The multidrug transporters belonging to major facilitator superfamily in Mycobacterium tuberculosis
Both intrinsic and acquired multidrug resistance play an important role in the insurgence of tuberculosis. Detailed knowledge of the molecular basis of drug recognition and transport by multidrug transport systems is required for the development of new antibiotics that are not extruded or of inhibit...
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| Veröffentlicht in: | Molecular medicine (Cambridge, Mass.) Mass.), 2002-11, Vol.8 (11), p.714-724 |
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| creator | De Rossi, Edda Arrigo, Patrizio Bellinzoni, Marco Silva, Pedro A E Martín, Carlos Aínsa, José A Guglierame, Paola Riccardi, Giovanna |
| description | Both intrinsic and acquired multidrug resistance play an important role in the insurgence of tuberculosis. Detailed knowledge of the molecular basis of drug recognition and transport by multidrug transport systems is required for the development of new antibiotics that are not extruded or of inhibitors that block the multidrug transporter and allow traditional antibiotics to be effective.
We have undertaken the inventory of the drug transporters subfamily, included in the major facilitator superfamily (MFS), encoded by the complete genome of Mycobacterium tuberculosis (MTB). These proteins were identified on the basis of their characteristic stretches of amino acids and transmembrane segments (TMS) number.
Genome analysis and searches of homology between the identified transporters and proteins characterized in other organisms revealed 16 open reading frames encoding putative drug efflux pumps belonging to MFS. In the case of two of them, we also have demonstrated that they function as drug efflux proteins. |
| doi_str_mv | 10.1007/bf03402035 |
| format | Article |
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We have undertaken the inventory of the drug transporters subfamily, included in the major facilitator superfamily (MFS), encoded by the complete genome of Mycobacterium tuberculosis (MTB). These proteins were identified on the basis of their characteristic stretches of amino acids and transmembrane segments (TMS) number.
Genome analysis and searches of homology between the identified transporters and proteins characterized in other organisms revealed 16 open reading frames encoding putative drug efflux pumps belonging to MFS. In the case of two of them, we also have demonstrated that they function as drug efflux proteins.</description><identifier>ISSN: 1076-1551</identifier><identifier>EISSN: 1528-3658</identifier><identifier>DOI: 10.1007/bf03402035</identifier><identifier>PMID: 12520088</identifier><language>eng</language><publisher>England: Feinstein Institute for Medical Research</publisher><subject>Amino Acid Sequence ; Anti-Infective Agents ; Anti-Infective Agents - pharmacology ; Biochemistry, Molecular Biology ; Bioinformatics ; Biological Physics ; Biological Transport, Active ; Cellular Biology ; Chemical Sciences ; Cloning, Molecular ; Computational Biology ; Computer Science ; Conserved Sequence ; Cristallography ; Drug Resistance, Bacterial ; Drug Resistance, Bacterial - physiology ; Drug Resistance, Multiple ; Evolution, Molecular ; Gene Expression Regulation ; Genome, Bacterial ; Life Sciences ; Molecular Sequence Data ; Multidrug Resistance-Associated Proteins ; Multidrug Resistance-Associated Proteins - metabolism ; Multigene Family ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - genetics ; Norfloxacin ; Norfloxacin - pharmacology ; Phylogeny ; Physics ; Sequence Homology, Amino Acid ; Structural Biology</subject><ispartof>Molecular medicine (Cambridge, Mass.), 2002-11, Vol.8 (11), p.714-724</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-4a92c62688961bc6b103cbcded31b13302d9db2ebc8c3a453a16bb5749bc57f63</citedby><cites>FETCH-LOGICAL-c478t-4a92c62688961bc6b103cbcded31b13302d9db2ebc8c3a453a16bb5749bc57f63</cites><orcidid>0000-0002-8887-6917 ; 0000-0002-2661-169X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2039957/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2039957/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12520088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://pasteur.hal.science/pasteur-03257806$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>De Rossi, Edda</creatorcontrib><creatorcontrib>Arrigo, Patrizio</creatorcontrib><creatorcontrib>Bellinzoni, Marco</creatorcontrib><creatorcontrib>Silva, Pedro A E</creatorcontrib><creatorcontrib>Martín, Carlos</creatorcontrib><creatorcontrib>Aínsa, José A</creatorcontrib><creatorcontrib>Guglierame, Paola</creatorcontrib><creatorcontrib>Riccardi, Giovanna</creatorcontrib><title>The multidrug transporters belonging to major facilitator superfamily in Mycobacterium tuberculosis</title><title>Molecular medicine (Cambridge, Mass.)</title><addtitle>Mol Med</addtitle><description>Both intrinsic and acquired multidrug resistance play an important role in the insurgence of tuberculosis. Detailed knowledge of the molecular basis of drug recognition and transport by multidrug transport systems is required for the development of new antibiotics that are not extruded or of inhibitors that block the multidrug transporter and allow traditional antibiotics to be effective.
We have undertaken the inventory of the drug transporters subfamily, included in the major facilitator superfamily (MFS), encoded by the complete genome of Mycobacterium tuberculosis (MTB). These proteins were identified on the basis of their characteristic stretches of amino acids and transmembrane segments (TMS) number.
Genome analysis and searches of homology between the identified transporters and proteins characterized in other organisms revealed 16 open reading frames encoding putative drug efflux pumps belonging to MFS. In the case of two of them, we also have demonstrated that they function as drug efflux proteins.</description><subject>Amino Acid Sequence</subject><subject>Anti-Infective Agents</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Biochemistry, Molecular Biology</subject><subject>Bioinformatics</subject><subject>Biological Physics</subject><subject>Biological Transport, Active</subject><subject>Cellular Biology</subject><subject>Chemical Sciences</subject><subject>Cloning, Molecular</subject><subject>Computational Biology</subject><subject>Computer Science</subject><subject>Conserved Sequence</subject><subject>Cristallography</subject><subject>Drug Resistance, Bacterial</subject><subject>Drug Resistance, Bacterial - physiology</subject><subject>Drug Resistance, Multiple</subject><subject>Evolution, Molecular</subject><subject>Gene Expression Regulation</subject><subject>Genome, Bacterial</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Multidrug Resistance-Associated Proteins</subject><subject>Multidrug Resistance-Associated Proteins - metabolism</subject><subject>Multigene Family</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Norfloxacin</subject><subject>Norfloxacin - pharmacology</subject><subject>Phylogeny</subject><subject>Physics</subject><subject>Sequence Homology, Amino Acid</subject><subject>Structural Biology</subject><issn>1076-1551</issn><issn>1528-3658</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1LJDEQhoOs-H3xB0if9iD0mo9OOn0RXNFVGPGi55Ck0zORdKfNhzD_fiMz6rqnKqqeeouqF4BTBH8hCNsLNUDSQAwJ3QEHiGJeE0b5j5LDltWIUrQPDmN8gRAj2tA9sI8wxRByfgD008pUY3bJ9iEvqxTkFGcfkgmxUsb5aWmnUvbVKF98qAaprbNJppLHPJswyNG6dWWn6mGtvZK6TNo8VikrE3R2Ptp4DHYH6aI52cYj8Hx783R9Vy8e_9xfXy1q3bQ81Y3ssGaYcd4xpDRTCBKtdG96ghQiBOK-6xU2SnNNZEOJREwp2jad0rQdGDkClxvdOavR9NpM5Rwn5mBHGdbCSyu-dya7Ekv_Jsrruo62RaDeCKz-G7u7WohZxmRyEJBg2nLI3lDhf24XBv-aTUxitFEb5-RkfI6ixRwhhnkBzzegDj7GYIZPdQTFu4fi9-2HhwU--_eML3RrGvkL4e6aQQ</recordid><startdate>200211</startdate><enddate>200211</enddate><creator>De Rossi, Edda</creator><creator>Arrigo, Patrizio</creator><creator>Bellinzoni, Marco</creator><creator>Silva, Pedro A E</creator><creator>Martín, Carlos</creator><creator>Aínsa, José A</creator><creator>Guglierame, Paola</creator><creator>Riccardi, Giovanna</creator><general>Feinstein Institute for Medical Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8887-6917</orcidid><orcidid>https://orcid.org/0000-0002-2661-169X</orcidid></search><sort><creationdate>200211</creationdate><title>The multidrug transporters belonging to major facilitator superfamily in Mycobacterium tuberculosis</title><author>De Rossi, Edda ; 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Detailed knowledge of the molecular basis of drug recognition and transport by multidrug transport systems is required for the development of new antibiotics that are not extruded or of inhibitors that block the multidrug transporter and allow traditional antibiotics to be effective.
We have undertaken the inventory of the drug transporters subfamily, included in the major facilitator superfamily (MFS), encoded by the complete genome of Mycobacterium tuberculosis (MTB). These proteins were identified on the basis of their characteristic stretches of amino acids and transmembrane segments (TMS) number.
Genome analysis and searches of homology between the identified transporters and proteins characterized in other organisms revealed 16 open reading frames encoding putative drug efflux pumps belonging to MFS. In the case of two of them, we also have demonstrated that they function as drug efflux proteins.</abstract><cop>England</cop><pub>Feinstein Institute for Medical Research</pub><pmid>12520088</pmid><doi>10.1007/bf03402035</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8887-6917</orcidid><orcidid>https://orcid.org/0000-0002-2661-169X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerNature Journals; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Amino Acid Sequence Anti-Infective Agents Anti-Infective Agents - pharmacology Biochemistry, Molecular Biology Bioinformatics Biological Physics Biological Transport, Active Cellular Biology Chemical Sciences Cloning, Molecular Computational Biology Computer Science Conserved Sequence Cristallography Drug Resistance, Bacterial Drug Resistance, Bacterial - physiology Drug Resistance, Multiple Evolution, Molecular Gene Expression Regulation Genome, Bacterial Life Sciences Molecular Sequence Data Multidrug Resistance-Associated Proteins Multidrug Resistance-Associated Proteins - metabolism Multigene Family Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Norfloxacin Norfloxacin - pharmacology Phylogeny Physics Sequence Homology, Amino Acid Structural Biology |
| title | The multidrug transporters belonging to major facilitator superfamily in Mycobacterium tuberculosis |
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