dendrites - metabolism ; Electron microscopes ; Enzymes ; Glutarates - metabolism ; Histocytochemistry ; Malates - metabolism ; Metabolism ; Microscopy, Electron ; Mitochondria ; Mitochondria - analysis ; Mitochondria - metabolism ; Oxidation ; Oxidation-Reduction ; Pyruvates - metabolism ; Spinal Cord - cytology ; Strontium - analysis ; Strontium - metabolism ; Succinates - metabolism ; Synapses ; Thalamus - cytology ; Tricarboxylic acid cycle</subject><ispartof>The Journal of cell biology, 1971-10, Vol.51 (1), p.216-222</ispartof><rights>Copyright 1971 The Rockefeller University Press</rights><rights>Copyright © 1971 by The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-5de660d8ba896fb990dd4bec7ba4e10e3a7f14b35f7431be1bd69ef7d79d9c5e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5111875$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hajós, Ferenc</creatorcontrib><creatorcontrib>Kerpel-Fronius, Sándor</creatorcontrib><title>Electron Microscope Histochemical Evidence for a Partial or Total Block of the Tricarboxylic Acid Cycle in the Mitochondria of Presynaptic Axon Terminals</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Respiration-linked, massive accumulation of Sr2+ is used to reveal the coupled oxidation of pyruvate, α-oxoglutarate, succinate, and malate by in situ mitochondria. All of these substrates were actively oxidized in the dendritic and perikaryal mitochondria, but no α-oxoglutarate or succinate utilization could be demonstrated in the mitochondria of the presynaptic axon terminals. A block at an early step of α-oxoglutarate and succinate oxidation is proposed to account for the negative histochemical results, since the positive reaction with pyruvate and malate proves that these mitochondria possess an intact respiratory chain and energy-coupling mechanism essential for Sr2+ accumulation. This indicates that the mitochondria in the axon terminals would be able to generate energy for synaptic function with at least some of the respiratory substrates. With regard to the block in the tricarboxylic acid cycle, the oxaloacetate necessary for citrate formation is suggested to be provided by fixation of CO2 into some of the pyruvate.</description><subject>Axons</subject><subject>Axons - metabolism</subject><subject>Carbon dioxide</subject><subject>Caudate Nucleus - cytology</subject><subject>Cerebellar Cortex - cytology</subject><subject>Cerebral Cortex - cytology</subject><subject>Citric Acid Cycle</subject><subject>Dendrites</subject><subject>Dendrites - metabolism</subject><subject>Electron microscopes</subject><subject>Enzymes</subject><subject>Glutarates - metabolism</subject><subject>Histocytochemistry</subject><subject>Malates - metabolism</subject><subject>Metabolism</subject><subject>Microscopy, Electron</subject><subject>Mitochondria</subject><subject>Mitochondria - analysis</subject><subject>Mitochondria - metabolism</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Pyruvates - metabolism</subject><subject>Spinal Cord - cytology</subject><subject>Strontium - analysis</subject><subject>Strontium - metabolism</subject><subject>Succinates - metabolism</subject><subject>Synapses</subject><subject>Thalamus - cytology</subject><subject>Tricarboxylic acid cycle</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtv1DAUhS1EVYbCjiVIXrEig28Sx8kGqR1NaaVWdDGsLT9uGA9JPNiZqvNT-Ld1mFFLV36ccz9f30PIB2BzYHXxdWP0nMMc5jlUr8gMeMmyGkr2mswYyyFreM7fkLcxbhhjpSiLU3LKAaAWfEb-Ljs0Y_ADvXUm-Gj8FumVi6M3a-ydUR1d3juLg0Ha-kAVvVNhdOk6HVZ-TJuLzpvf1Ld0XCNdhVQTtH_Yd87Qc-MsXexNh9QN__RbN5H9YINTU81dwLgf1Hac3A-pjRWG3g2qi-_ISZsWfH9cz8jPy-VqcZXd_Ph-vTi_yUzJ2Jhxi1XFbK1V3VStbhpmbanRCK1KBIaFEi2UuuBt-jloBG2rBlthRWMbw7E4I98O3O1O92gNDmNQndwG16uwl145-VIZ3Fr-8vcyT9PPyzwBPh8Bwf_ZYRxl76LBrlMD-l2UNUBRiUYk45eDcRp0DNg-PQJMTlHKFKXkICGxq2T_9H9jT-Zjdkn_eNA3Ka3wzKpYVQhRPALRwqgW</recordid><startdate>19711001</startdate><enddate>19711001</enddate><creator>Hajós, Ferenc</creator><creator>Kerpel-Fronius, Sándor</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19711001</creationdate><title>Electron Microscope Histochemical Evidence for a Partial or Total Block of the Tricarboxylic Acid Cycle in the Mitochondria of Presynaptic Axon Terminals</title><author>Hajós, Ferenc ; Kerpel-Fronius, Sándor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-5de660d8ba896fb990dd4bec7ba4e10e3a7f14b35f7431be1bd69ef7d79d9c5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Axons</topic><topic>Axons - metabolism</topic><topic>Carbon dioxide</topic><topic>Caudate Nucleus - cytology</topic><topic>Cerebellar Cortex - cytology</topic><topic>Cerebral Cortex - cytology</topic><topic>Citric Acid Cycle</topic><topic>Dendrites</topic><topic>Dendrites - metabolism</topic><topic>Electron microscopes</topic><topic>Enzymes</topic><topic>Glutarates - metabolism</topic><topic>Histocytochemistry</topic><topic>Malates - metabolism</topic><topic>Metabolism</topic><topic>Microscopy, Electron</topic><topic>Mitochondria</topic><topic>Mitochondria - analysis</topic><topic>Mitochondria - metabolism</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Pyruvates - metabolism</topic><topic>Spinal Cord - cytology</topic><topic>Strontium - analysis</topic><topic>Strontium - metabolism</topic><topic>Succinates - metabolism</topic><topic>Synapses</topic><topic>Thalamus - cytology</topic><topic>Tricarboxylic acid cycle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hajós, Ferenc</creatorcontrib><creatorcontrib>Kerpel-Fronius, Sándor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hajós, Ferenc</au><au>Kerpel-Fronius, Sándor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electron Microscope Histochemical Evidence for a Partial or Total Block of the Tricarboxylic Acid Cycle in the Mitochondria of Presynaptic Axon Terminals</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1971-10-01</date><risdate>1971</risdate><volume>51</volume><issue>1</issue><spage>216</spage><epage>222</epage><pages>216-222</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><abstract>Respiration-linked, massive accumulation of Sr2+ is used to reveal the coupled oxidation of pyruvate, α-oxoglutarate, succinate, and malate by in situ mitochondria. All of these substrates were actively oxidized in the dendritic and perikaryal mitochondria, but no α-oxoglutarate or succinate utilization could be demonstrated in the mitochondria of the presynaptic axon terminals. A block at an early step of α-oxoglutarate and succinate oxidation is proposed to account for the negative histochemical results, since the positive reaction with pyruvate and malate proves that these mitochondria possess an intact respiratory chain and energy-coupling mechanism essential for Sr2+ accumulation. This indicates that the mitochondria in the axon terminals would be able to generate energy for synaptic function with at least some of the respiratory substrates. With regard to the block in the tricarboxylic acid cycle, the oxaloacetate necessary for citrate formation is suggested to be provided by fixation of CO2 into some of the pyruvate.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>5111875</pmid><doi>10.1083/jcb.51.1.216</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9525 |
| ispartof | The Journal of cell biology, 1971-10, Vol.51 (1), p.216-222 |
| issn | 0021-9525 1540-8140 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Axons Axons - metabolism Carbon dioxide Caudate Nucleus - cytology Cerebellar Cortex - cytology Cerebral Cortex - cytology Citric Acid Cycle Dendrites Dendrites - metabolism Electron microscopes Enzymes Glutarates - metabolism Histocytochemistry Malates - metabolism Metabolism Microscopy, Electron Mitochondria Mitochondria - analysis Mitochondria - metabolism Oxidation Oxidation-Reduction Pyruvates - metabolism Spinal Cord - cytology Strontium - analysis Strontium - metabolism Succinates - metabolism Synapses Thalamus - cytology Tricarboxylic acid cycle |
| title | Electron Microscope Histochemical Evidence for a Partial or Total Block of the Tricarboxylic Acid Cycle in the Mitochondria of Presynaptic Axon Terminals |
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