The Laser as a Potential Tool for Cell Research
Freshly prepared hemoglobin solutions were successively irradiated up to five times with 1 MW (monochromatic wavelength) of green (530 mμ) laser power. Oxygenated hemoglobin showed no detectable change, but the spectral absorption of reduced hemoglobin showed a shift toward the characteristic curve...
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| Veröffentlicht in: | Journal of Cell Biology (U.S.) Formerly J. Biophys. Biochem. Cytol 1965-10, Vol.27 (1), p.191-197 |
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| container_end_page | 197 |
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| container_issue | 1 |
| container_start_page | 191 |
| container_title | Journal of Cell Biology (U.S.) Formerly J. Biophys. Biochem. Cytol |
| container_volume | 27 |
| creator | Rounds, Donald E. Olson, Robert S. Johnson, Fred M. |
| description | Freshly prepared hemoglobin solutions were successively irradiated up to five times with 1 MW (monochromatic wavelength) of green (530 mμ) laser power. Oxygenated hemoglobin showed no detectable change, but the spectral absorption of reduced hemoglobin showed a shift toward the characteristic curve for the oxygenated form. Intact human erythrocytes exposed to a power density of 110 MW/cm2 of green laser radiation showed no appreciable change in diameter or mass, but they became transparent to a wavelength range from 400 to 600 mμ. A similar power density from a ruby laser failed to produce this bleaching effect. This response in the erythrocyte demonstrates a principle which suggests the laser as a tool for cell research: specific molecular components within a cell may be selectively altered by laser irradiation when an appropriate wavelength and a suitable power density are applied. |
| doi_str_mv | 10.1083/jcb.27.1.191 |
| format | Article |
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Oxygenated hemoglobin showed no detectable change, but the spectral absorption of reduced hemoglobin showed a shift toward the characteristic curve for the oxygenated form. Intact human erythrocytes exposed to a power density of 110 MW/cm2 of green laser radiation showed no appreciable change in diameter or mass, but they became transparent to a wavelength range from 400 to 600 mμ. A similar power density from a ruby laser failed to produce this bleaching effect. This response in the erythrocyte demonstrates a principle which suggests the laser as a tool for cell research: specific molecular components within a cell may be selectively altered by laser irradiation when an appropriate wavelength and a suitable power density are applied.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.27.1.191</identifier><identifier>PMID: 5857254</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>ABSORPTION ; BIOLOGY AND MEDICINE ; Cell Biology - instrumentation ; Cells ; CYTOLOGY ; DENSITY ; DIAGRAMS ; Epithelial cells ; ERYTHROCYTES ; Erythrocytes - radiation effects ; HEMOGLOBIN ; Hemoglobins ; IRRADIATION ; Laser beams ; Laser power ; Lasers ; Optical density ; RADIATION EFFECTS ; Radiation Effects on Tissues ; Ruby lasers ; SPECTRA ; USES ; VOLUME ; Wavelengths</subject><ispartof>Journal of Cell Biology (U.S.) Formerly J. 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Biophys. Biochem. Cytol</title><addtitle>J Cell Biol</addtitle><description>Freshly prepared hemoglobin solutions were successively irradiated up to five times with 1 MW (monochromatic wavelength) of green (530 mμ) laser power. Oxygenated hemoglobin showed no detectable change, but the spectral absorption of reduced hemoglobin showed a shift toward the characteristic curve for the oxygenated form. Intact human erythrocytes exposed to a power density of 110 MW/cm2 of green laser radiation showed no appreciable change in diameter or mass, but they became transparent to a wavelength range from 400 to 600 mμ. A similar power density from a ruby laser failed to produce this bleaching effect. This response in the erythrocyte demonstrates a principle which suggests the laser as a tool for cell research: specific molecular components within a cell may be selectively altered by laser irradiation when an appropriate wavelength and a suitable power density are applied.</description><subject>ABSORPTION</subject><subject>BIOLOGY AND MEDICINE</subject><subject>Cell Biology - instrumentation</subject><subject>Cells</subject><subject>CYTOLOGY</subject><subject>DENSITY</subject><subject>DIAGRAMS</subject><subject>Epithelial cells</subject><subject>ERYTHROCYTES</subject><subject>Erythrocytes - radiation effects</subject><subject>HEMOGLOBIN</subject><subject>Hemoglobins</subject><subject>IRRADIATION</subject><subject>Laser beams</subject><subject>Laser power</subject><subject>Lasers</subject><subject>Optical density</subject><subject>RADIATION EFFECTS</subject><subject>Radiation Effects on Tissues</subject><subject>Ruby lasers</subject><subject>SPECTRA</subject><subject>USES</subject><subject>VOLUME</subject><subject>Wavelengths</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1965</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1LwzAYxoMoc05vHhWCZ9vls0kughS_YKDIPIc0S21H14ykCv73Rjqmnt7D8_B7Xn4AnGOUYyTpfG2rnIgc51jhAzDFnKFMYoYOwRQhgjPFCT8GJzGuEUJMMDoBEy65IJxNwXzZOLgw0QVoIjTwxQ-uH1rTwaX3Hax9gKXrOvjqojPBNqfgqDZddGe7OwNv93fL8jFbPD88lbeLzDIihkwZUkjOUZ3GKS-UwI5UilTUMaSkWtWMUmltLZmTrMJMCqGcqmRdrKgtmKQzcDNytx_Vxq1seiqYTm9DuzHhS3vT6v9J3zb63X9qglEhkUiAqxHg49DqaNvB2cb6vnd20IwrgTBNpeuxZIOPMbh6P4CR_pGrk1xNhMY6yU31y79P7cs7mym_GPN1HHz4ZRVJu6D0G_GofIo</recordid><startdate>19651001</startdate><enddate>19651001</enddate><creator>Rounds, Donald E.</creator><creator>Olson, Robert S.</creator><creator>Johnson, Fred M.</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19651001</creationdate><title>The Laser as a Potential Tool for Cell Research</title><author>Rounds, Donald E. ; Olson, Robert S. ; Johnson, Fred M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-9a268550f952356971e2b92b3e40989df4338ccf84e84b148779e9b8f6d3c6483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1965</creationdate><topic>ABSORPTION</topic><topic>BIOLOGY AND MEDICINE</topic><topic>Cell Biology - instrumentation</topic><topic>Cells</topic><topic>CYTOLOGY</topic><topic>DENSITY</topic><topic>DIAGRAMS</topic><topic>Epithelial cells</topic><topic>ERYTHROCYTES</topic><topic>Erythrocytes - radiation effects</topic><topic>HEMOGLOBIN</topic><topic>Hemoglobins</topic><topic>IRRADIATION</topic><topic>Laser beams</topic><topic>Laser power</topic><topic>Lasers</topic><topic>Optical density</topic><topic>RADIATION EFFECTS</topic><topic>Radiation Effects on Tissues</topic><topic>Ruby lasers</topic><topic>SPECTRA</topic><topic>USES</topic><topic>VOLUME</topic><topic>Wavelengths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rounds, Donald E.</creatorcontrib><creatorcontrib>Olson, Robert S.</creatorcontrib><creatorcontrib>Johnson, Fred M.</creatorcontrib><creatorcontrib>Pasadena Foundation for Medical Research and Electro-Optical Systems, Inc., Calif</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Cell Biology (U.S.) Formerly J. Biophys. Biochem. Cytol</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rounds, Donald E.</au><au>Olson, Robert S.</au><au>Johnson, Fred M.</au><aucorp>Pasadena Foundation for Medical Research and Electro-Optical Systems, Inc., Calif</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Laser as a Potential Tool for Cell Research</atitle><jtitle>Journal of Cell Biology (U.S.) Formerly J. Biophys. Biochem. Cytol</jtitle><addtitle>J Cell Biol</addtitle><date>1965-10-01</date><risdate>1965</risdate><volume>27</volume><issue>1</issue><spage>191</spage><epage>197</epage><pages>191-197</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><abstract>Freshly prepared hemoglobin solutions were successively irradiated up to five times with 1 MW (monochromatic wavelength) of green (530 mμ) laser power. Oxygenated hemoglobin showed no detectable change, but the spectral absorption of reduced hemoglobin showed a shift toward the characteristic curve for the oxygenated form. Intact human erythrocytes exposed to a power density of 110 MW/cm2 of green laser radiation showed no appreciable change in diameter or mass, but they became transparent to a wavelength range from 400 to 600 mμ. A similar power density from a ruby laser failed to produce this bleaching effect. This response in the erythrocyte demonstrates a principle which suggests the laser as a tool for cell research: specific molecular components within a cell may be selectively altered by laser irradiation when an appropriate wavelength and a suitable power density are applied.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>5857254</pmid><doi>10.1083/jcb.27.1.191</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9525 |
| ispartof | Journal of Cell Biology (U.S.) Formerly J. Biophys. Biochem. Cytol, 1965-10, Vol.27 (1), p.191-197 |
| issn | 0021-9525 1540-8140 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2106807 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ABSORPTION BIOLOGY AND MEDICINE Cell Biology - instrumentation Cells CYTOLOGY DENSITY DIAGRAMS Epithelial cells ERYTHROCYTES Erythrocytes - radiation effects HEMOGLOBIN Hemoglobins IRRADIATION Laser beams Laser power Lasers Optical density RADIATION EFFECTS Radiation Effects on Tissues Ruby lasers SPECTRA USES VOLUME Wavelengths |
| title | The Laser as a Potential Tool for Cell Research |
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