Cep164, a novel centriole appendage protein required for primary cilium formation
Primary cilia (PC) function as microtubule-based sensory antennae projecting from the surface of many eukaryotic cells. They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that functions...
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| Veröffentlicht in: | The Journal of cell biology 2007-10, Vol.179 (2), p.321-330 |
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| container_title | The Journal of cell biology |
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| creator | Graser, Susanne Stierhof, York-Dieter Lavoie, Sébastien B Gassner, Oliver S Lamla, Stefan Le Clech, Mikael Nigg, Erich A |
| description | Primary cilia (PC) function as microtubule-based sensory antennae projecting from the surface of many eukaryotic cells. They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that functions in PC assembly is derived from the mature centriole, a component of the centrosome. Through a small interfering RNA screen we found several centrosomal proteins (Ceps) to be involved in PC formation. One newly identified protein, Cep164, was indispensable for PC formation and hence characterized in detail. By immunogold electron microscopy, Cep164 could be localized to the distal appendages of mature centrioles. In contrast to ninein and Cep170, two components of subdistal appendages, Cep164 persisted at centrioles throughout mitosis. Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures. |
| doi_str_mv | 10.1083/jcb.200707181 |
| format | Article |
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They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that functions in PC assembly is derived from the mature centriole, a component of the centrosome. Through a small interfering RNA screen we found several centrosomal proteins (Ceps) to be involved in PC formation. One newly identified protein, Cep164, was indispensable for PC formation and hence characterized in detail. By immunogold electron microscopy, Cep164 could be localized to the distal appendages of mature centrioles. In contrast to ninein and Cep170, two components of subdistal appendages, Cep164 persisted at centrioles throughout mitosis. Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.200707181</identifier><identifier>PMID: 17954613</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>3T3 cells ; Antibodies ; Antibodies - pharmacology ; Appendages ; Carrier Proteins - metabolism ; Carrier Proteins - ultrastructure ; Cell cycle ; Cell Cycle - drug effects ; Cell Cycle Proteins - metabolism ; Cell Cycle Proteins - ultrastructure ; Cell Line, Tumor ; Cell Nucleus Structures - drug effects ; Cell Nucleus Structures - metabolism ; Centrioles ; Centrioles - drug effects ; Centrioles - metabolism ; Centrosomes ; Cilia ; Cilia - drug effects ; Cilia - metabolism ; Disease ; Electron microscopes ; Eukaryotes ; HeLa cells ; Humans ; Microtubule Proteins ; Protein Transport - drug effects ; Proteins ; Ribonucleic acid ; RNA ; RNA, Small Interfering - metabolism ; Small interfering RNA</subject><ispartof>The Journal of cell biology, 2007-10, Vol.179 (2), p.321-330</ispartof><rights>Copyright 2007 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Oct 22, 2007</rights><rights>Copyright © 2007, The Rockefeller University Press 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-d1373697b89ec4c0343c3228161709e256dc8c14f47dbe3c3b2063c842ba59ec3</citedby><cites>FETCH-LOGICAL-c555t-d1373697b89ec4c0343c3228161709e256dc8c14f47dbe3c3b2063c842ba59ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,313,314,776,780,788,881,27899,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17954613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Graser, Susanne</creatorcontrib><creatorcontrib>Stierhof, York-Dieter</creatorcontrib><creatorcontrib>Lavoie, Sébastien B</creatorcontrib><creatorcontrib>Gassner, Oliver S</creatorcontrib><creatorcontrib>Lamla, Stefan</creatorcontrib><creatorcontrib>Le Clech, Mikael</creatorcontrib><creatorcontrib>Nigg, Erich A</creatorcontrib><title>Cep164, a novel centriole appendage protein required for primary cilium formation</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Primary cilia (PC) function as microtubule-based sensory antennae projecting from the surface of many eukaryotic cells. They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that functions in PC assembly is derived from the mature centriole, a component of the centrosome. Through a small interfering RNA screen we found several centrosomal proteins (Ceps) to be involved in PC formation. One newly identified protein, Cep164, was indispensable for PC formation and hence characterized in detail. By immunogold electron microscopy, Cep164 could be localized to the distal appendages of mature centrioles. In contrast to ninein and Cep170, two components of subdistal appendages, Cep164 persisted at centrioles throughout mitosis. Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures.</description><subject>3T3 cells</subject><subject>Antibodies</subject><subject>Antibodies - pharmacology</subject><subject>Appendages</subject><subject>Carrier Proteins - metabolism</subject><subject>Carrier Proteins - ultrastructure</subject><subject>Cell cycle</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell Cycle Proteins - ultrastructure</subject><subject>Cell Line, Tumor</subject><subject>Cell Nucleus Structures - drug effects</subject><subject>Cell Nucleus Structures - metabolism</subject><subject>Centrioles</subject><subject>Centrioles - drug effects</subject><subject>Centrioles - metabolism</subject><subject>Centrosomes</subject><subject>Cilia</subject><subject>Cilia - drug effects</subject><subject>Cilia - metabolism</subject><subject>Disease</subject><subject>Electron microscopes</subject><subject>Eukaryotes</subject><subject>HeLa cells</subject><subject>Humans</subject><subject>Microtubule Proteins</subject><subject>Protein Transport - drug effects</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Small interfering RNA</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS1ERYfCkiUQseiKlHv9iJ1NJTSCUqlShaBry3GcwaMkTu2kEv8eDzMaHpuuLN3z-dxjH0JeIVwgKPZha5sLCiBBosInZIWCQ6mQw1OyAqBY1oKKU_I8pS0AcMnZM3KKsha8QrYiX9duwoq_L0wxhgfXF9aNc_Shd4WZJje2ZuOKKYbZ-bGI7n7x0bVFF2Ie-sHEn4X1vV-G3Wgwsw_jC3LSmT65l4fzjNx9_vR9_aW8ub26Xn-8Ka0QYi5bZJJVtWxU7Sy3wDizjFKFFUqoHRVVa5VF3nHZNi5rDYWKWcVpY0S-ws7I5d53WprBtb9zm14fYulgvP5XGf0PvQkPOvtwWclscH4wiOF-cWnWg0_W9b0ZXViSrvIuTkX9KEiBSaSVyuC7_8BtWOKYf0FTlJjbwJ1buYdsDClF1x0jI-hdpTpXqo-VZv7N3-_8Qx86zMDrPbBNc4hHnQGI3DfN-tu93pmgzSb6pO--UcAMKLrbwn4Bozmujg</recordid><startdate>20071022</startdate><enddate>20071022</enddate><creator>Graser, Susanne</creator><creator>Stierhof, York-Dieter</creator><creator>Lavoie, Sébastien B</creator><creator>Gassner, Oliver S</creator><creator>Lamla, Stefan</creator><creator>Le Clech, Mikael</creator><creator>Nigg, Erich A</creator><general>The Rockefeller University Press</general><general>Rockefeller University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20071022</creationdate><title>Cep164, a novel centriole appendage protein required for primary cilium formation</title><author>Graser, Susanne ; 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They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that functions in PC assembly is derived from the mature centriole, a component of the centrosome. Through a small interfering RNA screen we found several centrosomal proteins (Ceps) to be involved in PC formation. One newly identified protein, Cep164, was indispensable for PC formation and hence characterized in detail. By immunogold electron microscopy, Cep164 could be localized to the distal appendages of mature centrioles. In contrast to ninein and Cep170, two components of subdistal appendages, Cep164 persisted at centrioles throughout mitosis. Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>17954613</pmid><doi>10.1083/jcb.200707181</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3T3 cells Antibodies Antibodies - pharmacology Appendages Carrier Proteins - metabolism Carrier Proteins - ultrastructure Cell cycle Cell Cycle - drug effects Cell Cycle Proteins - metabolism Cell Cycle Proteins - ultrastructure Cell Line, Tumor Cell Nucleus Structures - drug effects Cell Nucleus Structures - metabolism Centrioles Centrioles - drug effects Centrioles - metabolism Centrosomes Cilia Cilia - drug effects Cilia - metabolism Disease Electron microscopes Eukaryotes HeLa cells Humans Microtubule Proteins Protein Transport - drug effects Proteins Ribonucleic acid RNA RNA, Small Interfering - metabolism Small interfering RNA |
| title | Cep164, a novel centriole appendage protein required for primary cilium formation |
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