Pathogenic bacteria and viruses in induced sputum or pharyngeal secretions of adults with stable asthma
Background: Respiratory infections are well known triggers of asthma exacerbations, but their role in stable adult asthma remains unclear. Methods: 103 asthmatics and 30 control subjects were enrolled in the study. Sputum was induced by inhalation of 3% NaCl solution. Oropharyngeal swab specimens we...
Gespeichert in:
| Veröffentlicht in: | Thorax 2006-07, Vol.61 (7), p.579-584 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 584 |
|---|---|
| container_issue | 7 |
| container_start_page | 579 |
| container_title | Thorax |
| container_volume | 61 |
| creator | Harju, T H Leinonen, M Nokso-Koivisto, J Korhonen, T Räty, R He, Q Hovi, T Mertsola, J Bloigu, A Rytilä, P Saikku, P |
| description | Background: Respiratory infections are well known triggers of asthma exacerbations, but their role in stable adult asthma remains unclear. Methods: 103 asthmatics and 30 control subjects were enrolled in the study. Sputum was induced by inhalation of 3% NaCl solution. Oropharyngeal swab specimens were obtained from the posterior wall of the oropharynx. Respiratory specimens were analysed by RT-PCR for rhinovirus, enterovirus and respiratory syncytial virus and by PCR for adenovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae and Bordetella pertussis. Results: Sputum samples from two of the 30 healthy controls (6.7%), five of 53 patients with mild asthma (9.4%), and eight of 50 with moderate asthma (16.0%) were positive for rhinovirus. Rhinovirus positive asthmatic subjects had more asthma symptoms and lower forced expiratory volume in 1 second (FEV1) (79% predicted) than rhinovirus negative cases (93.5% predicted; p = 0.020). Chlamydia pneumoniae PCR was positive in 11 healthy controls (36.6%), 11 mild asthmatics (20.8%), and 11 moderate asthmatics (22%), and PCR positive asthmatics had lower FEV1/FVC than negative cases (78.2% v 80.8%, p = 0.023). Bordetella pertussis PCR was positive in 30 cases: five healthy controls (16.7%), 15 mild asthmatics (28.3%), and 10 moderate asthmatics (20%). Bordetella pertussis positive individuals had lower FEV1/FVC (77.1% v 80.7%, p = 0.012) and more asthma symptoms than B pertussis negative cases. Conclusions: Rhinovirus, C pneumoniae, and B pertussis are found in the sputum or pharyngeal swab specimens of asthmatic subjects without concurrent symptoms of infection or asthma exacerbation, as well as in some healthy controls. Positivity is associated with lower lung function and more frequent asthma symptoms. |
| doi_str_mv | 10.1136/thx.2005.056291 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2104650</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68587669</sourcerecordid><originalsourceid>FETCH-LOGICAL-b553t-96ca61dafbbf2a86eadf6041b4393d116e5703876e3eb4ce26dfba0a28f28003</originalsourceid><addsrcrecordid>eNqFkcuLFDEQxhtR3HH17E0CogehZ5NOp9J9WZDB9_pABvEWqtPV0xn7MSbpdf3v7WGGXfWyEKhD_erLV_UlyWPBl0JIOIvt1TLjXC25gqwUd5KFyKFIZVbC3WTBec5TkBpOkgchbDnnhRD6fnIiQAmttFgkmy8Y23FDg7OsQhvJO2Q41OzS-SlQYG6YXz1ZqlnYTXHq2ejZrkX_e9gQdiyQ9RTdOAQ2NgzrqYuB_XKxZSFi1RHDENseHyb3GuwCPTrW02T9-tV69Ta9-Pzm3erlRVopJWNagkUQNTZV1WRYAGHdAM9FlctS1kIAKc1loYEkVbmlDOqmQo5Z0WQF5_I0OT_I7qaqp9rSED12ZuddPzs2Izrzb2dwrdmMlyYTPAe1F3h-FPDjz4lCNL0LlroOBxqnYKBQ8-9Q3gqKMldcaTmDT_8Dt-Pkh_kIRuhCaBClgpk6O1DWjyF4aq49C272UZs5arOP2hyiniee_L3qDX_MdgaeHQEMFrvG42BduOF0yUEWe4PpgXMh0tV1H_0PA1pqZT59W5n1d_7xw9f1e7Pf_MWBr_rtrS7_AIOg0H0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1781761956</pqid></control><display><type>article</type><title>Pathogenic bacteria and viruses in induced sputum or pharyngeal secretions of adults with stable asthma</title><source>MEDLINE</source><source>BMJ Journals - NESLi2</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Harju, T H ; Leinonen, M ; Nokso-Koivisto, J ; Korhonen, T ; Räty, R ; He, Q ; Hovi, T ; Mertsola, J ; Bloigu, A ; Rytilä, P ; Saikku, P</creator><creatorcontrib>Harju, T H ; Leinonen, M ; Nokso-Koivisto, J ; Korhonen, T ; Räty, R ; He, Q ; Hovi, T ; Mertsola, J ; Bloigu, A ; Rytilä, P ; Saikku, P</creatorcontrib><description>Background: Respiratory infections are well known triggers of asthma exacerbations, but their role in stable adult asthma remains unclear. Methods: 103 asthmatics and 30 control subjects were enrolled in the study. Sputum was induced by inhalation of 3% NaCl solution. Oropharyngeal swab specimens were obtained from the posterior wall of the oropharynx. Respiratory specimens were analysed by RT-PCR for rhinovirus, enterovirus and respiratory syncytial virus and by PCR for adenovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae and Bordetella pertussis. Results: Sputum samples from two of the 30 healthy controls (6.7%), five of 53 patients with mild asthma (9.4%), and eight of 50 with moderate asthma (16.0%) were positive for rhinovirus. Rhinovirus positive asthmatic subjects had more asthma symptoms and lower forced expiratory volume in 1 second (FEV1) (79% predicted) than rhinovirus negative cases (93.5% predicted; p = 0.020). Chlamydia pneumoniae PCR was positive in 11 healthy controls (36.6%), 11 mild asthmatics (20.8%), and 11 moderate asthmatics (22%), and PCR positive asthmatics had lower FEV1/FVC than negative cases (78.2% v 80.8%, p = 0.023). Bordetella pertussis PCR was positive in 30 cases: five healthy controls (16.7%), 15 mild asthmatics (28.3%), and 10 moderate asthmatics (20%). Bordetella pertussis positive individuals had lower FEV1/FVC (77.1% v 80.7%, p = 0.012) and more asthma symptoms than B pertussis negative cases. Conclusions: Rhinovirus, C pneumoniae, and B pertussis are found in the sputum or pharyngeal swab specimens of asthmatic subjects without concurrent symptoms of infection or asthma exacerbation, as well as in some healthy controls. Positivity is associated with lower lung function and more frequent asthma symptoms.</description><identifier>ISSN: 0040-6376</identifier><identifier>EISSN: 1468-3296</identifier><identifier>DOI: 10.1136/thx.2005.056291</identifier><identifier>PMID: 16517571</identifier><identifier>CODEN: THORA7</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and British Thoracic Society</publisher><subject>Adenovirus ; Adult ; Asthma ; Asthma - microbiology ; Asthma - virology ; Asymptomatic ; Bacteria ; Bacterial infections ; Biological and medical sciences ; Bordetella pertussis ; Bordetella pertussis - isolation & purification ; C-reactive protein ; Case-Control Studies ; Chlamydia pneumoniae ; Chlamydophila pneumoniae ; Chlamydophila pneumoniae - isolation & purification ; Chronic obstructive pulmonary disease, asthma ; CRP ; Enterovirus ; Female ; FEV1 ; forced expiratory volume in 1 second ; forced vital capacity ; FVC ; Humans ; Infections ; Lung diseases ; Male ; Medical sciences ; Mycoplasma pneumoniae ; Pathogenesis ; PCR ; Pneumology ; polymerase chain reaction ; Respiratory syncytial virus ; Reverse Transcriptase Polymerase Chain Reaction ; Rhinovirus ; Rhinovirus - isolation & purification ; RSV ; Sputum - microbiology ; Sputum - virology ; Viral infections ; Viruses ; Whooping cough</subject><ispartof>Thorax, 2006-07, Vol.61 (7), p.579-584</ispartof><rights>Copyright 2006 Thorax</rights><rights>2006 INIST-CNRS</rights><rights>Copyright: 2006 Copyright 2006 Thorax</rights><rights>Copyright © 2006 BMJ Publishing Group and British Thoracic Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b553t-96ca61dafbbf2a86eadf6041b4393d116e5703876e3eb4ce26dfba0a28f28003</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://thorax.bmj.com/content/61/7/579.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttps://thorax.bmj.com/content/61/7/579.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,230,314,727,780,784,885,3196,23571,27924,27925,53791,53793,77600,77631</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17906383$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16517571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harju, T H</creatorcontrib><creatorcontrib>Leinonen, M</creatorcontrib><creatorcontrib>Nokso-Koivisto, J</creatorcontrib><creatorcontrib>Korhonen, T</creatorcontrib><creatorcontrib>Räty, R</creatorcontrib><creatorcontrib>He, Q</creatorcontrib><creatorcontrib>Hovi, T</creatorcontrib><creatorcontrib>Mertsola, J</creatorcontrib><creatorcontrib>Bloigu, A</creatorcontrib><creatorcontrib>Rytilä, P</creatorcontrib><creatorcontrib>Saikku, P</creatorcontrib><title>Pathogenic bacteria and viruses in induced sputum or pharyngeal secretions of adults with stable asthma</title><title>Thorax</title><addtitle>Thorax</addtitle><description>Background: Respiratory infections are well known triggers of asthma exacerbations, but their role in stable adult asthma remains unclear. Methods: 103 asthmatics and 30 control subjects were enrolled in the study. Sputum was induced by inhalation of 3% NaCl solution. Oropharyngeal swab specimens were obtained from the posterior wall of the oropharynx. Respiratory specimens were analysed by RT-PCR for rhinovirus, enterovirus and respiratory syncytial virus and by PCR for adenovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae and Bordetella pertussis. Results: Sputum samples from two of the 30 healthy controls (6.7%), five of 53 patients with mild asthma (9.4%), and eight of 50 with moderate asthma (16.0%) were positive for rhinovirus. Rhinovirus positive asthmatic subjects had more asthma symptoms and lower forced expiratory volume in 1 second (FEV1) (79% predicted) than rhinovirus negative cases (93.5% predicted; p = 0.020). Chlamydia pneumoniae PCR was positive in 11 healthy controls (36.6%), 11 mild asthmatics (20.8%), and 11 moderate asthmatics (22%), and PCR positive asthmatics had lower FEV1/FVC than negative cases (78.2% v 80.8%, p = 0.023). Bordetella pertussis PCR was positive in 30 cases: five healthy controls (16.7%), 15 mild asthmatics (28.3%), and 10 moderate asthmatics (20%). Bordetella pertussis positive individuals had lower FEV1/FVC (77.1% v 80.7%, p = 0.012) and more asthma symptoms than B pertussis negative cases. Conclusions: Rhinovirus, C pneumoniae, and B pertussis are found in the sputum or pharyngeal swab specimens of asthmatic subjects without concurrent symptoms of infection or asthma exacerbation, as well as in some healthy controls. Positivity is associated with lower lung function and more frequent asthma symptoms.</description><subject>Adenovirus</subject><subject>Adult</subject><subject>Asthma</subject><subject>Asthma - microbiology</subject><subject>Asthma - virology</subject><subject>Asymptomatic</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Biological and medical sciences</subject><subject>Bordetella pertussis</subject><subject>Bordetella pertussis - isolation & purification</subject><subject>C-reactive protein</subject><subject>Case-Control Studies</subject><subject>Chlamydia pneumoniae</subject><subject>Chlamydophila pneumoniae</subject><subject>Chlamydophila pneumoniae - isolation & purification</subject><subject>Chronic obstructive pulmonary disease, asthma</subject><subject>CRP</subject><subject>Enterovirus</subject><subject>Female</subject><subject>FEV1</subject><subject>forced expiratory volume in 1 second</subject><subject>forced vital capacity</subject><subject>FVC</subject><subject>Humans</subject><subject>Infections</subject><subject>Lung diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mycoplasma pneumoniae</subject><subject>Pathogenesis</subject><subject>PCR</subject><subject>Pneumology</subject><subject>polymerase chain reaction</subject><subject>Respiratory syncytial virus</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rhinovirus</subject><subject>Rhinovirus - isolation & purification</subject><subject>RSV</subject><subject>Sputum - microbiology</subject><subject>Sputum - virology</subject><subject>Viral infections</subject><subject>Viruses</subject><subject>Whooping cough</subject><issn>0040-6376</issn><issn>1468-3296</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkcuLFDEQxhtR3HH17E0CogehZ5NOp9J9WZDB9_pABvEWqtPV0xn7MSbpdf3v7WGGXfWyEKhD_erLV_UlyWPBl0JIOIvt1TLjXC25gqwUd5KFyKFIZVbC3WTBec5TkBpOkgchbDnnhRD6fnIiQAmttFgkmy8Y23FDg7OsQhvJO2Q41OzS-SlQYG6YXz1ZqlnYTXHq2ejZrkX_e9gQdiyQ9RTdOAQ2NgzrqYuB_XKxZSFi1RHDENseHyb3GuwCPTrW02T9-tV69Ta9-Pzm3erlRVopJWNagkUQNTZV1WRYAGHdAM9FlctS1kIAKc1loYEkVbmlDOqmQo5Z0WQF5_I0OT_I7qaqp9rSED12ZuddPzs2Izrzb2dwrdmMlyYTPAe1F3h-FPDjz4lCNL0LlroOBxqnYKBQ8-9Q3gqKMldcaTmDT_8Dt-Pkh_kIRuhCaBClgpk6O1DWjyF4aq49C272UZs5arOP2hyiniee_L3qDX_MdgaeHQEMFrvG42BduOF0yUEWe4PpgXMh0tV1H_0PA1pqZT59W5n1d_7xw9f1e7Pf_MWBr_rtrS7_AIOg0H0</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Harju, T H</creator><creator>Leinonen, M</creator><creator>Nokso-Koivisto, J</creator><creator>Korhonen, T</creator><creator>Räty, R</creator><creator>He, Q</creator><creator>Hovi, T</creator><creator>Mertsola, J</creator><creator>Bloigu, A</creator><creator>Rytilä, P</creator><creator>Saikku, P</creator><general>BMJ Publishing Group Ltd and British Thoracic Society</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><general>BMJ Group</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060701</creationdate><title>Pathogenic bacteria and viruses in induced sputum or pharyngeal secretions of adults with stable asthma</title><author>Harju, T H ; Leinonen, M ; Nokso-Koivisto, J ; Korhonen, T ; Räty, R ; He, Q ; Hovi, T ; Mertsola, J ; Bloigu, A ; Rytilä, P ; Saikku, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b553t-96ca61dafbbf2a86eadf6041b4393d116e5703876e3eb4ce26dfba0a28f28003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adenovirus</topic><topic>Adult</topic><topic>Asthma</topic><topic>Asthma - microbiology</topic><topic>Asthma - virology</topic><topic>Asymptomatic</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Biological and medical sciences</topic><topic>Bordetella pertussis</topic><topic>Bordetella pertussis - isolation & purification</topic><topic>C-reactive protein</topic><topic>Case-Control Studies</topic><topic>Chlamydia pneumoniae</topic><topic>Chlamydophila pneumoniae</topic><topic>Chlamydophila pneumoniae - isolation & purification</topic><topic>Chronic obstructive pulmonary disease, asthma</topic><topic>CRP</topic><topic>Enterovirus</topic><topic>Female</topic><topic>FEV1</topic><topic>forced expiratory volume in 1 second</topic><topic>forced vital capacity</topic><topic>FVC</topic><topic>Humans</topic><topic>Infections</topic><topic>Lung diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mycoplasma pneumoniae</topic><topic>Pathogenesis</topic><topic>PCR</topic><topic>Pneumology</topic><topic>polymerase chain reaction</topic><topic>Respiratory syncytial virus</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rhinovirus</topic><topic>Rhinovirus - isolation & purification</topic><topic>RSV</topic><topic>Sputum - microbiology</topic><topic>Sputum - virology</topic><topic>Viral infections</topic><topic>Viruses</topic><topic>Whooping cough</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harju, T H</creatorcontrib><creatorcontrib>Leinonen, M</creatorcontrib><creatorcontrib>Nokso-Koivisto, J</creatorcontrib><creatorcontrib>Korhonen, T</creatorcontrib><creatorcontrib>Räty, R</creatorcontrib><creatorcontrib>He, Q</creatorcontrib><creatorcontrib>Hovi, T</creatorcontrib><creatorcontrib>Mertsola, J</creatorcontrib><creatorcontrib>Bloigu, A</creatorcontrib><creatorcontrib>Rytilä, P</creatorcontrib><creatorcontrib>Saikku, P</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Thorax</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harju, T H</au><au>Leinonen, M</au><au>Nokso-Koivisto, J</au><au>Korhonen, T</au><au>Räty, R</au><au>He, Q</au><au>Hovi, T</au><au>Mertsola, J</au><au>Bloigu, A</au><au>Rytilä, P</au><au>Saikku, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathogenic bacteria and viruses in induced sputum or pharyngeal secretions of adults with stable asthma</atitle><jtitle>Thorax</jtitle><addtitle>Thorax</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>61</volume><issue>7</issue><spage>579</spage><epage>584</epage><pages>579-584</pages><issn>0040-6376</issn><eissn>1468-3296</eissn><coden>THORA7</coden><abstract>Background: Respiratory infections are well known triggers of asthma exacerbations, but their role in stable adult asthma remains unclear. Methods: 103 asthmatics and 30 control subjects were enrolled in the study. Sputum was induced by inhalation of 3% NaCl solution. Oropharyngeal swab specimens were obtained from the posterior wall of the oropharynx. Respiratory specimens were analysed by RT-PCR for rhinovirus, enterovirus and respiratory syncytial virus and by PCR for adenovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae and Bordetella pertussis. Results: Sputum samples from two of the 30 healthy controls (6.7%), five of 53 patients with mild asthma (9.4%), and eight of 50 with moderate asthma (16.0%) were positive for rhinovirus. Rhinovirus positive asthmatic subjects had more asthma symptoms and lower forced expiratory volume in 1 second (FEV1) (79% predicted) than rhinovirus negative cases (93.5% predicted; p = 0.020). Chlamydia pneumoniae PCR was positive in 11 healthy controls (36.6%), 11 mild asthmatics (20.8%), and 11 moderate asthmatics (22%), and PCR positive asthmatics had lower FEV1/FVC than negative cases (78.2% v 80.8%, p = 0.023). Bordetella pertussis PCR was positive in 30 cases: five healthy controls (16.7%), 15 mild asthmatics (28.3%), and 10 moderate asthmatics (20%). Bordetella pertussis positive individuals had lower FEV1/FVC (77.1% v 80.7%, p = 0.012) and more asthma symptoms than B pertussis negative cases. Conclusions: Rhinovirus, C pneumoniae, and B pertussis are found in the sputum or pharyngeal swab specimens of asthmatic subjects without concurrent symptoms of infection or asthma exacerbation, as well as in some healthy controls. Positivity is associated with lower lung function and more frequent asthma symptoms.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and British Thoracic Society</pub><pmid>16517571</pmid><doi>10.1136/thx.2005.056291</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0040-6376 |
| ispartof | Thorax, 2006-07, Vol.61 (7), p.579-584 |
| issn | 0040-6376 1468-3296 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2104650 |
| source | MEDLINE; BMJ Journals - NESLi2; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adenovirus Adult Asthma Asthma - microbiology Asthma - virology Asymptomatic Bacteria Bacterial infections Biological and medical sciences Bordetella pertussis Bordetella pertussis - isolation & purification C-reactive protein Case-Control Studies Chlamydia pneumoniae Chlamydophila pneumoniae Chlamydophila pneumoniae - isolation & purification Chronic obstructive pulmonary disease, asthma CRP Enterovirus Female FEV1 forced expiratory volume in 1 second forced vital capacity FVC Humans Infections Lung diseases Male Medical sciences Mycoplasma pneumoniae Pathogenesis PCR Pneumology polymerase chain reaction Respiratory syncytial virus Reverse Transcriptase Polymerase Chain Reaction Rhinovirus Rhinovirus - isolation & purification RSV Sputum - microbiology Sputum - virology Viral infections Viruses Whooping cough |
| title | Pathogenic bacteria and viruses in induced sputum or pharyngeal secretions of adults with stable asthma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T04%3A07%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pathogenic%20bacteria%20and%20viruses%20in%20induced%20sputum%20or%20pharyngeal%20secretions%20of%20adults%20with%20stable%20asthma&rft.jtitle=Thorax&rft.au=Harju,%20T%20H&rft.date=2006-07-01&rft.volume=61&rft.issue=7&rft.spage=579&rft.epage=584&rft.pages=579-584&rft.issn=0040-6376&rft.eissn=1468-3296&rft.coden=THORA7&rft_id=info:doi/10.1136/thx.2005.056291&rft_dat=%3Cproquest_pubme%3E68587669%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1781761956&rft_id=info:pmid/16517571&rfr_iscdi=true |