Genistein induces the metastasis suppressor kangai-1 which mediates its anti-invasive effects in TRAMP cancer cells
Previous studies demonstrated a direct correlation with loss of kangai-1 (KAI1), a metastasis suppressor, and poor prognosis in human prostate and other cancers. In this study, we have characterized the age-dependent downregulation of KAI1 in the TRAMP model which was reversed when mice were fed a g...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-09, Vol.361 (1), p.169-175 |
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| creator | El Touny, Lara H. Banerjee, Partha P. |
| description | Previous studies demonstrated a direct correlation with loss of kangai-1 (KAI1), a metastasis suppressor, and poor prognosis in human prostate and other cancers. In this study, we have characterized the age-dependent downregulation of KAI1 in the TRAMP model which was reversed when mice were fed a genistein-enriched diet. We demonstrated here that doses of genistein (5 and 10
μM)—achievable by supplement intake—significantly induced the expression of KAI1, both at the mRNA and protein levels (up to 2.5-fold), and decreased the invasiveness of TRAMP-C2 cells >2.0-fold. We have pinpointed KAI1 as the invasion suppressor, since its knockdown by siRNA restored the invasive potential of genistein-treated TRAMP-C2 cells to control levels. This work provides the first evidence that genistein treatment may counteract KAI1 downregulation, which is observed in many cancer types and therefore, could be used in anti-metastatic therapies. |
| doi_str_mv | 10.1016/j.bbrc.2007.07.010 |
| format | Article |
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μM)—achievable by supplement intake—significantly induced the expression of KAI1, both at the mRNA and protein levels (up to 2.5-fold), and decreased the invasiveness of TRAMP-C2 cells >2.0-fold. We have pinpointed KAI1 as the invasion suppressor, since its knockdown by siRNA restored the invasive potential of genistein-treated TRAMP-C2 cells to control levels. 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μM)—achievable by supplement intake—significantly induced the expression of KAI1, both at the mRNA and protein levels (up to 2.5-fold), and decreased the invasiveness of TRAMP-C2 cells >2.0-fold. We have pinpointed KAI1 as the invasion suppressor, since its knockdown by siRNA restored the invasive potential of genistein-treated TRAMP-C2 cells to control levels. This work provides the first evidence that genistein treatment may counteract KAI1 downregulation, which is observed in many cancer types and therefore, could be used in anti-metastatic therapies.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17658479</pmid><doi>10.1016/j.bbrc.2007.07.010</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2007-09, Vol.361 (1), p.169-175 |
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| subjects | Age Factors Animals Anticarcinogenic Agents - pharmacology CD82 Cell Line, Tumor Disease Progression Genistein Genistein - pharmacology KAI1/kangai Kangai-1 Protein - antagonists & inhibitors Kangai-1 Protein - biosynthesis Kangai-1 Protein - genetics Male Metastasis Mice Neoplasm Invasiveness Phytoestrogen Prostate cancer Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology RNA Interference TRAMP |
| title | Genistein induces the metastasis suppressor kangai-1 which mediates its anti-invasive effects in TRAMP cancer cells |
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