CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1
Melanocytes reside within the basal layer of the human epidermis, where they attach to the basement membrane and replicate at a rate proportionate to that of keratinocytes, maintaining a lifelong stable ratio. In this study, we report that coculturing melanocytes with keratinocytes up-regulated CCN3...
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| Veröffentlicht in: | The Journal of cell biology 2006-11, Vol.175 (4), p.563-569 |
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| container_title | The Journal of cell biology |
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| creator | Fukunaga-Kalabis, Mizuho Martinez, Gabriela Liu, Zhao-Jun Kalabis, Jiri Mrass, Paul Weninger, Wolfgang Firth, Sue M Planque, Nathalie Perbal, Bernard Herlyn, Meenhard |
| description | Melanocytes reside within the basal layer of the human epidermis, where they attach to the basement membrane and replicate at a rate proportionate to that of keratinocytes, maintaining a lifelong stable ratio. In this study, we report that coculturing melanocytes with keratinocytes up-regulated CCN3, a matricellular protein that we subsequently found to be critical for the spatial localization of melanocytes to the basement membrane. CCN3 knockdown cells were dissociated either upward to the suprabasal layers of the epidermis or downward into the dermis. The overexpression of CCN3 increased adhesion to collagen type IV, the major component of the basement membrane. As the receptor responsible for CCN3-mediated melanocyte localization, we identified discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase that acts as a collagen IV adhesion receptor. DDR1 knockdown decreased melanocyte adhesion to collagen IV and shifted melanocyte localization in a manner similar to CCN3 knockdown. These results demonstrate an intricate and necessary communication between keratinocytes and melanocytes in maintaining normal epidermal homeostasis. |
| doi_str_mv | 10.1083/jcb.200602132 |
| format | Article |
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In this study, we report that coculturing melanocytes with keratinocytes up-regulated CCN3, a matricellular protein that we subsequently found to be critical for the spatial localization of melanocytes to the basement membrane. CCN3 knockdown cells were dissociated either upward to the suprabasal layers of the epidermis or downward into the dermis. The overexpression of CCN3 increased adhesion to collagen type IV, the major component of the basement membrane. As the receptor responsible for CCN3-mediated melanocyte localization, we identified discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase that acts as a collagen IV adhesion receptor. DDR1 knockdown decreased melanocyte adhesion to collagen IV and shifted melanocyte localization in a manner similar to CCN3 knockdown. These results demonstrate an intricate and necessary communication between keratinocytes and melanocytes in maintaining normal epidermal homeostasis.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.200602132</identifier><identifier>PMID: 17101694</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Atoms & subatomic particles ; Basement membrane ; Basement Membrane - cytology ; Cell Adhesion ; Cell growth ; Cell lines ; Cell Movement ; Coculture techniques ; Collagens ; Connective Tissue Growth Factor ; Discoidin Domain Receptor 1 ; Gene Expression ; Gene Expression Regulation ; Humans ; Immediate-Early Proteins - deficiency ; Immediate-Early Proteins - genetics ; Immediate-Early Proteins - metabolism ; Intercellular Signaling Peptides and Proteins - deficiency ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - metabolism ; Keratinocytes ; Keratinocytes - cytology ; Melanocytes ; Melanocytes - cytology ; Membranes ; Nephroblastoma Overexpressed Protein ; Receptor Protein-Tyrosine Kinases - metabolism ; Receptors ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Skin ; Skin - cytology ; Small interfering RNA ; Stem cells ; Studies</subject><ispartof>The Journal of cell biology, 2006-11, Vol.175 (4), p.563-569</ispartof><rights>Copyright 2006 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Nov 20, 2006</rights><rights>Copyright © 2006, The Rockefeller University Press 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-d8a2d8d077c36ed5849c960432f9aeb9a8a9b02d21aa2eb3a4fb23996108fbb53</citedby><cites>FETCH-LOGICAL-c554t-d8a2d8d077c36ed5849c960432f9aeb9a8a9b02d21aa2eb3a4fb23996108fbb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17101694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukunaga-Kalabis, Mizuho</creatorcontrib><creatorcontrib>Martinez, Gabriela</creatorcontrib><creatorcontrib>Liu, Zhao-Jun</creatorcontrib><creatorcontrib>Kalabis, Jiri</creatorcontrib><creatorcontrib>Mrass, Paul</creatorcontrib><creatorcontrib>Weninger, Wolfgang</creatorcontrib><creatorcontrib>Firth, Sue M</creatorcontrib><creatorcontrib>Planque, Nathalie</creatorcontrib><creatorcontrib>Perbal, Bernard</creatorcontrib><creatorcontrib>Herlyn, Meenhard</creatorcontrib><title>CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Melanocytes reside within the basal layer of the human epidermis, where they attach to the basement membrane and replicate at a rate proportionate to that of keratinocytes, maintaining a lifelong stable ratio. 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These results demonstrate an intricate and necessary communication between keratinocytes and melanocytes in maintaining normal epidermal homeostasis.</description><subject>Atoms & subatomic particles</subject><subject>Basement membrane</subject><subject>Basement Membrane - cytology</subject><subject>Cell Adhesion</subject><subject>Cell growth</subject><subject>Cell lines</subject><subject>Cell Movement</subject><subject>Coculture techniques</subject><subject>Collagens</subject><subject>Connective Tissue Growth Factor</subject><subject>Discoidin Domain Receptor 1</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Immediate-Early Proteins - deficiency</subject><subject>Immediate-Early Proteins - genetics</subject><subject>Immediate-Early Proteins - metabolism</subject><subject>Intercellular Signaling Peptides and Proteins - deficiency</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Keratinocytes</subject><subject>Keratinocytes - cytology</subject><subject>Melanocytes</subject><subject>Melanocytes - cytology</subject><subject>Membranes</subject><subject>Nephroblastoma Overexpressed Protein</subject><subject>Receptor Protein-Tyrosine Kinases - metabolism</subject><subject>Receptors</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Skin</subject><subject>Skin - cytology</subject><subject>Small interfering RNA</subject><subject>Stem cells</subject><subject>Studies</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuP0zAQxi0EYkvhyA2BxYFblvEz8QUJtbykFUgse7YmjtOmJHGxE6Tlr8elVXlcOFnj76dv5psh5DGDSwaVeLlz9SUH0MCZ4HfIgikJRcUk3CULyJ-FUVxdkAcp7QBAllLcJxesZMC0kQtyvVp9FNSFcYqhT1Ssadrj1GFP--Cw737kIow0tHTwPY7B3U4-0W6k09bT7TzgSNPXX2UM82ZL1-vP7CG512Kf_KPTuyQ3b998Wb0vrj69-7B6fVU4peRUNBXypmqgLJ3QvlGVNM5okIK3Bn1tsEJTA284Q-S-FijbmgtjdM7d1rUSS_Lq6Luf68E3zucQ2Nt97AaMtzZgZ_9Wxm5rN-G75aClMiIbvDgZxPBt9mmyQ5ec73NQH-ZkdcVKLoT8L8iMNKUuDyM9_wfchTmOeQuWH5Yuy9x3SYoj5GJIKfr2PDIDeziqzUe156Nm_umfOX_Tpytm4MkR2KUpxLMumcpNdZafHeUWg8VN7JK9uebABDCWPXglfgJBJK8N</recordid><startdate>20061120</startdate><enddate>20061120</enddate><creator>Fukunaga-Kalabis, Mizuho</creator><creator>Martinez, Gabriela</creator><creator>Liu, Zhao-Jun</creator><creator>Kalabis, Jiri</creator><creator>Mrass, Paul</creator><creator>Weninger, Wolfgang</creator><creator>Firth, Sue M</creator><creator>Planque, Nathalie</creator><creator>Perbal, Bernard</creator><creator>Herlyn, Meenhard</creator><general>The Rockefeller University Press</general><general>Rockefeller University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20061120</creationdate><title>CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1</title><author>Fukunaga-Kalabis, Mizuho ; 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In this study, we report that coculturing melanocytes with keratinocytes up-regulated CCN3, a matricellular protein that we subsequently found to be critical for the spatial localization of melanocytes to the basement membrane. CCN3 knockdown cells were dissociated either upward to the suprabasal layers of the epidermis or downward into the dermis. The overexpression of CCN3 increased adhesion to collagen type IV, the major component of the basement membrane. As the receptor responsible for CCN3-mediated melanocyte localization, we identified discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase that acts as a collagen IV adhesion receptor. DDR1 knockdown decreased melanocyte adhesion to collagen IV and shifted melanocyte localization in a manner similar to CCN3 knockdown. These results demonstrate an intricate and necessary communication between keratinocytes and melanocytes in maintaining normal epidermal homeostasis.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>17101694</pmid><doi>10.1083/jcb.200602132</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Atoms & subatomic particles Basement membrane Basement Membrane - cytology Cell Adhesion Cell growth Cell lines Cell Movement Coculture techniques Collagens Connective Tissue Growth Factor Discoidin Domain Receptor 1 Gene Expression Gene Expression Regulation Humans Immediate-Early Proteins - deficiency Immediate-Early Proteins - genetics Immediate-Early Proteins - metabolism Intercellular Signaling Peptides and Proteins - deficiency Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Keratinocytes Keratinocytes - cytology Melanocytes Melanocytes - cytology Membranes Nephroblastoma Overexpressed Protein Receptor Protein-Tyrosine Kinases - metabolism Receptors RNA, Messenger - genetics RNA, Messenger - metabolism Skin Skin - cytology Small interfering RNA Stem cells Studies |
| title | CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1 |
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