Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage
Human mesenchymal stem cells (hMSCs) have been shown to trans-differentiate into neuronal-like cells by culture in neuronal induction media, although the mechanism is not well understood. Topography can also influence cellular responses including enhanced differentiation of progenitor cells. As extr...
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| Veröffentlicht in: | Experimental cell research 2007-05, Vol.313 (9), p.1820-1829 |
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| creator | Yim, Evelyn K.F. Pang, Stella W. Leong, Kam W. |
| description | Human mesenchymal stem cells (hMSCs) have been shown to trans-differentiate into neuronal-like cells by culture in neuronal induction media, although the mechanism is not well understood. Topography can also influence cellular responses including enhanced differentiation of progenitor cells. As extracellular matrix (ECM)
in vivo comprises topography in the nanoscale, we hypothesize that nanotopography could influence stem cell differentiation into specific non-default pathways, such as transdifferentiation of hMSCs. Differentiation and proliferation of hMSCs were studied on nanogratings of 350 nm width. Cytoskeleton and nuclei of hMSCs were aligned and elongated along the nanogratings. Gene profiling and immunostaining showed significant up-regulation of neuronal markers such as microtubule-associated protein 2 (MAP2) compared to unpatterned and micropatterned controls. The combination of nanotopography and biochemical cues such as retinoic acid further enhanced the up-regulation of neuronal marker expressions, but nanotopography showed a stronger effect compared to retinoic acid alone on unpatterned surface. This study demonstrated the significance of nanotopography in directing differentiation of adult stem cells. |
| doi_str_mv | 10.1016/j.yexcr.2007.02.031 |
| format | Article |
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in vivo comprises topography in the nanoscale, we hypothesize that nanotopography could influence stem cell differentiation into specific non-default pathways, such as transdifferentiation of hMSCs. Differentiation and proliferation of hMSCs were studied on nanogratings of 350 nm width. Cytoskeleton and nuclei of hMSCs were aligned and elongated along the nanogratings. Gene profiling and immunostaining showed significant up-regulation of neuronal markers such as microtubule-associated protein 2 (MAP2) compared to unpatterned and micropatterned controls. The combination of nanotopography and biochemical cues such as retinoic acid further enhanced the up-regulation of neuronal marker expressions, but nanotopography showed a stronger effect compared to retinoic acid alone on unpatterned surface. This study demonstrated the significance of nanotopography in directing differentiation of adult stem cells.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2007.02.031</identifier><identifier>PMID: 17428465</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Biomarkers - metabolism ; Biomedical research ; Cartography ; Cell culture ; Cell Culture Techniques - methods ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Line ; Cell Lineage - drug effects ; Cell Lineage - physiology ; Cell Nucleus - physiology ; Cell Nucleus - ultrastructure ; Cell Polarity - physiology ; Cellular biology ; Cytoskeleton - physiology ; Cytoskeleton - ultrastructure ; Cytoskeleton rearrangement ; Extracellular Matrix - physiology ; Extracellular Matrix - ultrastructure ; Gene Expression Profiling ; GENE REGULATION ; Genetics ; Human mesenchymal stem cells ; Humans ; IN VIVO ; Mesenchymal Stem Cell Transplantation - methods ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - metabolism ; MICROTUBULES ; Nanoimprinting ; NANOSTRUCTURES ; Nanostructures - standards ; Nanotechnology ; Nanotechnology - methods ; Nanotopography ; Neuronal differentiation ; Neurons - cytology ; Neurons - drug effects ; Neurons - physiology ; PROTEINS ; RETINOIC ACID ; STEM CELLS ; TOPOGRAPHY ; Tretinoin - pharmacology</subject><ispartof>Experimental cell research, 2007-05, Vol.313 (9), p.1820-1829</ispartof><rights>2007 Elsevier Inc.</rights><rights>Copyright © 2007 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-4e688bbeac6340b3a9c2a43b9e38d6be3fe52717c21f9b1b36537f7613c151fc3</citedby><cites>FETCH-LOGICAL-c512t-4e688bbeac6340b3a9c2a43b9e38d6be3fe52717c21f9b1b36537f7613c151fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014482707000924$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17428465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20972156$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Yim, Evelyn K.F.</creatorcontrib><creatorcontrib>Pang, Stella W.</creatorcontrib><creatorcontrib>Leong, Kam W.</creatorcontrib><title>Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Human mesenchymal stem cells (hMSCs) have been shown to trans-differentiate into neuronal-like cells by culture in neuronal induction media, although the mechanism is not well understood. Topography can also influence cellular responses including enhanced differentiation of progenitor cells. As extracellular matrix (ECM)
in vivo comprises topography in the nanoscale, we hypothesize that nanotopography could influence stem cell differentiation into specific non-default pathways, such as transdifferentiation of hMSCs. Differentiation and proliferation of hMSCs were studied on nanogratings of 350 nm width. Cytoskeleton and nuclei of hMSCs were aligned and elongated along the nanogratings. Gene profiling and immunostaining showed significant up-regulation of neuronal markers such as microtubule-associated protein 2 (MAP2) compared to unpatterned and micropatterned controls. The combination of nanotopography and biochemical cues such as retinoic acid further enhanced the up-regulation of neuronal marker expressions, but nanotopography showed a stronger effect compared to retinoic acid alone on unpatterned surface. This study demonstrated the significance of nanotopography in directing differentiation of adult stem cells.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical research</subject><subject>Cartography</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cell Lineage - drug effects</subject><subject>Cell Lineage - physiology</subject><subject>Cell Nucleus - physiology</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cell Polarity - physiology</subject><subject>Cellular biology</subject><subject>Cytoskeleton - physiology</subject><subject>Cytoskeleton - ultrastructure</subject><subject>Cytoskeleton rearrangement</subject><subject>Extracellular Matrix - physiology</subject><subject>Extracellular Matrix - ultrastructure</subject><subject>Gene Expression Profiling</subject><subject>GENE REGULATION</subject><subject>Genetics</subject><subject>Human mesenchymal stem cells</subject><subject>Humans</subject><subject>IN VIVO</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>MICROTUBULES</subject><subject>Nanoimprinting</subject><subject>NANOSTRUCTURES</subject><subject>Nanostructures - standards</subject><subject>Nanotechnology</subject><subject>Nanotechnology - methods</subject><subject>Nanotopography</subject><subject>Neuronal differentiation</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>PROTEINS</subject><subject>RETINOIC ACID</subject><subject>STEM CELLS</subject><subject>TOPOGRAPHY</subject><subject>Tretinoin - pharmacology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO1DAQRS0EYpqBL0BCEUjsEvyK7SwYCY14SSOxANaW41Q6biX2YDsj-u9x6BavBata1LlVdesi9JTghmAiXh2aI3y3saEYywbTBjNyD-0I7nBNOaX30Q5jwmuuqLxAj1I6YIyVIuIhuiCSU8VFu0P7z0efJ8jOVt74kHJcbV4jpMr5YbXO76vBjSNE8NmZ7IKvwlhN62J8tUACb6fjYuYqZVgqC_O8CXOoPKwx-NKYnQezh8fowWjmBE_O9RJ9fff2y_WH-ubT-4_Xb25q2xKaaw5Cqb4HYwXjuGems9Rw1nfA1CB6YCO0VBJpKRm7nvRMtEyOUhBmSUtGyy7R1Wnu7dovMNhydjSzvo1uMfGog3H67453k96HO00xU52SZcCL04DyC6eTdRnsZIP3YHOBOklJKwr18rwmhm8rpKwXlzb7xkNYk5aYc17oAj7_BzyENZbHJE06LlSLBSsQO0E2hpQijL_uJVhvWeuD_pm13rLWmOqSdVE9-9Pqb8053AK8PgFQHn7nIG52SmIwuLi5GYL774IfIPO-qA</recordid><startdate>20070515</startdate><enddate>20070515</enddate><creator>Yim, Evelyn K.F.</creator><creator>Pang, Stella W.</creator><creator>Leong, Kam W.</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>20070515</creationdate><title>Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage</title><author>Yim, Evelyn K.F. ; Pang, Stella W. ; Leong, Kam W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-4e688bbeac6340b3a9c2a43b9e38d6be3fe52717c21f9b1b36537f7613c151fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical research</topic><topic>Cartography</topic><topic>Cell culture</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cell Lineage - drug effects</topic><topic>Cell Lineage - physiology</topic><topic>Cell Nucleus - physiology</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cell Polarity - physiology</topic><topic>Cellular biology</topic><topic>Cytoskeleton - physiology</topic><topic>Cytoskeleton - ultrastructure</topic><topic>Cytoskeleton rearrangement</topic><topic>Extracellular Matrix - physiology</topic><topic>Extracellular Matrix - ultrastructure</topic><topic>Gene Expression Profiling</topic><topic>GENE REGULATION</topic><topic>Genetics</topic><topic>Human mesenchymal stem cells</topic><topic>Humans</topic><topic>IN VIVO</topic><topic>Mesenchymal Stem Cell Transplantation - methods</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>MICROTUBULES</topic><topic>Nanoimprinting</topic><topic>NANOSTRUCTURES</topic><topic>Nanostructures - standards</topic><topic>Nanotechnology</topic><topic>Nanotechnology - methods</topic><topic>Nanotopography</topic><topic>Neuronal differentiation</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>PROTEINS</topic><topic>RETINOIC ACID</topic><topic>STEM CELLS</topic><topic>TOPOGRAPHY</topic><topic>Tretinoin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yim, Evelyn K.F.</creatorcontrib><creatorcontrib>Pang, Stella W.</creatorcontrib><creatorcontrib>Leong, Kam W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yim, Evelyn K.F.</au><au>Pang, Stella W.</au><au>Leong, Kam W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2007-05-15</date><risdate>2007</risdate><volume>313</volume><issue>9</issue><spage>1820</spage><epage>1829</epage><pages>1820-1829</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Human mesenchymal stem cells (hMSCs) have been shown to trans-differentiate into neuronal-like cells by culture in neuronal induction media, although the mechanism is not well understood. Topography can also influence cellular responses including enhanced differentiation of progenitor cells. As extracellular matrix (ECM)
in vivo comprises topography in the nanoscale, we hypothesize that nanotopography could influence stem cell differentiation into specific non-default pathways, such as transdifferentiation of hMSCs. Differentiation and proliferation of hMSCs were studied on nanogratings of 350 nm width. Cytoskeleton and nuclei of hMSCs were aligned and elongated along the nanogratings. Gene profiling and immunostaining showed significant up-regulation of neuronal markers such as microtubule-associated protein 2 (MAP2) compared to unpatterned and micropatterned controls. The combination of nanotopography and biochemical cues such as retinoic acid further enhanced the up-regulation of neuronal marker expressions, but nanotopography showed a stronger effect compared to retinoic acid alone on unpatterned surface. This study demonstrated the significance of nanotopography in directing differentiation of adult stem cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17428465</pmid><doi>10.1016/j.yexcr.2007.02.031</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Experimental cell research, 2007-05, Vol.313 (9), p.1820-1829 |
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| subjects | 60 APPLIED LIFE SCIENCES Biomarkers - metabolism Biomedical research Cartography Cell culture Cell Culture Techniques - methods Cell Differentiation - drug effects Cell Differentiation - physiology Cell Line Cell Lineage - drug effects Cell Lineage - physiology Cell Nucleus - physiology Cell Nucleus - ultrastructure Cell Polarity - physiology Cellular biology Cytoskeleton - physiology Cytoskeleton - ultrastructure Cytoskeleton rearrangement Extracellular Matrix - physiology Extracellular Matrix - ultrastructure Gene Expression Profiling GENE REGULATION Genetics Human mesenchymal stem cells Humans IN VIVO Mesenchymal Stem Cell Transplantation - methods Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism MICROTUBULES Nanoimprinting NANOSTRUCTURES Nanostructures - standards Nanotechnology Nanotechnology - methods Nanotopography Neuronal differentiation Neurons - cytology Neurons - drug effects Neurons - physiology PROTEINS RETINOIC ACID STEM CELLS TOPOGRAPHY Tretinoin - pharmacology |
| title | Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage |
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