Molecular cloning, chromosomal mapping, and sequence analysis of copper resistance genes from Xanthomonas campestris pv. juglandis: homology with small blue copper proteins and multicopper oxidase
Copper-resistant strains of Xanthomonas campestris pv. juglandis occur in walnut orchards throughout northern California. The copper resistance genes from a copper-resistant strain C5 of X. campestris pv. juglandis were cloned and located on a 4.9-kb ClaI fragment, which hybridized only to DNA of co...
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| Veröffentlicht in: | Journal of Bacteriology 1994, Vol.176 (1), p.173-188 |
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| description | Copper-resistant strains of Xanthomonas campestris pv. juglandis occur in walnut orchards throughout northern California. The copper resistance genes from a copper-resistant strain C5 of X. campestris pv. juglandis were cloned and located on a 4.9-kb ClaI fragment, which hybridized only to DNA of copper-resistant strains of X. campestris pv. juglandis, and was part of an approximately 20-kb region which was conserved among such strains of X. campestris pv. juglandis. Hybridization analysis indicated that the copper resistance genes were located on the chromosome. Plasmids conferring copper resistance were not detected in copper-resistant strains, nor did mating with copper-sensitive strains result in copper-resistant transconjugants. Copper resistance genes from X. campestris pv. juglandis shared nucleotide sequence similarity with copper resistance genes from Pseudomonas syringae pv. tomato, P. syringae, and X. campestris pv. vesicatoria. DNA sequence analysis of the 4.9-kb fragment from strain C5 revealed that the sequence had an overall G+C content of 58.7%, and four open reading frames (ORF1 to ORF4), oriented in the same direction. All four ORFs were required for full expression of copper resistance, on the basis of Tn3-spice insertional inactivation and deletion analysis. The predicted amino acid sequences of ORF1 to ORF4 showed 65, 45, 47, and 40% identity with CopA, CopB, CopC, and CopD, respectively, from P. syringae pv. tomato. The most conserved regions are ORF1 and CopA and the C-terminal region (166 amino acids from the C terminus) of ORF2 and CopB. The hydrophobicity profiles of each pair of predicted polypeptides are similar except for the N terminus of ORF2 and CopB. Four histidine-rich polypeptide regions in ORF1 and CopA strongly resembled the copper-binding motifs of small blue copper proteins and multicopper oxidases, such as fungal laccases, plant ascorbate oxidase, and human ceruloplasmin. Putative copper li. |
| doi_str_mv | 10.1128/jb.176.1.173-188.1994 |
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J ; SCHROTH, M. N</creator><creatorcontrib>YUANG-AN LEE ; HENDSON, M ; PANOPOULOS, N. J ; SCHROTH, M. N</creatorcontrib><description>Copper-resistant strains of Xanthomonas campestris pv. juglandis occur in walnut orchards throughout northern California. The copper resistance genes from a copper-resistant strain C5 of X. campestris pv. juglandis were cloned and located on a 4.9-kb ClaI fragment, which hybridized only to DNA of copper-resistant strains of X. campestris pv. juglandis, and was part of an approximately 20-kb region which was conserved among such strains of X. campestris pv. juglandis. Hybridization analysis indicated that the copper resistance genes were located on the chromosome. Plasmids conferring copper resistance were not detected in copper-resistant strains, nor did mating with copper-sensitive strains result in copper-resistant transconjugants. Copper resistance genes from X. campestris pv. juglandis shared nucleotide sequence similarity with copper resistance genes from Pseudomonas syringae pv. tomato, P. syringae, and X. campestris pv. vesicatoria. DNA sequence analysis of the 4.9-kb fragment from strain C5 revealed that the sequence had an overall G+C content of 58.7%, and four open reading frames (ORF1 to ORF4), oriented in the same direction. All four ORFs were required for full expression of copper resistance, on the basis of Tn3-spice insertional inactivation and deletion analysis. The predicted amino acid sequences of ORF1 to ORF4 showed 65, 45, 47, and 40% identity with CopA, CopB, CopC, and CopD, respectively, from P. syringae pv. tomato. The most conserved regions are ORF1 and CopA and the C-terminal region (166 amino acids from the C terminus) of ORF2 and CopB. The hydrophobicity profiles of each pair of predicted polypeptides are similar except for the N terminus of ORF2 and CopB. Four histidine-rich polypeptide regions in ORF1 and CopA strongly resembled the copper-binding motifs of small blue copper proteins and multicopper oxidases, such as fungal laccases, plant ascorbate oxidase, and human ceruloplasmin. Putative copper li.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/jb.176.1.173-188.1994</identifier><identifier>PMID: 8282694</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; bacteria ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; Biochemistry ; Biological and medical sciences ; chromosome ; Chromosome Mapping ; chromosomes ; Chromosomes, Bacterial ; Cloning, Molecular ; cobre ; Conserved Sequence ; Copper ; Copper - pharmacology ; cromosomas ; cuivre ; DNA Mutational Analysis ; Drug Resistance, Microbial - genetics ; Fundamental and applied biological sciences. Psychology ; gene ; Genes ; Genes, Bacterial - genetics ; genetica ; Genetics ; genetique ; Metalloproteins - genetics ; Microbiology ; Molecular Sequence Data ; Nucleic Acid Hybridization ; nucleotide sequence ; oxidoreductases ; Oxidoreductases - genetics ; oxidorreductasas ; oxydoreductase ; proteinas ; proteine ; proteins ; pseudomonas syringae ; resistance aux facteurs nuisibles ; resistance to injurious factors ; resistencia a agentes daninos ; secuencia nucleica ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; sequence nucleique ; xanthomonas campestris ; Xanthomonas campestris - genetics</subject><ispartof>Journal of Bacteriology, 1994, Vol.176 (1), p.173-188</ispartof><rights>1994 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Jan 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-fa0c8a4e79f103dc432ed9c7462ec10f2c89ec0e73f0a8b966845d75825a57293</citedby><cites>FETCH-LOGICAL-c592t-fa0c8a4e79f103dc432ed9c7462ec10f2c89ec0e73f0a8b966845d75825a57293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC205029/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC205029/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3960111$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8282694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YUANG-AN LEE</creatorcontrib><creatorcontrib>HENDSON, M</creatorcontrib><creatorcontrib>PANOPOULOS, N. J</creatorcontrib><creatorcontrib>SCHROTH, M. N</creatorcontrib><title>Molecular cloning, chromosomal mapping, and sequence analysis of copper resistance genes from Xanthomonas campestris pv. juglandis: homology with small blue copper proteins and multicopper oxidase</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>Copper-resistant strains of Xanthomonas campestris pv. juglandis occur in walnut orchards throughout northern California. The copper resistance genes from a copper-resistant strain C5 of X. campestris pv. juglandis were cloned and located on a 4.9-kb ClaI fragment, which hybridized only to DNA of copper-resistant strains of X. campestris pv. juglandis, and was part of an approximately 20-kb region which was conserved among such strains of X. campestris pv. juglandis. Hybridization analysis indicated that the copper resistance genes were located on the chromosome. Plasmids conferring copper resistance were not detected in copper-resistant strains, nor did mating with copper-sensitive strains result in copper-resistant transconjugants. Copper resistance genes from X. campestris pv. juglandis shared nucleotide sequence similarity with copper resistance genes from Pseudomonas syringae pv. tomato, P. syringae, and X. campestris pv. vesicatoria. DNA sequence analysis of the 4.9-kb fragment from strain C5 revealed that the sequence had an overall G+C content of 58.7%, and four open reading frames (ORF1 to ORF4), oriented in the same direction. All four ORFs were required for full expression of copper resistance, on the basis of Tn3-spice insertional inactivation and deletion analysis. The predicted amino acid sequences of ORF1 to ORF4 showed 65, 45, 47, and 40% identity with CopA, CopB, CopC, and CopD, respectively, from P. syringae pv. tomato. The most conserved regions are ORF1 and CopA and the C-terminal region (166 amino acids from the C terminus) of ORF2 and CopB. The hydrophobicity profiles of each pair of predicted polypeptides are similar except for the N terminus of ORF2 and CopB. Four histidine-rich polypeptide regions in ORF1 and CopA strongly resembled the copper-binding motifs of small blue copper proteins and multicopper oxidases, such as fungal laccases, plant ascorbate oxidase, and human ceruloplasmin. Putative copper li.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>chromosome</subject><subject>Chromosome Mapping</subject><subject>chromosomes</subject><subject>Chromosomes, Bacterial</subject><subject>Cloning, Molecular</subject><subject>cobre</subject><subject>Conserved Sequence</subject><subject>Copper</subject><subject>Copper - pharmacology</subject><subject>cromosomas</subject><subject>cuivre</subject><subject>DNA Mutational Analysis</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>genetica</subject><subject>Genetics</subject><subject>genetique</subject><subject>Metalloproteins - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>nucleotide sequence</subject><subject>oxidoreductases</subject><subject>Oxidoreductases - genetics</subject><subject>oxidorreductasas</subject><subject>oxydoreductase</subject><subject>proteinas</subject><subject>proteine</subject><subject>proteins</subject><subject>pseudomonas syringae</subject><subject>resistance aux facteurs nuisibles</subject><subject>resistance to injurious factors</subject><subject>resistencia a agentes daninos</subject><subject>secuencia nucleica</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>sequence nucleique</subject><subject>xanthomonas campestris</subject><subject>Xanthomonas campestris - genetics</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl2P1CAUhhujWcfVn7CGGOOVHfkoLZh4YTZ-JWu80E28Iww9bZnQUqHddf6fP0w6M05cb7yBwPuclwO8WXZB8JoQKl5tN2tSlWuSRpYTIdZEyuJetiJYipxzhu9nK4wpySWR7GH2KMYtxqQoOD3LzgQVtJTFKvv12Tsws9MBGecHO7QvkemC7330vXao1-O439RDjSL8mGEwkBba7aKNyDfI-HGEgAKk9aQXtYUBImqSCfquh6lLZoOOyOh-hDiFVDberNF2bl0ytfE1Wgjn2x26tVOHYjrXoY2b4Y_3GPwEdoj7JvrZTfYo-J-21hEeZw8a7SI8Oc7n2fX7d98uP-ZXXz58unx7lRsu6ZQ3GhuhC6hkQzCrTcEo1NJURUnBENxQIyQYDBVrsBYbWZai4HXFBeWaV1Sy8-zNwXecNz3UBoYpaKfGYHsddsprq-4qg-1U628UxRzv618c64NPLxkn1dtowKV3AD9HVZWMikrg_4KkFIRzvIDP_gG3fg7pd6KitMJcsqJIED9AJvgYAzSnjglWS5bUdqNSlhRJI1MpS2rJUqq7-Pu6p6pjeJL-_KjraLRrQvp9G08YkyUmhCQMHbDOtt2tDaB07O8cmZCnB6TRXuk2RURdf009pCtSThlmvwG3OexQ</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>YUANG-AN LEE</creator><creator>HENDSON, M</creator><creator>PANOPOULOS, N. J</creator><creator>SCHROTH, M. N</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1994</creationdate><title>Molecular cloning, chromosomal mapping, and sequence analysis of copper resistance genes from Xanthomonas campestris pv. juglandis: homology with small blue copper proteins and multicopper oxidase</title><author>YUANG-AN LEE ; HENDSON, M ; PANOPOULOS, N. J ; SCHROTH, M. 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Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>chromosome</topic><topic>Chromosome Mapping</topic><topic>chromosomes</topic><topic>Chromosomes, Bacterial</topic><topic>Cloning, Molecular</topic><topic>cobre</topic><topic>Conserved Sequence</topic><topic>Copper</topic><topic>Copper - pharmacology</topic><topic>cromosomas</topic><topic>cuivre</topic><topic>DNA Mutational Analysis</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>genetica</topic><topic>Genetics</topic><topic>genetique</topic><topic>Metalloproteins - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>nucleotide sequence</topic><topic>oxidoreductases</topic><topic>Oxidoreductases - genetics</topic><topic>oxidorreductasas</topic><topic>oxydoreductase</topic><topic>proteinas</topic><topic>proteine</topic><topic>proteins</topic><topic>pseudomonas syringae</topic><topic>resistance aux facteurs nuisibles</topic><topic>resistance to injurious factors</topic><topic>resistencia a agentes daninos</topic><topic>secuencia nucleica</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>sequence nucleique</topic><topic>xanthomonas campestris</topic><topic>Xanthomonas campestris - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YUANG-AN LEE</creatorcontrib><creatorcontrib>HENDSON, M</creatorcontrib><creatorcontrib>PANOPOULOS, N. 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N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YUANG-AN LEE</au><au>HENDSON, M</au><au>PANOPOULOS, N. J</au><au>SCHROTH, M. N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning, chromosomal mapping, and sequence analysis of copper resistance genes from Xanthomonas campestris pv. juglandis: homology with small blue copper proteins and multicopper oxidase</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>1994</date><risdate>1994</risdate><volume>176</volume><issue>1</issue><spage>173</spage><epage>188</epage><pages>173-188</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>Copper-resistant strains of Xanthomonas campestris pv. juglandis occur in walnut orchards throughout northern California. The copper resistance genes from a copper-resistant strain C5 of X. campestris pv. juglandis were cloned and located on a 4.9-kb ClaI fragment, which hybridized only to DNA of copper-resistant strains of X. campestris pv. juglandis, and was part of an approximately 20-kb region which was conserved among such strains of X. campestris pv. juglandis. Hybridization analysis indicated that the copper resistance genes were located on the chromosome. Plasmids conferring copper resistance were not detected in copper-resistant strains, nor did mating with copper-sensitive strains result in copper-resistant transconjugants. Copper resistance genes from X. campestris pv. juglandis shared nucleotide sequence similarity with copper resistance genes from Pseudomonas syringae pv. tomato, P. syringae, and X. campestris pv. vesicatoria. DNA sequence analysis of the 4.9-kb fragment from strain C5 revealed that the sequence had an overall G+C content of 58.7%, and four open reading frames (ORF1 to ORF4), oriented in the same direction. All four ORFs were required for full expression of copper resistance, on the basis of Tn3-spice insertional inactivation and deletion analysis. The predicted amino acid sequences of ORF1 to ORF4 showed 65, 45, 47, and 40% identity with CopA, CopB, CopC, and CopD, respectively, from P. syringae pv. tomato. The most conserved regions are ORF1 and CopA and the C-terminal region (166 amino acids from the C terminus) of ORF2 and CopB. The hydrophobicity profiles of each pair of predicted polypeptides are similar except for the N terminus of ORF2 and CopB. Four histidine-rich polypeptide regions in ORF1 and CopA strongly resembled the copper-binding motifs of small blue copper proteins and multicopper oxidases, such as fungal laccases, plant ascorbate oxidase, and human ceruloplasmin. Putative copper li.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8282694</pmid><doi>10.1128/jb.176.1.173-188.1994</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Bacteriology, 1994, Vol.176 (1), p.173-188 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence bacteria Bacterial Proteins - genetics Bacteriology Base Sequence Biochemistry Biological and medical sciences chromosome Chromosome Mapping chromosomes Chromosomes, Bacterial Cloning, Molecular cobre Conserved Sequence Copper Copper - pharmacology cromosomas cuivre DNA Mutational Analysis Drug Resistance, Microbial - genetics Fundamental and applied biological sciences. Psychology gene Genes Genes, Bacterial - genetics genetica Genetics genetique Metalloproteins - genetics Microbiology Molecular Sequence Data Nucleic Acid Hybridization nucleotide sequence oxidoreductases Oxidoreductases - genetics oxidorreductasas oxydoreductase proteinas proteine proteins pseudomonas syringae resistance aux facteurs nuisibles resistance to injurious factors resistencia a agentes daninos secuencia nucleica Sequence Analysis, DNA Sequence Homology, Amino Acid sequence nucleique xanthomonas campestris Xanthomonas campestris - genetics |
| title | Molecular cloning, chromosomal mapping, and sequence analysis of copper resistance genes from Xanthomonas campestris pv. juglandis: homology with small blue copper proteins and multicopper oxidase |
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